A5049832487- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Inhalation and Respiratory Drug Delivery
- Antimicrobial Peptides and Activities
- Nanoparticle-Based Drug Delivery
- Cystic Fibrosis Research Advances
- Pancreatic function and diabetes
- Electrospun Nanofibers in Biomedical Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tissue Engineering and Regenerative Medicine
- Cervical Cancer and HPV Research
- Lipid Membrane Structure and Behavior
- Diabetes Management and Research
- Immune cells in cancer
- Neonatal Respiratory Health Research
- Zebrafish Biomedical Research Applications
- 3D Printing in Biomedical Research
- Ultrasound and Hyperthermia Applications
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Dysphagia Assessment and Management
- Advancements in Transdermal Drug Delivery
- Boron Compounds in Chemistry
Beijing Luhe Hospital Affiliated to Capital Medical University
2025
Harvard University
2016-2023
Stanford University
2021
Institute for Neurodegenerative Disorders
2020-2021
University of California, San Francisco
2020-2021
Johns Hopkins University
2007-2017
Johns Hopkins Medicine
2012-2017
Massachusetts Institute of Technology
2013-2016
Boston Children's Hospital
2013-2016
University of Delaware
2003
Protective mucus coatings typically trap and rapidly remove foreign particles from the eyes, gastrointestinal tract, airways, nasopharynx, female reproductive thereby strongly limiting opportunities for controlled drug delivery at mucosal surfaces. No synthetic system composed of biodegradable polymers has been shown to penetrate highly viscoelastic human mucus, such as non-ovulatory cervicovaginal a significant rate. We prepared nanoparticles diblock copolymer poly(sebacic acid)...
A glucose-responsive closed-loop insulin delivery system represents the ideal treatment of type 1 diabetes mellitus. In this study, we develop uniform injectable microgels for controlled release insulin. Monodisperse (256 ± 18 μm), consisting a pH-responsive chitosan matrix, enzyme nanocapsules, and recombinant human insulin, were fabricated through one-step electrospray procedure. Glucose-specific enzymes covalently encapsulated into nanocapsules to improve enzymatic stability by protecting...
Incomplete coverage and short duration of action limit the effectiveness vaginally administered drugs, including microbicides, for preventing sexually transmitted infections. We investigated vaginal distribution, retention, safety nanoparticles with surfaces modified to enhance transport through mucus. show that mucus-penetrating particles (MPPs) provide uniform distribution over epithelium, whereas conventional (CPs) are mucoadhesive aggregated by mouse mucus, leading poor distribution....
Mucoadhesive particles (MAP) have been widely explored for pulmonary drug delivery because of their perceived benefits in improving particle residence the lungs. However, retention adhesively trapped airway mucus may be limited by physiologic clearance mechanisms. In contrast, that avoid mucoadhesion and diameters smaller than mesh spacings rapidly penetrate layers [mucus-penetrating (MPP)], which we hypothesized would provide prolonged lung compared to MAP. We vivo behaviors variously...
Since its discovery and isolation, exogenous insulin has dramatically changed the outlook for patients with diabetes. However, even when strictly follow an regimen, serious complications can result as experience both hyperglycemic hypoglycemic states. Several chemically or genetically modified insulins have been developed that tune pharmacokinetics of activity personalized therapy. Here, we demonstrate a strategy chemical modification intended to promote long-lasting glucose-responsive...
Injectable hydrogels have been widely used for a number of biomedical applications. Here, we report new strategy to form an injectable and glucose-responsive hydrogel using the boronic acid–glucose complexation. The ratio acid glucose functional groups is critical formation. In our system, polymers with 10–60% acid, balance being glucose-modified, are favorable hydrogels. These shear-thinning self-healing, recovering from shear-induced flow gel state within seconds. More importantly, these...
In vivo implantation of sterile materials and devices results in a foreign body immune response leading to fibrosis implanted material. Neutrophils, one the first cells be recruited sites, have been suggested contribute establishment inflammatory microenvironment that initiates fibrotic response. However, precise numbers roles neutrophils remains unclear. Using mouse model peritoneal microcapsule implantation, we show 30–500 fold increased neutrophil presence exudates implants. We...
Safe and effective delivery is required for siRNA mRNA-based therapeutics to reach their potential. Here, we report on the development of poly(glycoamidoamine) brush nanoparticles as vehicles mRNA. These polymers were capable significant against FVII mRNA-encoding erythropoietin (EPO) in mice. Importantly, these well-tolerated at dose based analysis tissue histology, systemic cytokine levels, liver enzyme chemistry. The polymer reported here are promising therapeutic applications.
Local delivery of chemotherapeutics in the cervicovaginal tract using nanoparticles may reduce adverse side effects associated with systemic chemotherapy, while improving outcomes for early-stage cervical cancer. It is hypothesized here that drug-loaded rapidly penetrate mucus (CVM) lining female reproductive will more effectively deliver their payload to underlying diseased tissues a uniform and sustained manner compared do not efficiently CVM. Paclitaxel-loaded are developed, composed...
Here, efforts toward the development of a microneedle-based glucose sensor or "smart patch" for intradermal sensing are described. Metallic microneedle array electrodes, conducting polymers, and oxidase form platform. This work represents first steps painless, transdermal-sensing devices continuous monitoring As service to our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized online delivery, but not...
For effective airway gene therapy of cystic fibrosis (CF), inhaled carriers must first penetrate the hyperviscoelastic sputum covering epithelium. Whether clinically studied can CF remains unknown. Here, we measured diffusion a tested nonviral carrier, composed poly-l-lysine conjugated with 10 kDa polyethylene glycol segment (CK30PEG10k). We found that CK30PEG10k/DNA nanoparticles were trapped in sputum. To improve carrier across sputum, adjuvant regimens consisting N-acetylcysteine (NAC),...
New lipid-like nanomaterials are developed to simultaneously regulate expression of multiple genes. Self-assembled nanoparticles capable efficiently encapsulating pDNA and siRNA. These shown induce simultaneous gene silencing both in vitro vivo. As a service our authors readers, this journal provides supporting information supplied by the authors. Such materials peer reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical support issues arising from (other...
Abstract In eukaryotes, secretory proteins are folded and assembled in the endoplasmic reticulum (ER). Many heterologous retained ER due to suboptimal folding conditions. We previously reported that secretion of Pyrococcus furiosus β‐glucosidase Saccharomyces cerevisiae resulted accumulation a large fraction inactive ER. this work, we determine effect introducing additional genes ER‐resident yeast proteins, Kar2p (binding protein [BiP]) disulfide isomerase (PDI), on relieving bottleneck....
Angiogenesis is the formation of neovasculature from a pre-existing vascular network. Progression solid tumors including lung cancer angiogenesis-dependent. We previously introduced bioinformatics-based methodology to identify endogenous anti-angiogenic peptide sequences, and validated these predictions in vitro human umbilical vein endothelial cell (HUVEC) proliferation migration assays.One family peptides with high activity derived alpha-fibrils type IV collagen. Based on results...
Parkinson’s disease (PD) is a common neurodegenerative disorder without effective disease-modifying therapeutics. Here, we establish chemogenetic dopamine (DA) neuron ablation model in larval zebrafish with mitochondrial dysfunction and robustness suitable for high-content screening. We use this system to conduct an vivo DA imaging-based chemical screen identify the Renin-Angiotensin-Aldosterone System (RAAS) inhibitors as significantly neuroprotective. Knockdown of angiotensin receptor 1 (...
Abstract Objective Sepsis represents a leading cause of global mortality, defined by dysregulated inflammatory response. This study aims to investigate the potential causal associations between circulating proteins and sepsis risk using two-sample Mendelian randomization (MR) approach. Methods Publicly available summary statistics from genome-wide association studies (GWAS) were used in this study. Genetic instruments for protein derived GWAS meta-analysis 11 cohorts encompassing 14,824...
Abstract Using live‐cell confocal microscopy and particle tracking technology, the simultaneous transport of intracellular vesicles endo‐lysosomal pathway nonviral polyethylenimine (PEI)/DNA nanocomplexes was investigated. Due to potential problems associated with use acid‐sensitive probes in combination a gene vector that is hypothesized buffer pH vesicles, biological location PEI/DNA vectors revealed by probing their trafficking cells expressing fluorescent versions either early endosome...