A5091068268- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Reproductive System and Pregnancy
- Genetic Mapping and Diversity in Plants and Animals
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Effects and risks of endocrine disrupting chemicals
- Immunotherapy and Immune Responses
- Sperm and Testicular Function
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- CAR-T cell therapy research
- Renal and related cancers
- Estrogen and related hormone effects
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- GDF15 and Related Biomarkers
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
University of Missouri
2023-2025
University of Missouri Health System
2024-2025
Washington State University
2020-2022
The oviduct is the site of fertilization and preimplantation embryo development in mammals. Evidence suggests that gametes alter oviductal gene expression. To delineate adaptive interactions between gamete/embryo, we performed a multi-omics characterization tissues utilizing bulk RNA-sequencing (RNA-seq), single-cell (scRNA-seq), proteomics collected from distal proximal at various stages after mating mice. We observed robust region-specific transcriptional signatures. Specifically, presence...
One of the keys to achieving skin regeneration lies within understanding heterogeneity neonatal fibroblasts, which support regeneration. However, molecular underpinnings regulating cellular states and fates these cells are not fully understood. To investigate this, we performed a parallel multiomics analysis by processing murine for single-cell Assay Transposase-Accessible Chromatin sequencing RNA separately. Our approach revealed that fibroblast clusters could be sorted into papillary...
The oviduct is the site of fertilization and preimplantation embryo development in mammals. Evidence suggests that gametes alter oviductal gene expression. To delineate adaptive interactions between gamete/embryo, we performed a multi-omics characterization tissues utilizing bulk RNA-sequencing (RNA-seq), single-cell (scRNA-seq), proteomics collected from distal proximal at various stages after mating mice. We observed robust region-specific transcriptional signatures. Specifically, presence...
Understanding how epithelial cells in the female reproductive tract (FRT) differentiate is crucial for health, yet underlying mechanisms remain poorly defined. At birth, FRT epithelium highly malleable, allowing differentiation into various types, but regulatory pathways guiding these early cell fate decisions are unclear. Here, we use neonatal mouse endometrial organoids and assembloid coculture models to investigate innate cellular plasticity external mesenchymal signals influence...
Abstract Embryo implantation is a critical event in the establishment of pregnancy, and failure major cause pregnancy loss women. Coordinated, cell‐type specific responses to ovarian steroid hormones, estrogen, progesterone, within endometrium underlie successful embryo establishment. In this study, we utilized glandular epithelium (GE) Cre recombinase mouse line that only active adult ( Prss29 ‐Cre) determine biological role progesterone receptor (PGR) uterine glands during pregnancy....
One of the endogenous estrogens, 17β-estradiol (E2) is a female steroid hormone secreted from ovary. It well established that E2 causes biochemical and histological changes in uterus. However, it not completely understood how regulates oviductal environment vivo. In this study, we assessed effect on each cell type, using an ovariectomized-hormone-replacement mouse model, single-cell RNA-sequencing (scRNA-seq), situ hybridization, cell-type-specific deletion mice. We found type oviduct...
ABSTRACT The oviduct is the site of fertilization and preimplantation embryo development in mammals. Evidence suggests that gametes alter oviductal gene expression. To delineate adaptive interactions between gamete/embryo, we performed a multi-omics characterization tissues utilizing bulk RNA-sequencing (RNA-seq), single-cell (scRNA-seq), proteomics collected from distal proximal at various stages after mating mice. We observed robust region-specific transcriptional signatures. Specifically,...
Abstract Due to the vital roles of macrophages in pathogenesis endometriosis, targeting could be a promising therapeutic direction. Here, we investigated efficacy niclosamide for resolution perturbed microenvironment caused by dysregulated mouse model endometriosis. Single-cell transcriptomic analysis revealed heterogeneity including three intermediate subtypes with sharing characteristics traditional “small” or “large” peritoneal (SPMs and LPMs) cavity. Endometriosis-like lesions (ELL)...
Abstract Ciliary action performs a critical role in the oviduct (Fallopian tube) during pregnancy establishment through sperm and egg transport. The disruption of normal ciliary function affects oocyte pick-up is contributing factor to female infertility. Estrogen an important regulator promotes ciliogenesis several species. Global loss estrogen receptor α (ESR1) leads We have previously shown that ESR1 oviductal epithelial cell layer required for fertility. Here, we assessed on...
The oviduct is the site of fertilization and preimplantation embryo development in mammals. Evidence suggests that gametes alter oviductal gene expression. To delineate adaptive interactions between gamete/embryo, we performed a multi-omics characterization tissues utilizing bulk RNA-sequencing (RNA-seq), single-cell (scRNA-seq), proteomics collected from distal proximal at various stages after mating mice. We observed robust region-specific transcriptional signatures. Specifically, presence...
Abstract Pre‐implantation embryonic development occurs in the oviduct during first few days of pregnancy. The presence oviductal extracellular vesicles (oEVs, also called oviductosomes) is crucial for pre‐implantation vivo as oEVs often contain molecular transmitters such proteins. Therefore, evaluating oEV cargo early pregnancy could provide insights into factors required proper that are missing current vitro embryo culture setting. In this study, we isolated from fluid at estrus and...
The oviduct is the site of fertilization and preimplantation embryo development in mammals. Evidence suggests that gametes alter oviductal gene expression. To delineate adaptive interactions between gamete/embryo, we performed a multi-omics characterization tissues utilizing bulk RNA-sequencing (RNA-seq), single-cell (scRNA-seq), proteomics collected from distal proximal at various stages after mating mice. We observed robust region-specific transcriptional signatures. Specifically, presence...
Abstract Background Exposure to endocrine-disrupting chemicals (EDCs), such as bisphenol A (BPA), disrupts reproduction across generations. Germ cell epigenetic alterations are proposed bridge transgenerational reproductive defects resulting from EDCs. Previously, we have shown that prenatal exposure environmentally relevant doses of BPA or its substitute, BPS, caused transgenerationally maintained impairments associated with neonatal spermatogonial changes in male mice. While germ cells can...
Abstract One of the endogenous estrogens, 17β-estradiol (E 2 ) is a female steroid hormone secreted from ovary. It well established that E causes biochemical and histological changes in uterus. The oviduct response to virtually unknown an vivo environment. In this study, we assessed effect on each oviductal cell type, using ovariectomized-hormone-replacement mouse model, single RNA-sequencing (scRNA-seq), situ hybridization, cell-type-specific deletion mice. We found type responded...
Abstract Due to the vital roles of macrophages in pathogenesis endometriosis, targeting could be a new therapeutic direction. Here, we investigated efficacy niclosamide for resolution perturbed microenvironment caused by dysregulated mouse model endometriosis. Single-cell transcriptomic analysis revealed heterogeneity macrophage subpopulations including three newly identified intermediate subtypes with sharing characteristics traditional “small” or “large” peritoneal (SPMs and LPMs) cavity....