A5016514055- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Biofuel production and bioconversion
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Plant pathogens and resistance mechanisms
- Polysaccharides Composition and Applications
- Mycorrhizal Fungi and Plant Interactions
- Phytochemicals and Antioxidant Activities
- Agriculture, Plant Science, Crop Management
- Geology and Paleoclimatology Research
- Seed Germination and Physiology
- Plant Surface Properties and Treatments
- Food composition and properties
- Powdery Mildew Fungal Diseases
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
Universidad de León
2013-2024
Universidad de León
2024
Universidad Andrés Bello
2019-2024
Every fungal cell is encapsulated in a wall, essential for viability, morphogenesis, and pathogenesis. Most knowledge of the wall composition fungi has focused on ascomycetes, especially human pathogens, but considerably less known about early divergent groups, such as species Zoopagomycota Mucoromycota phyla. To shed light evolutionary changes we studied monosaccharide 18 including diverging Basidiomycota Ascomycota phyla with focus those pathogenic lifestyles interactions plants. Our data...
Abstract Maize ( Zea mays L.) suspension‐cultured cells with up to 70% less cellulose were obtained by stepwise habituation dichlobenil (DCB), a biosynthesis inhibitor. Cellulose deficiency was accompanied marked changes in cell wall matrix polysaccharides and phenolics as revealed Fourier transform infrared (FTIR) spectroscopy. Cell compositional analysis indicated that the cellulose‐deficient walls showed an enhancement of highly branched cross‐linked arabinoxylans, well increased content...
Immune responses in plants are triggered by molecular patterns or elicitors, recognized plant pattern recognition receptors. Such the consequence of host-pathogen interactions, and response cascade activated after their perception is known as pattern-triggered immunity (PTI). Glucans have emerged key players PTI, but ability certain glucans to stimulate defensive remains understudied. This work focused on identifying novel glucan oligosaccharides patterns. The various microorganism-derived...
The use of plant-based preparations to replace chemical pesticides is a challenge for sustainable agriculture. Preparations from nettle (Urtica dioica L.) are good candidates, as the treatment common bean plants (Phaseolus vulgaris with aqueous suspensions reduced symptoms halo blight disease caused by Pseudomonas syringae pv. phaseolicola (Pph). aim present work was test if infusions (Ui) have similar effects and find out what activity behind this: antimicrobial, promotion natural defenses...
Upon imbibition, epidermal cells of Arabidopsis thaliana seeds release a mucilage formed mostly by pectic polysaccharides. The is composed mainly unbranched rhamnogalacturonan-I (RG-I), with low amounts cellulose, homogalacturonan, and traces xylan, xyloglucan, galactoglucomannan, galactan. pectin-rich composition the their simple extractability makes this structure good candidate to study biosynthesis polysaccharides modification. Here, we characterize phenotype mutant in...
Abstract Immune responses in plants are triggered by molecular patterns or elicitors, recognized plant pattern recognition receptors (PRRs). Such arise from host-pathogen interactions and the response cascade activated after their perception is known as pattern-triggered immunity (PTI). Glucans have emerged key players PTI, but certain glucans’ ability to stimulate defensive remains understudied. This work focused on identifying novel glucan oligosaccharides acting patterns. The of various...
Abstract Rhamnogalacturonan-I biosynthesis occurs in the lumen of Golgi apparatus, a compartment where UDP-Rhamnose and UDP-Galacturonic Acid are main substrates for synthesis backbone polymer pectin. Recent studies showed that UDP-Rha is transported from cytosol into apparatus by family six UDP-rhamnose/UDP-galactose transporters (URGT1-6). In this study, analysis adherent soluble mucilage (SM) Arabidopsis thaliana seeds revealed distinct roles URGT2, URGT4, URGT6 biosynthesis....
Abstract While evolutionary studies indicate that the most ancient groups of organisms on Earth likely descended from a common wall-less ancestor, contemporary lacking carbohydrate-rich cell surface are exceedingly rare. By developing wall to cover plasma membrane, cells were able withstand higher osmotic pressures, colonise new habitats and develop complex multicellular structures. This way, plants, algae microorganisms covered by wall, which can generally be defined as highly structure...
Cork sector plays an important economic role in several Mediterranean regions whose manufacturing process consists of stages involving waste generation, mainly industrial water or cork boiling wastewater (CBW). These products have traditionally been considered as simple pollutants, but CBW contains interesting compounds for recovery, however, their chemical characterization is long, difficult and expensive due to the high organic load. This work studied advanced valorization through a...
Abstract The mechanisms underlying susceptibility to and defense against Pseudomonas syringae (Pph) of the common bean (Phaseolus vulgaris) have not yet been clarified. To investigate these, 15-day-old plants variety Riñón were infected with Pph transcriptomic changes at 2 h 9 post-infection analysed. RNA-seq analysis showed an up-regulation genes involved in defense/signaling h, most them being down-regulated suggesting that inhibits reprogramming plant. This trend was also observed...
The cellulose biosynthesis inhibitor 2,6-dichlorobenzonitrile (DCB) has been widely used to gain insights into cell wall composition and architecture. Studies of changes during early habituation DCB can provide information on mechanisms that allow tolerance/habituation DCB. In this context, maize-cultured cells with a reduced amount (∼20%) were obtained by stepwise low concentrations. results reported here attempt elucidate the putative role an antioxidant strategy incipient habituation....
Plant cell walls constitute a major defence barrier against pathogens, although it is unclear how specific wall components impact pathogen colonisation. Pathogens secrete degrading enzymes (CWDEs) to facilitate plant colonisation, but damaged, infected cells are often source of wall-derived oligosaccharides that trigger host immunity. The mechanisms by which pathogens minimize the release while colonizing remain be elucidated. We combined biochemical, molecular genetics and transcriptomic...
Zhang Y, Yin Q, Qin W, Gao H, Du J, Chen Li Zhou G, Wu A-M. 2022. The Class II KNOX family members KNAT3 and KNAT7 redundantly participate in Arabidopsis seed coat mucilage biosynthesis. Journal of Experimental Botany 73, 3477–3495.
Nucleotide sugar transporters (NSTs) are Golgi-localized proteins that play a role in polysaccharide biosynthesis by transporting substrates (nucleotide sugars) from the cytosol into Golgi apparatus. In Arabidopsis, there is an NST subfamily of six members, called URGTs, which transport UDP-rhamnose and UDP-galactose vitro . URGTs very similar protein sequences, among them, URGT1 URGT2 highly conserved sequence also showed kinetic parameters toward Despite similarity function, mutants urgt1...