A5030575515- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
Universidad Andrés Bello
2015-2023
Millennium Science Initiative
2023
Millenium Institute Center for Genome Regulation
2023
Because they suck phloem sap and act as vectors for phytopathogenic viruses, aphids pose a threat to crop yields worldwide. Pectic homogalacturonan (HG) has been described defensive element plants during infections with phytopathogens. However, its role aphid infestation remains unexplored. Using immunofluorescence assays biochemical approaches, the HG methylesterification status associated modifying enzymes early stage of Arabidopsis (Arabidopsis thaliana) green peach (Myzus persicae) were...
UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and required for production seed mucilage. Following synthesis in cytosol, it transported into lumen Golgi apparatus, where converted to UDP-galacturonic (UDP-GalA), UDP-arabinose, UDP-xylose. To identify Golgi-localized UDP-GlcA transporter, we screened Arabidopsis thaliana mutants genes coding putative nucleotide sugar transporters altered mucilage, a structure rich GalA-containing polysaccharide...
The unfolded protein response (UPR) is a signaling pathway that activated when the workload of endoplasmic reticulum (ER) surpassed. IRE1 sensor involved in triggering UPR and plays key role unconventional splicing an mRNA leading to formation transcription factor up-regulates genes play restoring homeostasis ER. In plants, bZIP60 substrate for IRE1; however, questions such as what dynamics fate proteins encoded by spliced unspliced forms mRNA, remain unanswered. present work, we analyzed...
Upon imbibition, epidermal cells of Arabidopsis thaliana seeds release a mucilage formed mostly by pectic polysaccharides. The is composed mainly unbranched rhamnogalacturonan-I (RG-I), with low amounts cellulose, homogalacturonan, and traces xylan, xyloglucan, galactoglucomannan, galactan. pectin-rich composition the their simple extractability makes this structure good candidate to study biosynthesis polysaccharides modification. Here, we characterize phenotype mutant in...
Abstract Rhamnogalacturonan-I biosynthesis occurs in the lumen of Golgi apparatus, a compartment where UDP-Rhamnose and UDP-Galacturonic Acid are main substrates for synthesis backbone polymer pectin. Recent studies showed that UDP-Rha is transported from cytosol into apparatus by family six UDP-rhamnose/UDP-galactose transporters (URGT1-6). In this study, analysis adherent soluble mucilage (SM) Arabidopsis thaliana seeds revealed distinct roles URGT2, URGT4, URGT6 biosynthesis....
Abstract Polysaccharide methylation, especially that of pectin, is a common and important feature land plant cell walls. methylation takes place in the Golgi apparatus therefore relies on import S-adenosyl methionine (SAM) from cytosol into Golgi. However, to date, no SAM transporter has been identified plants. In this work, we studied major facilitator superfamily members Arabidopsis as putative transporters (GoSAMTs). Knock-out two most highly expressed GoSAMTs led strong reduction...
Introduction GoSAMTs play a role in the methylation of polysaccharides synthesized by Golgi. Pectin homogalacturonan (HG) methyl-esterification is essential for proper function this polysaccharide cell walls. In order to better understand HG biosynthesis, we analyzed mucilage gosamt mutants. Methods To determine GoSAMT1 and GoSAMT2 utilized epidermal cells seed coats, as these structures produce mucilage, which pectic matrix. We evaluated differences surface morphology quantified release....
Nucleotide sugar transporters (NSTs) are Golgi-localized proteins that play a role in polysaccharide biosynthesis by transporting substrates (nucleotide sugars) from the cytosol into Golgi apparatus. In Arabidopsis, there is an NST subfamily of six members, called URGTs, which transport UDP-rhamnose and UDP-galactose vitro . URGTs very similar protein sequences, among them, URGT1 URGT2 highly conserved sequence also showed kinetic parameters toward Despite similarity function, mutants urgt1...