- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Seed Germination and Physiology
- Postharvest Quality and Shelf Life Management
- Plant Genetic and Mutation Studies
- Plant and animal studies
Universidad de León
2014-2018
Universidad Nacional Autónoma de México
2015
Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture protocorms sections, obtained germination seeds. The top and bottom used as explants, were cultivated modified MS medium, added with different concentrations α-naph- thaleneacetic acid (NAA) 6-benzylaminopurine (BA). morphogenetic response both types formation protocorm like bodies (PLB’s), that gave place to new plantlets. Of two tested explants biggest PLB’s proto- corms sections. acclimatization...
From the germination of seeds Euchile mariae in modified MS medium, formation pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and his first leaf primordium, they were used like explants induce a morphogenetic response. Through vitro culture top bottom protocorms sections, obtained differentiated structures asexual origin. Their morphology similar seeds, this way we call them protocorm bodies (PLB’s). histological analysis it possible reveal that PLB’s turned...