A5017888348- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Computational Drug Discovery Methods
- Enzyme Catalysis and Immobilization
- Analytical Chemistry and Chromatography
- Chemical Reactions and Isotopes
- Eicosanoids and Hypertension Pharmacology
- Fungal Biology and Applications
- Steroid Chemistry and Biochemistry
- Cancer Treatment and Pharmacology
- CRISPR and Genetic Engineering
- Estrogen and related hormone effects
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hormonal Regulation and Hypertension
- Carcinogens and Genotoxicity Assessment
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects and Toxicity Studies
- Genetics, Bioinformatics, and Biomedical Research
- RNA and protein synthesis mechanisms
- Asymmetric Hydrogenation and Catalysis
- Genomics and Phylogenetic Studies
- Various Chemistry Research Topics
- Microstructure and mechanical properties
- Biochemical and Molecular Research
The University of Queensland
2015-2024
AstraZeneca (Sweden)
2020-2024
Robert Bosch (Germany)
2024
Vanderbilt University
1993-2022
Park Centre for Mental Health
2006
University of Sheffield
2005
Princess Alexandra Hospital
2005
Royal Hallamshire Hospital
1990-2005
Osaka Prefectural Institute of Public Health
1999-2001
Saitama Cancer Center
2000
Indole is a product of tryptophan catabolism by gut bacteria and absorbed into the body in substantial amounts. The compound known to be oxidized indoxyl excreted urine as (3-hydroxyindole) sulfate. Further oxidation dimerization leads formation indigoid pigments. We report definitive identification pigments indigo indirubin products human cytochrome P450 (P450)-catalyzed metabolism indole visible, 1H NMR, mass spectrometry. 2A6 was most active these two pigments, followed P450s 2C19 2E1....
The cytochrome P450 (P450)-mediated biotransformation of tamoxifen is important in determining both the clearance drug and its conversion to active metabolite,<i>trans</i>-4-hydroxytamoxifen. Biotransformation by forms expressed extrahepatically, such as breast endometrium, may be particularly tissue-specific effects tamoxifen. Moreover, serve a useful probe examine regioselectivity different forms. Tamoxifen metabolism was investigated vitro using recombinant human P450s. Forms CYP1A1, 1A2,...
The glucose–methanol–choline (GMC) superfamily is a large and functionally diverse family of oxidoreductases that share common structural fold. Fungal members this are characterised relevant for lignocellulose degradation include aryl-alcohol oxidoreductase, alcohol oxidase, cellobiose dehydrogenase, glucose pyranose which together form AA3 the auxiliary activities in CAZy database carbohydrate-active enzymes. Overall, little known about extant sequence space these GMC their phylogenetic...
The measurement of the proportion elements atomic numbers higher than 20 in steels by X-ray fluorescence using a Geiger-counter spectrometer has been investigated, primarily with view to determining suitability method as rapid means analysis during steel-making process, particularly for high alloy steels. A monitoring system compensate fluctuations tube output is described and shown increase accuracy, though gives variances theoretical once obtained assuming Poissonian distribution counting...
Ancestral sequence reconstruction is a technique that gaining widespread use in molecular evolution studies and protein engineering. Accurate requires the ability to handle appropriately large numbers of sequences, as well insertion deletion (indel) events, but available approaches exhibit limitations. To address these limitations, we developed Graphical Representation Sequence Predictions (GRASP), which efficiently implements maximum likelihood methods enable inference ancestors families...
Abstract Selective, one‐step C−H activation of fatty acids from biomass is an attractive concept in sustainable chemistry. Biocatalysis has shown promise for generating high‐value hydroxy acids, but to date enzyme discovery relied on laborious screening and produced limited hits, which predominantly oxidise the subterminal positions acids. Herein we show that ancestral sequence reconstruction (ASR) effective tool explore sequence‐activity landscape a family multidomain, self‐sufficient P450...
Cytochrome P450 1B1 (CYP1B1) participates in the metabolic activation of a number procarcinogens including benzo[a]pyrene and hydroxylation 17β-estradiol at C-4 position. In this study, we investigated association between CYP1B1 genetic polymorphism breast or lung cancer incidence. The Ala-Ser codon 119 presumed substrate recognition site 1 was significantly associated with incidence squamous cell carcinoma lung. On other hand, Leu-Val 432 did not show any to cancers. An allele containing...
Recombinant human enzymes expressed in membranes obtained from Escherichia coli transformed with cytochrome P450 (P450) and NADPH−P450 reductase cDNAs were used to identify the that are most active catalyzing oxidative transformation of benzo[a]pyrene vitro. Activation genotoxic products cause induction umu gene expression Salmonella typhimurium NM2009 by 1A1 1B1 was found be enhanced inclusion purified epoxide hydrolase (isolated rat or livers) reaction mixture. High-performance liquid...