Alfonso Gonzalo De la Rubia

ORCID: 0000-0002-5383-6034
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About
Contact & Profiles
Research Areas
  • Plant pathogens and resistance mechanisms
  • Plant-Microbe Interactions and Immunity
  • Bacterial Genetics and Biotechnology
  • Redox biology and oxidative stress
  • Polysaccharides and Plant Cell Walls
  • Plant Pathogenic Bacteria Studies
  • Legume Nitrogen Fixing Symbiosis
  • Plant nutrient uptake and metabolism
  • Plant Reproductive Biology
  • Enzyme Structure and Function
  • Adsorption and biosorption for pollutant removal
  • Polyamine Metabolism and Applications
  • Arsenic contamination and mitigation
  • Analytical chemistry methods development
  • Plant responses to water stress
  • Nematode management and characterization studies
  • Chromium effects and bioremediation
  • Cancer Research and Treatments
  • Polysaccharides Composition and Applications
  • Environmental remediation with nanomaterials
  • Antibiotic Resistance in Bacteria

Universidad de León
2016-2025

ETH Zurich
2022-2023

Hydrogen peroxide (H 2 O ) is a strong oxidant capable of oxidizing cysteinyl thiolates, yet only few cysteine-containing proteins have exceptional reactivity toward H . One such example the prokaryotic transcription factor OxyR, which controls antioxidant response in bacteria, and specifically rapidly reduces In this study, we present crystallographic evidence for -sensing mechanism -dependent structural transition Corynebacterium glutamicum OxyR by capturing reduced -bound structures...

10.1073/pnas.1807954115 article EN cc-by-nc-nd Proceedings of the National Academy of Sciences 2018-11-21

Abstract Pine needles are specialized leaves bearing distinctive anatomical features whose function is to minimize water loss. At the molecular level, needle specialization expected lead heterogeneity in cell wall (CW) composition. By immunohistochemical analysis of CW epitopes maritime pine ( Pinus pinaster ) needles, we described variability composition all tissues, discussing its possible relationship with function. Cells transport, tracheids, transfusion and endodermis, together...

10.1007/s00709-025-02041-5 article EN cc-by PROTOPLASMA 2025-02-18

The Mycobacterium tuberculosis rv2466c gene encodes an oxidoreductase enzyme annotated as DsbA. It has a CPWC active-site motif embedded within its thioredoxin fold domain and mediates the activation of prodrug TP053, thienopyrimidine derivative that kills both replicating nonreplicating bacilli. However, mode action actual enzymatic function in M. have remained enigmatic. In this study, we report Rv2466c is essential for bacterial survival under H2O2 stress. Further, discovered lacks...

10.1074/jbc.m117.797837 article EN cc-by Journal of Biological Chemistry 2017-06-16

The cell wall (CW) is a dynamic structure extensively remodeled during plant growth and under stress conditions, however little known about its roles the immune system priming, especially in crops. In order to shed light on such process, we used Phaseolus vulgaris-Pseudomonas syringae (Pph) pathosystem priming capacity of 2,6-dichloroisonicotinic acid (INA). first instance confirmed that INA-pretreated plants were more resistant Pph, which was line with enhanced production H2O2 primed after...

10.3390/plants10081514 article EN Plants 2021-07-23

Abstract The mechanisms underlying susceptibility to and defense against Pseudomonas syringae (Pph) of the common bean (Phaseolus vulgaris) have not yet been clarified. To investigate these, 15-day-old plants variety Riñón were infected with Pph transcriptomic changes at 2 h 9 post-infection analysed. RNA-seq analysis showed an up-regulation genes involved in defense/signaling h, most them being down-regulated suggesting that inhibits reprogramming plant. This trend was also observed...

10.1093/jxb/erad362 article EN Journal of Experimental Botany 2023-09-15

Common bean (Phaseolus vulgaris) is attacked by several pathogens such as the biotrophic gamma-proteobacterium Pseudomonas syringae pv. phaseolicola. To study P. phaseolicola-bean interaction during first stages of infection, leaf discs a susceptible cultivar Riñón were infected with pathogenic Using this experimental system, we tested six new putative wall-associated kinase (WAK) receptors, previously identified in silico. These vulgaris WAKs (PvWAKs) showed high protein sequence homology...

10.1094/phyto-10-20-0449-r article EN Phytopathology 2021-05-04

Halo blight disease of beans (Phaseolus vulgaris L.), caused by the bacterium Pseudomonas syringae pv. phaseolicola (Pph), is responsible for severe losses in crop production worldwide. As current agronomic techniques used are not effective, it necessary to search new ones which may prevent common bean. In this study, we challenged four plant-based preparations (PBPs), with no other uses, as they come from industrial waste (grapevine pomace (RG) and hop residue (RH)) or wild plants (Urtica...

10.3390/agronomy12010063 article EN cc-by Agronomy 2021-12-28

Abstract The susceptibility of common bean varieties to Pseudomonas syringae pv. phaseolicola (Pph) has been well-documented. However, the molecular mechanism that drives this not clarified yet. In an attempt understand process, 15-day-old plants, variety riñón, were infected with Pph analyze transcriptomic changes during first steps infection (at 2 and 9 h). RNA-seq analysis showed upregulation defense-and signaling-related genes at 2h, most them being downregulated 9h, suggesting would...

10.1101/2022.12.19.521100 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2022-12-21
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