Background/aims To examine the incidence, causative microorganisms and in vitro antimicrobial susceptibility resistance profiles of infectious keratitis (IK) Nottingham, UK. Methods A retrospective study all patients who were diagnosed with IK underwent corneal scraping between July 2007 October 2019 (a 12-year period) at a UK tertiary referral centre. Relevant data, including demographic factors, microbiological antibiotic IK, analysed. Results The estimated incidence was 34.7 per 100 000...

Purpose.: To report in vivo confocal microscopic (IVCM) features corneal edema supported by histopathologic correlation. Methods.: This was an observational study with evaluation of diagnostic technology. Twenty patients clinically diagnosed were involved, including 11 Fuchs' endothelial dystrophy (FED). All cases, addition to a control group six normal eyes, examined IVCM before keratoplasty. Four eyes after surgery. Thirteen samples obtained penetrating keratoplasty light and/or electron...

To prospectively study patients presenting with sight-threatening corneal ulcers a view to identify the predisposing factors, causative organisms, clinical signs and treatment outcomes.Prospectively, over 3-year period, all cases serious infective keratitis Queens Medical Hospital in Nottingham, UK, were recruited. Detailed information on aetiology, culture results, & symptoms, given patient's response was collected statistically analysed.One hundred forty-three eyes of 129 enrolled....

Aims To describe clinical and in vivo confocal microscopy (IVCM) features of neuropathic corneal pain (NCP) without clinically visible signs. Methods Prospective, observational study 27 eyes 14 patients who had continuous severe ocular for one or more years, with minimal no surface signs were non-responsive to topical lubricants, steroids and/or ciclosporin. All evaluated using Ocular Surface Disease Index, Oxford grading scale, Schirmer test 1, Cochet Bonnet esthesiometry response...

To highlight the potential risk of herpetic eye disease (HED) reactivation following COVID-19 vaccine.Retrospective analysis all patients who presented with HED within 28 days post-first dose vaccination.Eleven eyes (n = 10 patients) were included. The mean interval between vaccination and ocular symptoms/signs was 12.3 ± 10.3 days. Four (40%) HSV keratitis, six (60%) VZV keratitis (five had concurrent other signs herpes zoster ophthalmicus). Common included multiple scattered...

To determine the refractive outcomes of wavefront-optimized laser in situ keratomileusis (LASIK) treatments, particular to measure corneal densitometry after LASIK using function Pentacam Scheimpflug system.Division Ophthalmology and Visual Sciences, University Nottingham, United Kingdom.Cohort study.Changes postoperative visual acuity, refraction, contrast sensitivity were evaluated treatment. Corneal was with system before LASIK.One year postoperatively, uncorrected distance acuity 6/6 or...

Abstract Purpose To evaluate the corneal sub‐basal nerve plexus in patients presenting with hypoesthesia following surgery for trigeminal neuralgia. Methods Twenty‐one who had unilateral medically uncontrolled neuralgia and underwent ipsilateral from 2006 to 2012 were included. Of these, 10 microvascular decompression ( MVD group) 11 balloon compression of ganglion BC group). Slit lamp examination, Cochet–Bonnet aesthesiometery vivo confocal microscopy carried out on both eyes each patient....

The authors have previously reported the presence of antimicrobial peptides human beta defensin (hBD) 3 and hBD9 on ocular surface (OS). These play an important role in infection inflammation. In present study, studied gene expression levels hBD3 healthy subjects during after healing infectious keratitis.Human OS specimens were obtained by impression cytology from controls patients with Acanthamoeba Gram-negative -positive bacterial keratitis (BK), both active healing. determined using...

To analyse the efficacy of paired arcuate incisions and compression sutures technique in management post penetrating keratoplasty (PK) astigmatism.A incision with procedure was used to treat 26 eyes post-PK astigmatism ranging from 6.00 16.50 dioptres (D). The were placed at 7.0 mm optical zone inside graft-host junction a depth 80% corneal thickness. A 45° arc length planned for preoperative between 6D 9D, 60° >9D. At 3 months, topography refraction performed suture(s) removed if indicated....

Aims To demonstrate and characterise distinct subepithelial compact nerve endings (CNE) at the human corneoscleral limbus. Methods Ten fresh donor discs (mean age, 67 years) 26 organ-cultured rims 59 were studied. All samples subjected to enzyme histochemical staining related endogenous acetylcholinesterase present in tissue H&E staining. Whole-mount en face imaging with NanoZoomer digital pathology microscope serial cross-section light undertaken. Results Nerves entering limbus...

Aims To describe the in vivo confocal microscopy (IVCM) features of human limbal nerve corpuscles (LNCs) and correlate these with histological features. Methods We examined 40 eyes 29 healthy living subjects (17 female, 12 male; mean age=47.6) by IVCM. Four quadrants were scanned through all epithelial layers stroma to identify LNCs associated nerves. Ten fresh normal corneoscleral discs from five deceased patients a age 67 years 17 eye-bank rims 57.6 stained as whole mounts...

To study the morphology of human corneal nerves in eye bank organ-cultured corneas and grafts post-transplantation.Thirty-seven were divided into: Group-A, anterior 300-400 μm 20 used for Descemets stripping endothelial keratoplasty, Group-B, 17 full-thickness unsuitable transplantation. Corneas whole mounts stained using acetylcholinesterase technique examined by NanoZoomer digital pathology microscope. Central sub-Bowman's stromal sub-basal nerve plexus including perforation sites terminal...