A5020791705- Enzyme Production and Characterization
- Zebrafish Biomedical Research Applications
- Microbial Metabolites in Food Biotechnology
- Ginseng Biological Effects and Applications
- Advanced Fluorescence Microscopy Techniques
- Biofuel production and bioconversion
- Renin-Angiotensin System Studies
- Natural product bioactivities and synthesis
- Food composition and properties
- Neuroinflammation and Neurodegeneration Mechanisms
- Enzyme Catalysis and Immobilization
- Retinal Development and Disorders
- melanin and skin pigmentation
- Curcumin's Biomedical Applications
- Enzyme-mediated dye degradation
- Antioxidant Activity and Oxidative Stress
- Biochemical Analysis and Sensing Techniques
- Phytochemicals and Antioxidant Activities
- COVID-19 Clinical Research Studies
- Stress Responses and Cortisol
- Microbial Metabolism and Applications
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Pharmacological Effects of Natural Compounds
University of California, San Francisco
2014-2024
University of California, San Diego
2009-2011
Parkinson’s disease (PD) is a common neurodegenerative disorder without effective disease-modifying therapeutics. Here, we establish chemogenetic dopamine (DA) neuron ablation model in larval zebrafish with mitochondrial dysfunction and robustness suitable for high-content screening. We use this system to conduct an vivo DA imaging-based chemical screen identify the Renin-Angiotensin-Aldosterone System (RAAS) inhibitors as significantly neuroprotective. Knockdown of angiotensin receptor 1 (...
Hydroquinone galactoside (HQ-Gal) as a potential skin whitening agent was synthesized by the reaction of lactase (β-galactosidase) from Kluyveromyces lactis, Aspergillus oryzae, Bacillus circulans, and Thermus sp. with lactose donor HQ an acceptor. Among these lactases, acceptor involving K. lactis showed higher conversion ratio to HQ-Gal (60.27%). purified using butanol partitioning silica gel column chromatography. The structure determined nuclear magnetic resonance, ionic product observed...
This study explores the formulation of self-micelle ginsenoside saponins from Panax ginseng with curcuminoids turmeric. Although ginsenosides are hydrophilic and poorly membrane-permeable, they increase solubility absorption insoluble compounds such as curcuminoids. The optimized ginsenoside-curcuminoid (GS-CUR) achieved a water 2,450 µg/mL using Response Surface Methodology (RSM) at 42.9 % (v/v) in ethanol, 389.7 mg/mL ginsenosides, 9.06 Analytical techniques SEM, X-ray diffraction, FTIR...
Abstract Among cases of SARS-CoV-2 infections that result in serious conditions or death, many have pre-existing such as hypertension and are on renin–angiotensin–aldosterone system (RAAS) inhibitors. The angiotensin-converting-enzyme-2 (ACE2), a key protein the RAAS pathway, also mediates cellular entry SARS-CoV-2. inhibitors might affect expression levels ace2 , which could impact patient susceptibility to However, multi-organ-specific information is currently lacking no species other than...
Abstract Compound K (CK), a ginsenoside with high bioavailability, is present at low levels in wild-simulated ginseng leaves (WSGL). WSGL contains the CK precursors, Rd and F2, amounts up to 26.4 ± 0.4 24.1 1.9 mg/g extract, respectively. In this study, production WGSL reached 25.9 1.0 extract following treatment Viscozyme, Celluclast 1.5 L, Pectinex Ultra SP-L, their combination. The antioxidant activities indicated by oxygen radical absorbance capacity, ferric reducing power, ABTS- DPPH...
Parkinson’s disease (PD) is the second most common neurodegenerative disorder with prominent dopamine (DA) neuron degeneration. PD affects millions of people worldwide, but currently available therapies are limited to temporary relief symptoms. As an effort discover disease-modifying therapeutics, we have conducted a screen 1,403 bioactive small molecule compounds using in vivo whole organism screening assay transgenic larval zebrafish. The model expresses bacterial enzyme nitroreductase...
Background: Production of wheat bran (WB) for human consumption is estimated to be about 90 million tons per year. WB contains abundant source dietary fiber, minerals, vitamins, and bioactive compounds. a by-product milling carbohydrate (60%), protein (12%), fat (0.5%), minerals (2%), compounds such as phenolic acids, arabinoxylans, flavonoids, caroteinoids alkylresorcinol phytosterols. These are known health promoting properties controlling glycemic index, reducing plasma cholesterol level,...
Whole-organism-based screen holds promise for discovering biologically active compounds. However, high-content imaging is challenging due to the difficulty of positioning live animals and individual variability neuron counts. Here, we present a protocol identify neuroprotective compounds dopaminergic neurons in zebrafish using an image-based small-molecule screen. We describe steps raising larvae, agarose embedding, treatment induce neurodegeneration. then detail procedures confocal imaging,...