A5051266485- Cancer-related Molecular Pathways
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and Catalytic Reactions
- Virus-based gene therapy research
- Cancer Research and Treatments
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Polyomavirus and related diseases
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Garlic and Onion Studies
- Genomics, phytochemicals, and oxidative stress
- Sulfur-Based Synthesis Techniques
- Epigenetics and DNA Methylation
- Enzyme function and inhibition
- Cellular transport and secretion
- Cell death mechanisms and regulation
- Heat shock proteins research
- Organometallic Compounds Synthesis and Characterization
Saarland University
2016-2025
Universitätsklinikum des Saarlandes
2007-2012
Damascus University
1998
Freie Universität Berlin
1993
Universität Ulm
1982-1992
University of Bonn
1974-1978
ABSTRACT By in situ hybridisation we have examined the expression of p53 during mouse embryogenesis from day 8.5 to 18.5 post coitum (p.c.). High levels mRNA were detected all cells p.c. and 10.5 embryo. However, at later stages development, became more pronounced differentiation specific tissues e.g. brain, liver, lung, thymus, intestine, salivary gland kidney. In undergoing terminal differentiation, level declined strongly. hybridisa-tion signals also observed postmitotic but not yet...
Cellular phosphoprotein p53, which seems to be a multifunctional protein, may assigned different structural subclasses. Recently established immortalized or transformed cell lines that overexpress p53 allowed us perform detailed analysis of the quaternary structure p53. By means sucrose density gradient centrifugation, we found in simian virus 40-transformed cells addition high-molecular-weight T-p53 complexes, low-molecular-weight forms. The level complexes within seemed determined by...
Protamine content is necessary for proper sperm chromatin condensation and subsequent male fertility. The exact effect of smoking on fertility remains controversial. objective this study was to evaluate the protamine in smoker non-smoker patients.Protamines 1 (P1) 2 (P2) were quantified by gel electrophoresis 53 smokers 63 non-smokers. Sperm DNA fragmentation analyzed employing terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) assay non-condensed evaluated using...
Among the human kinomes, protein kinase CK2 (formerly termed casein II) is considered to be essential, as it implicated in regulation of various cellular processes. Experiments with pharmacological inhibitors activity provide evidence that essential for development and differentiation. Therefore, present review addresses role during embryogenesis, neuronal, adipogenic, osteogenic myogenic differentiation established model cell lines, embryonic, neural mesenchymal stem cells. appears early...
Smoking is strongly associated with abnormalities in histone-to-protamine transition and alteration of protamine expression human spermatozoa. A proper to histone ratio is, however, essential for sperm chromatin maturity DNA integrity. Alterations these nuclear proteins were observed infertile men. The present prospective study aimed at evaluating the possible relationship among smoking, semen quality mature Histone H2B 1 (P1) 2 (P2) quantified using acid-urea polyacrylamide gel...
Protein kinase CK2 is composed of two regulatory β‐subunits and catalytic α‐ or α′‐subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the α‐, α′‐ β‐subunit. Immunofluorescence studies with revealed presence all three in cytoplasm weakly nucleus strong signals around nuclear membrane. Double staining experiments a co‐localisation tubulin. A direct association between α′‐subunit tubulin was confirmed by co‐immunoprecipitation as well...
The synthesis of a new class antisense oligonucleotide compounds with 3′-3′ and 5′-5′ end inversion (INV-oligonucleotides) is described. Besides the advantage simplicity synthesis, physico-chemical studies show that these do not disturb Watson—Crick base-pairing. INV-oligonucleotides have half-life 30 h in human serum. We they are capable inhibiting SV40 large T-antigen expression COS-1 cells, both vitro vivo, by modulation cellular oncoprotein p53 vitro.