A5014598631- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Cellular transport and secretion
- Pharmacogenetics and Drug Metabolism
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- Biochemical Acid Research Studies
- Microbial Natural Products and Biosynthesis
- Lipid metabolism and biosynthesis
- Nuclear Structure and Function
- Asymmetric Hydrogenation and Catalysis
- Polyamine Metabolism and Applications
- Click Chemistry and Applications
- Steroid Chemistry and Biochemistry
- Ion Transport and Channel Regulation
- Polysaccharides and Plant Cell Walls
- Glycogen Storage Diseases and Myoclonus
- Diet and metabolism studies
- Biochemical and Molecular Research
University of Manchester
2021-2024
Medical Research Council
2004-2013
Imperial College London
2003-2013
Hammersmith Hospital
2004
Newcastle University
1991-1992
Abstract Selective, one‐step C−H activation of fatty acids from biomass is an attractive concept in sustainable chemistry. Biocatalysis has shown promise for generating high‐value hydroxy acids, but to date enzyme discovery relied on laborious screening and produced limited hits, which predominantly oxidise the subterminal positions acids. Herein we show that ancestral sequence reconstruction (ASR) effective tool explore sequence‐activity landscape a family multidomain, self‐sufficient P450...
The kinase-activator protein (KAP) of pyruvate dehydrogenase complex (PDC) has been purified approx. 2250-fold from high-speed supernatants mitochondrial extracts the liver 48 h-starved rats. Purified KAP demonstrates kinase activity towards both E1 component PDC and a synthetic peptide corresponding to major phosphorylation site on E1. Furthermore, activities co-fractionate through several stages purification have same apparent mass. We conclude that is not distinct protein, but which...
β-D-Galactofuranose (Galf) and its polysaccharides are found in bacteria, fungi protozoa but do not occur mammalian tissues, thus represent a specific target for anti-pathogenic drugs. Understanding the enzymatic degradation of these is therefore great interest, identity fungal enzymes with exclusively galactofuranosidase activity has so far remained elusive. Here we describe identification characterization from industrially important fungus Aspergillus niger. Analysis glycoside hydrolase...
Abstract Selective, one‐step C−H activation of fatty acids from biomass is an attractive concept in sustainable chemistry. Biocatalysis has shown promise for generating high‐value hydroxy acids, but to date enzyme discovery relied on laborious screening and produced limited hits, which predominantly oxidise the subterminal positions acids. Herein we show that ancestral sequence reconstruction (ASR) effective tool explore sequence‐activity landscape a family multidomain, self‐sufficient P450...
Biokatalytische Modifikationen bieten attraktive Methoden für die selektive Funktionalisierung von komplexen Molekülen. Inmitten einer ständig wachsenden Zahl Enzymen sind heute modulare Werkzeugkästen enzymatischer Transformationen in der Synthese verfügbar. Eine große Biokatalysatoren ist Lage, vielzählige Diversifikationen vorzunehmen, z. B. Wirkstoffentwicklung. In ihrem Aufsatz auf S. 16962 stellen Nicholas J. Turner, Christian Schnepel et al. derzeitige Bandbreite vor.
Selective, one-step C-H activation of fatty acids from biomass is an attractive concept in sustainable chemistry. Biocatalysis has shown promise for generating high-value hydroxy but to date enzyme discovery relied on laborious screening and produced limited hits, which predominantly oxidise the sub-terminal positions acids. Here we show that ancestral sequence reconstruction (ASR) effective tool explore sequence-activity landscape a family multi-domain, self-sufficient P450 monooxygenases....
Abstract β-D-Galactofuranose (Gal f ) and its polysaccharides are found in bacteria, fungi protozoa but do not occur mammalian tissues, thus represent a specific target for anti-pathogenic drugs. Understanding the enzymatic degradation of these is therefore great interest, identity fungal enzymes with exclusively galactofuranosidase activity has so far remained elusive. Here we describe identification characterization from industrially important fungus Aspergillus niger . Phylogenetic...