A5068402994- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Cancer Cells and Metastasis
- Renal and related cancers
- Sperm and Testicular Function
- Estrogen and related hormone effects
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Organ and Tissue Transplantation Research
- Urological Disorders and Treatments
- Pregnancy and Medication Impact
- Animal Genetics and Reproduction
- Urologic and reproductive health conditions
- COVID-19 Impact on Reproduction
- Genetic and phenotypic traits in livestock
- Endometriosis Research and Treatment
University of Missouri
2022-2025
University of Florida
2017-2019
Seminal plasma has conventionally been viewed as a transport and survival medium for mammalian sperm; however, its role now extends beyond this process to actively targeting female tissues. Studies in rodents, swine, humans demonstrate that seminal induces molecular cellular changes within the endometrium or cervix following insemination. Seminal-plasma-induced alterations maternal environment have theorized facilitate embryo development, modulate immunity toward conceptus, potentially...
Understanding how epithelial cells in the female reproductive tract (FRT) differentiate is crucial for health, yet underlying mechanisms remain poorly defined. At birth, FRT epithelium highly malleable, allowing differentiation into various types, but regulatory pathways guiding these early cell fate decisions are unclear. Here, we use neonatal mouse endometrial organoids and assembloid coculture models to investigate innate cellular plasticity external mesenchymal signals influence...
Postnatal development of the uterus involves specification undifferentiated epithelium into uterine-type epithelium. That is regulated by stromal-epithelial interactions as well intrinsic cell-specific transcription factors and gene regulatory networks. This study utilized mouse genetic models Esr1 deletion, endometrial epithelial organoids (EEO), organoid-stromal co-cultures to decipher role in uterine development. Organoids derived from wild-type (WT) mice developed a normal single layer...
Semen induces post-coital inflammation of the endometrium in several species. Post-coital is proposed to alter endometrial environment early pregnancy, mediate embryonic development and modulate maternal immune response pregnancy. In cattle, it common for pregnancies occur absence whole semen due high utilization artificial insemination. Here, we have utilized a cell culture system characterize semen-induced expression inflammatory mediators bovine cells test efficacy transforming growth...
The mechanisms regulating early stages of placentation and trophectoderm differentiation in the ruminant conceptus remain poorly understood. Here we present a model (TE) vitro from outgrowths individual derived embryos. Cell expressed markers mononucleate (MNC) binucleate (BNC) TE cells. percentage BNC ranged 14 to 39% as determined by flow cytometry. Pregnancy-associated glycoproteins (PAGs), produced BNC, were measured culture media on days 35 54. Continuous secretion PAGs was observed...
Postnatal development of the uterus involves specification undifferentiated epithelium into uterine-type epithelium. That is regulated by stromal-epithelial interactions as well intrinsic cell-specific transcription factors and gene regulatory networks. Here, we present a co-culture system to study effects stromal-derived on epithelial cell growth differentiation organoids. First, describe isolation organoid characterization. Second, detail that allows paracrine development. For complete...
Currently, the only measure of sire fertility in bovine is conception rate (SCR), which determined by Day 70 pregnancy diagnosis and not reflective early embryo development. Therefore, this study aimed to establish relationship between SCR In first experiment, 65 sires negative (<−1, n=25), average (−1 1, n=19), high (> +1, n=21) were characterised for their ability produce embryos using an invitro production (IVP) system. For each sire, 100 cumulus–oocyte complexes (COCs)...