A5030335115- Polyomavirus and related diseases
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Antenna Design and Analysis
- Tumors and Oncological Cases
- Full-Duplex Wireless Communications
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- Gut microbiota and health
- Immunotherapy and Immune Responses
- Parvovirus B19 Infection Studies
- Microtubule and mitosis dynamics
- Renal and related cancers
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Synthesis and biological activity
- Inflammasome and immune disorders
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Pancreatic function and diabetes
- Viral Infections and Immunology Research
- DNA Repair Mechanisms
Stanford University
2003-2025
Novartis (United States)
2015-2023
Palo Alto University
2023
DuPont (United States)
2019-2020
Precision for Medicine (United States)
2019-2020
Baylor College of Medicine
1998-2019
Michigan State University
2006-2010
University of California, San Francisco
2004-2008
California Institute for Regenerative Medicine
2008
San Francisco VA Medical Center
2004
In the Canakinumab Anti-inflammatory Thrombosis Outcomes Study (CANTOS), inhibition of IL1β inflammatory pathway by canakinumab has been shown to significantly reduce lung cancer incidence and mortality. Here we performed molecular characterization CANTOS patients who developed during study, including circulating tumor DNA (ctDNA) soluble biomarker analysis. Catalogue Somatic Mutations in Cancer (COSMIC) database ctDNA mutations were detected 65% (46/71) with cancer, 51% (36/71) having...
Abstract Background Mammalian cells have been reported to a p53-dependent tetraploidy checkpoint that blocks cell cycle progression in G1 response failure of division. In most cases where the has observed division was perturbed by anti-cytoskeleton drug treatments. However, other evidence argues against existence checkpoint. Cells failed divide differ from normal having two nuclei, centrosomes, decreased surface volume ratio, and undergone an abortive cytokinesis. We tested each these...
Abstract High levels of IL1β can result in chronic inflammation, which turn promote tumor growth and metastasis. Inhibition could therefore be a promising therapeutic option the treatment cancer. Here, effects blockade induced by mAbs canakinumab gevokizumab were evaluated alone or combination with docetaxel, anti–programmed cell death protein 1 (anti–PD-1), anti-VEGFα, anti-TGFβ syngeneic humanized mouse models cancers different origin. Canakinumab did not show notable efficacy as...
The Pit1-Oct1-Unc86 domain (POU domain) transcription factor Brn3a controls sensory neuron survival by regulating the expression of Trk receptors and members Bcl-2 family. Loss leads to a dramatic increase in apoptosis severe loss neurons ganglia. Although recent evidence suggests that Brn3a-mediated can be modified additional cofactors, exact mechanisms are not known. Here, we report homeodomain interacting protein kinase 2 (HIPK2) is pro-apoptotic transcriptional cofactor suppresses gene...
Molecular monitoring of chronic myeloid leukemia patients using robust BCR-ABL1 tests standardized to the International Scale (IS) is key proper disease management, especially when treatment cessation considered. Most laboratories currently use a time-consuming sample exchange process with reference for IS calibration. A World Health Organization (WHO) panel was developed (MR(1)-MR(4)), but access material limited. In this study, we describe development first cell-based secondary that...
The transforming potential and oncogenicity of a simian virus 40 (SV40) mutant affecting T-antigen (T-ag), SV40(cT)-3, was examined in an effort to dissect T-ag functions transformation. SV40(cT)-3 has point mutation at nucleotide 4434 that abolishes the transport nucleus but does not affect its association with cell surface. Transfection-transformation assays were performed primary cells established lines mouse rat origin. efficiency transformation for by comparable wild-type SV40,...
Several methods allow reprogramming of differentiated somatic cells to embryonic stem cell-like cells. However, the process remains inefficient and underlying molecular mechanisms are poorly understood. Here, we report optimization cell fusion with in order provide an efficient, quantitative assay screen for factors that facilitate reprogramming. Following optimization, achieved a efficiency 15–590 fold higher than previous protocols. This allowed observation cellular events during process....
Traditional microbiological enumeration methods have long been employed as the standard evaluation procedure for probiotic microorganisms. These are labor intensive, long-time to results and inherently a high degree of variability – up 35%. As clinical microbiome science continues grow develop, it is increasingly important that researchers thoroughly define deliver targeted dose. Furthermore, establish quality commercial products, same dosage level must be administered consumers. An ISO...
ABSTRACT Simian virus 40 (SV40) DNAs in brain tissue and peripheral blood mononuclear cells (PBMCs) of eight simian immunodeficiency virus-infected rhesus monkeys with SV40 disease were analyzed. We report the detection, cloning, identification five new strains following a quadruple testing-verification strategy. genomes archetypal regulatory regions (containing duplication within G/C-rich region segment single 72-bp enhancer element) recovered from seven animal brains, two tissues which...
Recent studies have detected simian virus 40 (SV40) DNA in certain human tumors and normal tissues. The significance of infections by SV40, which was first discovered as a contaminant poliovirus vaccines used between 1955 1963, remains unknown. occurrence SV40 unselected hospitalized children evaluated. Polymerase chain reaction sequence analyses were done on archival tissue specimens from patients positive for neutralizing antibody. identified samples 4 20 (1 Wilms' tumor, 3 transplanted...
Mucoid exopolysaccharide (MEP) obtained from Pseudomonas aeruginosa 579 was suspended in 10 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) pH 7.2 containing 0.1-10.0 of CaCl2.2H2O or MgCl2.4H2O. MEP treated with HEPES < 5.0 the Ca2+ Mg2+ salts remained soluble and bound tobramycin an equilibrium dialysis bioassay. 10.0 did not bind tobramycin. Five Ca(2+)-treated precipitated but Mg(2+)-treated not. biofilms formed using a defined growth medium having 1 were for h +/-...
Effects of polyomavirus SV40 microRNA on pathogenesis viral infections in vivo are not known. Syrian golden hamsters the small animal model for studies SV40. We report here effects and influence structure regulatory region dynamics DNA levels vivo. Outbred young adult were inoculated by intracardiac route with 1×107 plaque-forming units four different variants Infected animals sacrificed from 3 to 270 days postinfection loads tissues determined quantitative real-time polymerase chain...
The transforming potential and oncogenicity of a simian virus 40 (SV40) mutant affecting T-antigen (T-ag), SV40(cT)-3, was examined in an effort to dissect T-ag functions transformation. SV40(cT)-3 has point mutation at nucleotide 4434 that abolishes the transport nucleus but does not affect its association with cell surface. Transfection-transformation assays were performed primary cells established lines mouse rat origin. efficiency transformation for by comparable wild-type SV40,...
Improvements offered by viability droplet digital PCR (v-ddPCR) include increased precision, specificity and decreased time to results making for an attractive alternative method traditional plate count enumeration of probiotic products. A major hurdle faced in v-ddPCR, however, is distinguishing between live dead cells. The objective this study was evaluate a combination PMA EMA (PE51) treatment freeze-dried powders. Lactobacillus acidophilus La-14 Bifidobacterium animalis subsp. lactis...
Abstract Background For the advancement of cancer research, collection tissue specimens from drug‐resistant tumors after targeted therapy is crucial. Although patients with lung are often provided therapy, post‐therapy not routinely collected due to risks collection, limiting study resistance mechanisms. Posthumous rapid donation (RTD) an expedient process that provides opportunity understand treatment‐resistant cancers. Methods Consent participate in thoracic RTD protocol was obtained...
A phylogenetic analysis of 14 complete simian virus 40 (SV40) genomes was conducted in order to determine strain relatedness and the extent genetic variation. This included infectious isolates recovered between 1960 1999 from primary cultures monkey kidney cells, contaminated poliovaccines an adenovirus seed stock, human malignancies, transformed cells. Maximum-parsimony distance methods revealed distinct SV40 clades. However, no clear patterns association genotype viral source were...