Chlorpyrifos is one of the most used organophosphorus insecticides. It commonly degraded to 3,5,6-trichloro-2-pyridinol (TCP), which water-soluble and toxic. Bacteria can degrade chlorpyrifos TCP, but biodegradation mechanism has not been well-characterized. Recently isolated Cupriavidus nantongensis X1T completely 100 mg/L 20 TCP with half-lives 6 8 h, respectively. We annotated a complete gene cluster responsible for degradation in recently sequenced strain X1T. Two key genes, tcpA fre,...

Chlorpyrifos was one of the most widely used organophosphorus insecticides and neurotoxicity genotoxicity chlorpyrifos to mammals, aquatic organisms other non-target have caused much public concern. Cupriavidus nantongensis X1T, a type strain genus Cupriavidus, is capable efficiently degrading 200 mg/L within 48 h. This ~100 fold faster than Enterobacter B-14, well-studied chlorpyrifos-degrading bacterial strain. Strain X1T can tolerate high concentrations (500 mg/L) over wide range...

A Gram-stain-negative, aerobic, coccoid to small rod-shaped bacterium, designated X1T, was isolated from sludge collected the vicinity of a pesticide manufacturer in Nantong, Jiangsu Province, China. Based on 16S rRNA gene sequence analysis, strain X1T belonged genus Cupriavidus, and most closely related Cupriavidus taiwanensis LMG 19424T (99.1 % similarity) alkaliphilus 26294T (98.9 %). Strain showed similarities 97.2-98.2 with other species Cupriavidus. The major cellular fatty acids were...

Organophosphorus insecticides account for approximately 28% of the global commercial insecticide market, while 40% them are chiral enantiomers. Chiral enantiomers differ largely in their toxicities. Enantiomers that less active or inactive do not offer needed efficacy but pollute environment and cause toxicities to non-target species. Cupriavidus nantongensis X1T, a recently isolated bacterial strain, could degrade S-profenofos 2.3-fold faster than R-profenofos, latter is enantiomer potently...

Cupriavidus nantongensis X1T is a type strain of the genus Cupriavidus, that can degrade eight kinds organophosphorus insecticides (OPs). Conventional genetic manipulations in species are time-consuming, difficult, and hard to control. The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) system has emerged as powerful tool for genome editing applied prokaryotes eukaryotes due its simplicity, efficiency, accuracy. Here, we combined CRISPR/Cas9 with...

Organophosphorus insecticides (OPs) have been widely used to control agricultural pests, which has raised concerns about OP residues in crops and the environment. In this study, we investigated degradation kinetics pathways of 8 OPs by Cupriavidus nantongensis X1T identified enzyme via gene cloning vitro assays. The half-life methyl parathion, triazophos, phoxim was only 5, 9, 43 min, respectively. It 46 fold faster than that triazophos Bacillus sp. TAP-1, a well-studied triazophos-degrader....

The wide use of the insecticide profenofos in crop production has led to serious ecological water problems agricultural fields. With increasing global nuts, a large amount nutshell waste impact on environment. Turning into biochar remove high levels is cost-effective treatment method. In this study, biochars made from are investigated for adsorption aromatic organophosphorus profenofos. was 13-fold higher than stalk removing water. results indicated that by specific. Scanning electron...

The mitochondrial genome structure of a teleostean group is generally considered to be conservative. However, two types gene arrangements have been identified in the mitogenomes Anguilliformes. In this study, we report complete Ariosoma meeki (Anguilliformes (Congridae)). For research, first, and composition were analyzed. As opposed typical arrangement pattern other Anguilliformes species, mitogenome A. has undergone rearrangement. ND6 conjoint tRNA-Glu genes translocated location between...

Text watermarking for Large Language Models (LLMs) has made significant progress in detecting LLM outputs and preventing misuse. Current techniques offer high detectability, minimal impact on text quality, robustness to editing. However, current researches lack investigation into the imperceptibility of services. This is crucial as providers may not want disclose presence watermarks real-world scenarios, it could reduce user willingness use service make more vulnerable attacks. work first...

This paper introduces DiffPuter, an iterative method for missing data imputation that leverages the Expectation-Maximization (EM) algorithm and Diffusion Models. By treating as hidden variables can be updated during model training, we frame task EM problem. During M-step, DiffPuter employs a diffusion to learn joint distribution of both observed currently estimated data. In E-step, re-estimates based on conditional probability given data, utilizing learned in M-step. Starting with initial...

Diazinon, an organophosphorus insecticide, is predominantly removed through photodegradation and biodegradation in the environment. However, can generate diazoxon, a highly toxic oxidation byproduct, while hard to complete mineralize diazinon, showing limitations both methods. In this study, we provided efficient strategy for harmless removal of diazinon by synergistically employing photodegradation. The diazinon-degrading strain X1 was capable completely degrading 200 μM into...

(-)-Epigallocatechin-3-gallate (EGCG) is reported to have benefits for the treatment of Alzheimer's disease by binding with acetylcholinesterase (AChE) enhance cholinergic neurotransmission. Organophosphorus pesticides (OPs) inhibited AChE and damaged nervous system. This study investigated combined effects EGCG OPs on activities in vitro & vivo. The results indicated that significantly reversed inhibition caused OPs. In vitro, reactived three group tubes incubated 110 min, vivo, it...

Autoregressive models are predominant in natural language generation, while their application tabular data remains underexplored. We posit that this can be attributed to two factors: 1) contains heterogeneous type, the autoregressive model is primarily designed discrete-valued data; 2) column permutation-invariant, requiring a generation generate columns arbitrary order. This paper proposes Diffusion-nested (TabDAR) address these issues. To enable methods for continuous columns, TabDAR...