A5018016326- Wheat and Barley Genetics and Pathology
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Gut microbiota and health
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2022-2024
Universidad Politécnica de Madrid
2022-2024
Centre for Plant Biotechnology and Genomics
2022-2024
Consejo Superior de Investigaciones Científicas
2023
ETH Zurich
2015-2022
Summary Cultivar‐strain specificity in the wheat– Zymoseptoria tritici pathosystem determines infection outcome and is controlled by resistance genes on host side, many of which have been identified. On pathogen however, molecular determinants remain largely unknown. We used genetic mapping, targeted gene disruption allele swapping to characterise recognition new avirulence factor Avr3D1. then combined population comparative genomic analyses evolutionary trajectory Avr3D1 . specifically...
Fungal plant pathogens pose major threats to crop yield and sustainable food production if they are highly adapted their host the local environment. Variation in gene expression contributes phenotypic diversity within fungal species affects adaptation. However, very few cases of adaptive regulatory changes have been reported fungi underlying mechanisms remain largely unexplored. pathogen genomes plastic harbor numerous insertions transposable elements, which can potentially contribute...
, the causal agent of Septoria tritici blotch, is one Europe's most damaging wheat pathogens, causing significant economic losses. Genetic resistance a common strategy to control disease,
Accurate, high-throughput phenotyping for quantitative traits is a limiting factor progress in plant breeding. We developed an automated image analysis to measure resistance septoria tritici blotch (STB), globally important wheat disease, enabling identification of small chromosome intervals containing plausible candidate genes STB resistance. 335 winter cultivars were included replicated field experiment that experienced natural epidemic development by highly diverse but fungicide-resistant...
Dynamic changes in transcription profiles are key for the success of pathogens colonizing their hosts. In many pathogens, genes associated with virulence, such as effector genes, located regions genome that rich transposable elements and heterochromatin. The contribution chromatin modifications to gene expression remains largely unknown. Using a combination reporter gene-based approach immunoprecipitation, we show heterochromatic environment fungal plant pathogen Zymoseptoria tritici is...
Successful host colonization by plant pathogens requires the circumvention of defense responses, frequently through sequence modifications in secreted pathogen proteins known as avirulence factors (Avrs). Although Avr sequences are often polymorphic, contribution these polymorphisms to virulence diversity natural populations remains largely unexplored. We used molecular genetic tools determine how factor Avr3D1 wheat Zymoseptoria tritici contributed adaptive changes virulence. showed that...
ABSTRACT Zymoseptoria tritici , the causal agent of septoria blotch, is one Europe’s most damaging wheat pathogens, causing significant economic losses. Genetic resistance a common strategy to control disease, Stb6 being gene used for over 100 years in Europe. This study investigates molecular mechanisms underlying Stb6-mediated resistance. Utilizing confocal microscopy imaging, we identified that Z. epiphytic hyphae mainly accumulates corresponding avirulence factor AvrStb6 close proximity...
ABSTRACT Effector proteins are secreted by plant pathogens to enable host colonization. Typically, effector genes tightly regulated, have very low expression levels in axenic conditions, and strongly induced during Chromatin remodeling contributes the activation of planta still poorly known mechanisms. In this work, we investigated role histone acetylation gene derepression pathogens. We used Zymoseptoria tritici , a major pathogen wheat, as model determine lysine acetyltransferases (KATs)...
Summary Cultivar-strain specificity in the wheat- Zymoseptoria tritici pathosystem determines infection outcome and is controlled by resistance genes on host side, of which many have been identified. However, pathogen molecular determinants are largely unknown. We used genetic mapping, targeted gene disruption allele swapping to characterize recognition new avirulence factor Avr3D1. then combined population comparative genomic analyses estimate evolutionary trajectory Avr3D1 . specifically...
Zymoseptoria tritici is a highly damaging pathogen that causes high wheat yield losses in temperate climates. Z. emerged during the domestication of Fertile Crescent and has been extensively used as model system for population genetic genomic studies. New tools resources have provided better understanding molecular components involved wheat-Z. interaction, highlighted by cloning three resistance genes four avirulence genes. Despite considerable progress made last few years, mechanisms...
Plant cell walls constitute a major defence barrier against pathogens, although it is unclear how specific wall components impact pathogen colonisation. Pathogens secrete degrading enzymes (CWDEs) to facilitate plant colonisation, but damaged, infected cells are often source of wall-derived oligosaccharides that trigger host immunity. The mechanisms by which pathogens minimize the release while colonizing remain be elucidated. We combined biochemical, molecular genetics and transcriptomic...
Accurate, high-throughput phenotyping for quantitative traits is the limiting factor progress in plant breeding. We developed automated image analysis to measure resistance septoria tritici blotch (STB), a globally important wheat disease, enabling identification of small chromosome intervals containing plausible candidate genes STB resistance. 335 winter cultivars were included replicated field experiment that experienced natural epidemic development by highly diverse but...
Abstract Background Variation in gene expression contributes to phenotypic diversity within species and adaptation. However, very few cases of adaptive regulatory changes have been reported the mechanisms underlying variation remain largely unexplored. Fungal pathogen genomes are highly plastic harbour numerous insertions transposable elements, which can potentially contribute regulation. In this work we elucidated how elements melanin accumulation, a quantitative trait fungal pathogens that...
ABSTRACT Effector proteins are secreted by plant pathogens to enable host colonization. Typically, effector genes tightly regulated, have very low expression levels in axenic conditions, and strongly induced during Chromatin remodeling contributes the activation of planta still poorly known mechanisms. In this work we investigated role histone acetylation gene derepression pathogens. We used Zymoseptoria tritici , a major pathogen wheat, as model determine lysine acetyltransferases (KATs)...