A5085767916- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Electrochemical sensors and biosensors
- Fungal and yeast genetics research
- Biochemical and biochemical processes
- Electrochemical Analysis and Applications
- Biotin and Related Studies
- Lignin and Wood Chemistry
- Avian ecology and behavior
- Malaria Research and Control
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Mosquito-borne diseases and control
- Bird parasitology and diseases
- Dye analysis and toxicity
- Trypanosoma species research and implications
- melanin and skin pigmentation
- Microbial Metabolic Engineering and Bioproduction
- Radiopharmaceutical Chemistry and Applications
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Animal Behavior and Reproduction
- Bacterial biofilms and quorum sensing
- Biofuel production and bioconversion
Centro de Investigaciones Biológicas Margarita Salas
2015-2024
Consejo Superior de Investigaciones Científicas
2015-2024
Universidad Complutense de Madrid
2015
San Francisco General Hospital
1995
Erythrocytic malaria parasites degrade hemoglobin as a principal source of amino acids for parasite protein synthesis. We have previously shown that Plasmodium falciparum trophozoite cysteine proteinase, now termed falcipain, is required degradation, and we hypothesized this proteinase responsible initial cleavages hemoglobin. To further evaluate the biological role expressed enzyme in bacterial viral expression systems. After baculovirus system, falcipain was enzymatically active had...
Oxidation of arylamines, such as aniline, is high industrial interest, and laccases have been proposed biocatalysts to replace harsh chemical oxidants. However, the reaction hampered by redox potential substrate at acid pH, enzyme engineering required improve oxidation. In this work, instead trying enzyme, we aim toward (transient) substrate's propose a more reliable strategy. We successfully combined computational approach with experimental validation rationally design an improved...
From laccase design to application of the overexpressed biocatalyst in an industrial environment for eco-friendly synthesis polyaniline and dyes.
Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is preferred host for engineering fungal laccases. To assist difficult secretion active enzymes yeast, native signal peptide usually replaced preproleader S. alfa mating factor (MFα1). However, in most cases, only basal enzyme levels obtained. During directed evolution laccases fused α-factor...
Abstract Saccharomyces cerevisiae plays an important role in the heterologous expression of array proteins due to its easy manipulation, low requirements and ability for protein post-translational modifications. The implementation preproleader secretion signal α-factor mating pheromone from this yeast contributes increase production yields by targeting foreign extracellular environment. use peptide combined with enzyme-directed evolution allowed us achieve otherwise difficult functional...
Polyaniline is a conductive polymer with distinctive optical and electrical properties. Its enzymatic synthesis an environmentally friendly alternative to the use of harsh oxidants extremely acidic conditions. 7D5L, high-redox potential laccase developed in our lab, biocatalyst choice for green polyaniline (emeraldine salt) due its superior ability oxidize aniline kinetic stability at required polymerization conditions (pH 3 presence anionic surfactants) as compared other fungal laccases....
Female birds may adjust their offspring phenotype to the specific requirements of environment by differential allocation physiologically active substances into yolks, such as androgens. Yolk androgens have been shown accelerate embryonic development, growth rate and competitive ability nestlings, but they can also entail immunological costs. The balance between costs benefits androgen is expected depend on nestling environment. We tested this hypothesis in a multibrooded passerine, spotless...
The robustness of a high-redox potential laccase has been enhanced by swapping its second cupredoxin domain with that from another fungal laccase, which introduced pool neutral mutations in the protein sequence without affecting enzyme functionality. new showed outstanding stability to temperature, pH (2-9) and organic solvents, while maintaining ability oxidize substrates. By engineering signal peptide, secretion levels Saccharomyces cerevisiae were increased, allowed purify engineered for...
ABSTRACT Ophiostoma piceae CECT 20416 is a dimorphic wood-staining fungus able to produce an extracellular sterol-esterase/lipase (OPE) that of great biotechnological interest. In this work, we have studied the morphological change from yeast hyphae, which associated with cell density-related mechanism known as quorum sensing (QS), and how affects secretion OPE. The data presented here confirm molecule E , -farnesol accumulates number growing within population. exogenous addition or spent...
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation lignosulfonates with fungal oxidoreductases, such laccases, can increase by cross-linking lignin phenols. To advance this direction, we describe here development high-throughput screening...
During the kraft process to obtain cellulosic pulp from wood, most of lignin is removed by high-temperature alkaline cooking, released in black liquors and usually incinerated for energy. However, lignins are a valuable source phenolic compounds that can be valorized new bio-based products. The aim this work develop laccases capable working under extreme conditions high temperature pH, typical industrial conversion wood into fibreboard, order provide extremophilic biocatalysts depolymerising...
Lignins released in the black liquors of kraft pulp mills are an underutilised source aromatics. Due to their phenol oxidase activity, laccases from ligninolytic fungi suitable biocatalysts depolymerise lignins, which characterised by elevated phenolic content. However, alkaline conditions necessary solubilise lignins make it difficult use fungal whose activity is inherently acidic. We recently developed through enzyme-directed evolution high-redox potential active and stable at pH 10. Here,...
Fungal laccases have great potential as biocatalysts oxidizing a variety of aromatic compounds using oxygen co-substrate. Here, the crystal structure 7D5 laccase (PDB 6H5Y), developed in Saccharomyces cerevisiae and overproduced Aspergillus oryzae, is compared with that wild type produced by basidiomycete PM1 (Coriolopsis sp.), PDB 5ANH. SAXS showed both enzymes form monomers solution, more oblate geometric due to heavier heterogeneous glycosylation. The enzyme presents superior catalytic...
ABSTRACT Lignocellulosic biomass, the most abundant natural resource on earth, can be used for cellulosic ethanol production but requires a pretreatment to improve enzyme access polymeric sugars while obtaining value from other components. γ‐Valerolactone (GVL) is promising candidate biomass since it renewable and bio‐based. In present work, effect of based GVL enzymatic saccharification white birch was evaluated at laboratory scale importance washing procedure subsequent demonstrated. Both...
Plant cell walls constitute a major defence barrier against pathogens, although it is unclear how specific wall components impact pathogen colonisation. Pathogens secrete degrading enzymes (CWDEs) to facilitate plant colonisation, but damaged, infected cells are often source of wall-derived oligosaccharides that trigger host immunity. The mechanisms by which pathogens minimize the release while colonizing remain be elucidated. We combined biochemical, molecular genetics and transcriptomic...