A5060948389- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Escherichia coli research studies
- Trace Elements in Health
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Immune responses and vaccinations
- Cancer Immunotherapy and Biomarkers
- Poxvirus research and outbreaks
- Immune cells in cancer
Brigham and Women's Hospital
2024
Harvard University
2024
Massachusetts General Hospital
2024
Boston VA Research Institute
2024
Oregon Health & Science University
2015-2019
Reed College
2015
Tissue-resident memory (Trm) CD8+ T cells are functionally distinct from their circulating counterparts and potent mediators of host protection against reinfection. Whether local recognition antigen in nonlymphoid tissues during infection can impact the formation Trm populations remains unresolved. Using skin infections with vaccinia virus (VacV)–expressing model antigens, we found that had a profound on formation. Activated trafficked to VacV-infected an inflammation-dependent, but...
Memory CD8+ T cells in the circulation rapidly infiltrate non-lymphoid tissues following infection and provide protective immunity an antigen-specific manner. However, subsequent fate of memory after entering such as skin during a secondary is largely unknown. Furthermore, because expression CD62L often used to identify central (TCM) cell subset, uncoupling physical requirement for CD62L-mediated lymph node homing versus other functional attributes TCM remains unresolved. Here, we show that...
Central memory CD8 + T cells are the major cell subset that traffics into inflamed, nonlymphoid tissues.
Zinc supplements are an effective clinical treatment for infantile diarrheal disease caused by enteric pathogens. Previous studies demonstrated that zinc acts on enteropathogenic Escherichia coli (EPEC) bacteria directly to suppress several virulence-related genes at a concentration can be achieved oral delivery of dietary supplements. Our in vitro showed micromolar induced the envelope stress response and suppressed virulence EPEC, providing possible mechanistic explanation zinc's...
Enteropathogenic Escherichia coli is an important cause of profuse, watery diarrhea in 32infants living developing regions the world. Typical strains EPEC (tEPEC) possess a virulence plasmid, while related clinical isolates that lack EAF plasmid are termed atypical (aEPEC). tEPEC and aEPEC tend to acute versus more 35chronic type infections, respectively. The encodes attachment factor as well regulatory operon, perABC. PerC, poorly understood regulator, was previously shown regulate...
Abstract Tissue-resident memory (TRM) CD8+ T cells are functionally distinct from their circulating counterparts and potent mediators of host protection against re-infection. One largely accepted paradigm is that activated able to freely migrate non-lymphoid tissues differentiate into TRM independent local antigen recognition. In contrast, using skin infections with Vaccinia virus (VacV) expressing model antigens, we found recognition had a profound impact on formation. Activated trafficked...
Abstract CD8+ T cells differentiate into two subpopulations in response to acute viral infection: memory precursor effector (MPECs) and short-lived (SLECs). MPECs SLECs are epigenetically distinct; however, the epigenetic regulators required for formation of these mostly unknown. In this study, we performed an vivo CRISPR screen murine naive identify MPEC SLEC formation, using lymphocytic choriomeningitis virus Armstrong infection model. We identified ATP-dependent chromatin remodeler CHD7...
Abstract Following successful vaccination or pathogen clearance, recently activated CD8+ T cells that survive contraction differentiate into long-lived memory populations and provide host protection against re-infection. We have previously demonstrated acquire the capacity to generate core 2 O-glycans in an antigen-independent manner, a post-translational modification required for binding P- E-selectin. In addition, we shown de novo synthesis of can be regulated by IL-15 signaling this...