A5017839495- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Lignin and Wood Chemistry
- Electrochemical sensors and biosensors
- Fungal Biology and Applications
- Biochemical and biochemical processes
- Biotin and Related Studies
- Fungal and yeast genetics research
Centro de Investigaciones Biológicas Margarita Salas
2017-2023
Consejo Superior de Investigaciones Científicas
2023
Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is preferred host for engineering fungal laccases. To assist difficult secretion active enzymes yeast, native signal peptide usually replaced preproleader S. alfa mating factor (MFα1). However, in most cases, only basal enzyme levels obtained. During directed evolution laccases fused α-factor...
The robustness of a high-redox potential laccase has been enhanced by swapping its second cupredoxin domain with that from another fungal laccase, which introduced pool neutral mutations in the protein sequence without affecting enzyme functionality. new showed outstanding stability to temperature, pH (2-9) and organic solvents, while maintaining ability oxidize substrates. By engineering signal peptide, secretion levels Saccharomyces cerevisiae were increased, allowed purify engineered for...
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation lignosulfonates with fungal oxidoreductases, such laccases, can increase by cross-linking lignin phenols. To advance this direction, we describe here development high-throughput screening...
During the kraft process to obtain cellulosic pulp from wood, most of lignin is removed by high-temperature alkaline cooking, released in black liquors and usually incinerated for energy. However, lignins are a valuable source phenolic compounds that can be valorized new bio-based products. The aim this work develop laccases capable working under extreme conditions high temperature pH, typical industrial conversion wood into fibreboard, order provide extremophilic biocatalysts depolymerising...
Lignins released in the black liquors of kraft pulp mills are an underutilised source aromatics. Due to their phenol oxidase activity, laccases from ligninolytic fungi suitable biocatalysts depolymerise lignins, which characterised by elevated phenolic content. However, alkaline conditions necessary solubilise lignins make it difficult use fungal whose activity is inherently acidic. We recently developed through enzyme-directed evolution high-redox potential active and stable at pH 10. Here,...