A5032072916- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
Duke University
2025
Center for Cancer Research
2020-2022
National Cancer Institute
2020-2022
National Institutes of Health
2022
Abstract In the presence of physiological monovalent cations, thousands RNA G-rich sequences can form parallel G-quadruplexes (G4s) unless RNA-binding proteins inhibit, destabilize, or resolve formation such secondary structures. Here, we have used a disease-relevant model system to investigate biophysical properties protein HNRNPH1’s interaction with sequences. We demonstrate importance two EWSR1-exon 8 regions in mediating exclusion this exon from oncogenic EWS-FLI1 transcripts expressed...
G protein–coupled receptors (GPCRs) modulate various physiological functions by rewiring cellular gene expression in response to extracellular signals. Control of GPCRs has been studied almost exclusively at the transcriptional level, neglecting an extensive amount regulation that takes place translationally. Hence, little is known about nature and mechanisms gene-specific posttranscriptional downstream receptor activation. Here, we apply unbiased multiomics approach delineate translational...
Abstract The year 2021 marks the 20th anniversary of first publications reporting discovery gene silencing mechanism, RNA interference (RNAi) in mammalian cells. Along with many studies that delineated proteins and substrates form RNAi pathway, this finding changed our understanding posttranscriptional regulation expression. Furthermore, development methods exploited pathway began technological revolution eventually enabled interrogation function—from a single to whole genome—in only few...
ABSTRACT In the presence of physiological monovalent cations, thousands RNA G-rich sequences can form parallel G-quadruplexes (G4s) unless RNA-binding proteins inhibit, destabilize, or resolve formation such secondary structures. Here, we have used a disease-relevant model system to investigate biophysical properties protein HNRNPH1’s interaction with sequences. We demonstrate importance two EWSR1 -exon 8 regions in mediating exclusion this exon from oncogenic EWS-FLI1 transcripts expressed...