A5006081547- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- SARS-CoV-2 detection and testing
- 3D Printing in Biomedical Research
- Nanopore and Nanochannel Transport Studies
- SARS-CoV-2 and COVID-19 Research
University of Kansas
2020-2022
We report a microfluidic assay to select active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles (VPs), which were defined as intact with an accessible angiotensin-converting enzyme receptor binding domain (RBD) on the spike (S) protein, from clinical samples. Affinity selection of SARS-CoV-2 was carried out using injection molded chips, allow for high-scale production accommodate large-scale screening. The contained surface-bound aptamer directed against virus's...
Liquid biopsies are becoming popular for managing a variety of diseases due to the minimally invasive nature their acquisition, thus potentially providing better outcomes patients. Circulating tumor cells (CTCs) among many different biomarkers secured from liquid biopsy, and number efficient platforms isolation enrichment blood have been reported. However, these require manual sample handling, which can generate difficulties when translating CTC assays into clinic potential loss,...
We detail a heterobifunctional, 7-aminocoumarin photocleavable (PC) linker with unique properties to covalently attach Abs surfaces and subsequently release them visible light (400–450 nm).
We report an in-plane extended nanopore Coulter counter (XnCC) chip fabricated in a thermoplastic via imprinting. The fabrication of the sensor utilized both photolithography and focused ion beam milling to make microfluidic network pore sensor, respectively, Si from which UV resin stamps were generated followed by thermal imprinting produce final device appropriate plastic (cyclic olefin polymer, COP). As example utility this we enumerated SARS-CoV-2 viral particles (VPs) affinity-selected...