A5063069385- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Biosensing Techniques and Applications
- COVID-19 Clinical Research Studies
- Cardiovascular Disease and Adiposity
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- Immune cells in cancer
- Long-Term Effects of COVID-19
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Computational Drug Discovery Methods
- Ferroptosis and cancer prognosis
NYU Langone Health
2024
New York University
2021-2023
Hinge Health
2023
Icahn School of Medicine at Mount Sinai
2019-2020
Cardiovascular Research Center
2019
Abstract Patients with coronavirus disease 2019 (COVID-19) present increased risk for ischemic cardiovascular complications up to 1 year after infection. Although the systemic inflammatory response severe acute respiratory syndrome 2 (SARS-CoV-2) infection likely contributes this risk, whether SARS-CoV-2 directly infects coronary vasculature and attendant atherosclerotic plaques remains unknown. Here we report that viral RNA is detectable replicates in lesions taken at autopsy from COVID-19...
Abstract The development of new immunotherapies to treat the inflammatory mechanisms that sustain atherosclerotic cardiovascular disease (ASCVD) is urgently needed. Herein, we present a path drug repurposing identify for ASCVD. integration time-of-flight mass cytometry and RNA sequencing identified unique signatures in peripheral blood mononuclear cells stimulated with ASCVD plasma. By comparing these large-scale gene expression data from LINCS L1000 dataset, drugs could reverse this...
COVID-19 patients present higher risk for myocardial infarction (MI), acute coronary syndrome, and stroke up to 1 year after SARS-CoV-2 infection. While the systemic inflammatory response infection likely contributes this increased cardiovascular risk, whether directly infects vasculature attendant atherosclerotic plaques locally promote inflammation remains unknown. Here, we report that viral RNA (vRNA) is detectable replicates in lesions taken at autopsy from with severe COVID-19....
Type 2 Diabetes (T2D) significantly increases the risk of cardiovascular diseases (CVDs), marked by severe plaque pathology resistant to standard cholesterol-lowering treatments. While roles innate immune system and macrophages in CVDs are well-established, T cell alterations, particularly involving cytotoxic CD4 cells (CD4 CTLs), remain unexplored human atherosclerotic plaques. In our study, we aimed identify phenotype function CTLs blood & tissue, context T2D. Using unbiased...
SUMMARY Atherosclerosis is driven by multifaceted contributions of the immune system within circulation and at vascular focal sites. Yet specific dysregulations atherosclerotic lesions that lead to clinical cerebro- cardiovascular complications (i.e. ischemic stroke myocardial infarction) are poorly understood. Here, using single-cell mass cytometry with Cellular Indexing Transcriptomes Epitopes Sequencing (CITE-seq) we found plaques were enriched in activated, differentiated, exhausted...
Introduction: Inflammation is a key driver of atherosclerosis. The identification new treatments and strategies targeting the specific inflammatory signaling that persists in optimally treated atherosclerotic patients remain an unmet clinical need. Hypothesis: integration time-of-flight mass cytometry (CyTOF), gene expression analysis, drug repositioning computational approaches applied to pre-clinical validation models disease using non-invasive imaging will help identify drugs tailored...
Abstract Patients with Type 2 diabetes (T2D) are at greater risk of atherosclerotic cardiovascular disease (ASCVD) and fail to fully respond standard lipid lowering drugs. While the role macrophages in T2D plaque pathology has been investigated, no studies have systematically investigated heterogeneity CD4 T cells human plaques how they cholesterol reduction. To identify specific cell alterations from patients T2D, we performed scRNAseq carotid 22 subjects (54.5% T2D) were being treated...
Introduction: Atherosclerotic plaques from patients with Type 2 Diabetes (T2D) present a more severe pathology than non-T2D (ND) and response to cholesterol-lowering is compromised. An increased level of circulating senescent CD8 T cells in T2D associated risk for myocardial infarction. However, whether infiltrate human atherosclerotic how they are regulated by lipid lowering unknown. Hypothesis: Senescent CD8+ circulation T2D, normalized aggressive T2D. Methods: We performed unbiased...
Atherogenesis is driven by the infiltration of immune cells into arterial wall where inflammation occurs and promotes plaque growth. While some plaques are stable, others vulnerable may rupture lead to stroke or myocardial infarction. The contribution specific cell types stability obscure. In our present study, we aim characterize repertoire human atherosclerosis using innovative single methods. We used mass cytometry (CyTOF) study tissue paired blood from symptomatic (TIA stoke < 6...
Introduction: The identification of new treatments for atherosclerosis (ACVD), a common complex disease, is daunting task because sets genes, rather than individual control cell functions. Using systems genetics, our team identified gene regulatory network (GRN42) that active in the human atherosclerotic arterial wall and involved regulation foam formation. Hypothesis: A network-driven drug repurposing approach combined with rigorous preclinical validation will identify uses existing drugs...
Introduction: Atherosclerotic cardiovascular disease (ACVD) is the main cause of morbidity and mortality in patients with type 2 diabetes (T2D). ACVD more severe diffuse T2D than subjects no (ND). Moreover, cholesterol reduction leads to less plaques resolution. While role macrophages plaque pathology has been investigated, studies have systematically investigated heterogeneity CD4 T cells human plaques. Hypothesis: Plaques a greater cell pathogenic phenotypes compared ND subjects. Methods:...
Introduction: Senescence is a key driver of age-related chronic inflammatory diseases including type 2 diabetes (T2D). Senescent T cells lose their ability to control tissue homeostasis and present aberrant cytokine cytotoxic responses that ultimately cause damage. Atherosclerotic plaques from T2D more severe pathology than patients with no (ND) circulating senescent CD8 are increased in associated myocardial infarction. However, whether accumulate atherosclerotic unknown. Hypothesis:...