A5024367204- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Nuclear Structure and Function
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
UCSF Helen Diller Family Comprehensive Cancer Center
2024
University of California, San Francisco
2023-2024
Heidelberg University
2017-2023
The RNA exosome captured in action exosome, a major degradation machine, processes ribosomal (rRNA) precursors and is directly coupled to the protein synthesis ribosome. Using cryo–electron microscopy, Schuller et al. investigated structure of precursor large subunit from yeast with unprocessed rRNA complex exosome. captures snapshot two molecular machines transiently interacting explains how acts on an authentic physiological substrate remodels during ribosome maturation. Science , this issue p. 219
Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by exonucleolytic trimming at both new sites generate mature 5.8S and 25S rRNA. We reconstituted the 7S→5.8S branch within ITS2 using purified exosome its nuclear cofactors. find that Rrp44's ribonuclease activities are required for initial shortening hand over exonuclease Rrp6. During in...
Abstract The rixosome defined in Schizosaccharomyces pombe and humans performs diverse roles pre‐ribosomal RNA processing gene silencing. Here, we isolate describe the conserved from Chaetomium thermophilum , which consists of two sub‐modules, sphere‐like Rix1‐Ipi3‐Ipi1 butterfly‐like Las1‐Grc3 complex, connected by a flexible linker. Rix1 complex utilizes Sda1 as landing platform on nucleoplasmic pre‐60S particles to wedge between 5S rRNA tip L1‐stalk, thereby facilitating 180° rotation...
Ribosomes are emerging as direct regulators of gene expression, with ribosome-associated proteins (RAPs) allowing ribosomes to modulate translational control. However, a lack technologies enrich RAPs across many sample types has prevented systematic analysis RAP number, dynamics, and functions. Here, we have developed label-free methodology called RAPIDASH from any sample. We applied mouse embryonic tissues identified hundreds potential RAPs, including DHX30 LLPH, two forebrain important for...