A5082191409- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Nanoparticle-Based Drug Delivery
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Nanoplatforms for cancer theranostics
- Conducting polymers and applications
- Molecular Junctions and Nanostructures
- Supramolecular Chemistry and Complexes
- Hydrogels: synthesis, properties, applications
- Immunotherapy and Immune Responses
- Photochromic and Fluorescence Chemistry
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Zebrafish Biomedical Research Applications
- Advanced Drug Delivery Systems
- Calcium Carbonate Crystallization and Inhibition
- Crystallization and Solubility Studies
- Advancements in Transdermal Drug Delivery
- Polymer Surface Interaction Studies
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
Leiden University
2016-2025
Huazhong University of Science and Technology
2021
Max-Planck-Institut für Nachhaltige Materialien
2015
University of Bristol
2014
Center for Advanced Biomaterials for Healthcare
2014
Italian Institute of Technology
2014
University of Münster
2009-2012
Institute of Chemistry
2012
CeNTech
2012
Centre for Human Drug Research
2012
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low efficiency due limited endosomal escape and/or degradation lysosomes. Here, we report new method for direct drug into cytosol live vitro and vivo utilizing targeted membrane fusion between liposomes cells. A pair complementary coiled-coil lipopeptides was embedded lipid bilayer cell membranes respectively, with concomitant release liposome...
Up to 99% of systemically administered nanoparticles are cleared through the liver. Within liver, most thought be sequestered by macrophages (Kupffer cells), although significant nanoparticle interactions with other hepatic cells have also been observed. To achieve effective cell-specific targeting drugs encapsulation, improved mechanistic understanding nanoparticle–liver is required. Here, we show caudal vein embryonic zebrafish (Danio rerio) can used as a model for assessing mammalian...
Antigen binding by serum Ig-M (IgM) protects against microbial infections and helps to prevent autoimmunity, but causes life-threatening diseases when mistargeted. How antigen-bound IgM activates complement-immune responses remains unclear. We present cryoelectron tomography structures of IgM, C1, C4b complexes formed on antigen-bearing lipid membranes normal human at 4 °C. The IgM-C1-C4b revealed product release as the temperature-limiting step in complement activation. Both hexamers...
Lipid nanoparticles (LNPs) are the leading nonviral technologies for delivery of exogenous RNA to target cells in vivo. As systemic platforms, these exemplified by Onpattro, an approved LNP-based interference therapy, administered intravenously and targeted parenchymal liver cells. The discovery systemically LNP capable preferential beyond hepatocytes has, however, proven more challenging. Here, preceded comprehensive mechanistic understanding vivo nanoparticle biodistribution bodily...
Abstract Self-assembling molecular drugs combine the easy preparation typical of small-molecule chemotherapy and tumour-targeting properties drug–nanoparticle conjugates. However, they require a supramolecular interaction that survives complex environment living animal. Here we report metallophilic between cyclometalated palladium complexes generates nanostructures in mice have long circulation time (over 12 h) efficient tumour accumulation rate (up to 10.2% injected dose per gram) skin...
Tumors growing in metabolically challenged environments, such as glioblastoma the brain, are particularly reliant on crosstalk with their tumor microenvironment (TME) to satisfy high energetic needs. To study intricacies of this metabolic interplay, we interrogated heterogeneity TME using single-cell and multi-omics analyses identified rewired tumor-associated macrophage (TAM) subpopulations pro-tumorigenic properties. These TAM subsets, termed lipid-laden macrophages (LLMs) reflect...
Abstract Efficient cytosolic delivery of RNA molecules remains a formidable barrier for therapeutic strategies. Lipid nanoparticles (LNPs) serve as state-of-the-art carriers that can deliver intracellularly, exemplified by the recent implementation several vaccines against SARS-CoV-2. Using bottom-up rational design approach, we assemble LNPs contain programmable lipid phases encapsulating small interfering (siRNA). A combination cryogenic transmission electron microscopy, tomography and...
Using the inclusion complex of trans azobenzene and cyclodextrin as a photo-switchable crosslinker, dextran based photo-responsive hydrogel system has been constructed employed for light controlled protein release system.
Organic conducting polymers can be synthesized inside the pores of a track-etch membrane, and resulting hollow tubules are shown to have enhanced electrical properties compared their corresponding bulk materials. The polymerization monomers (e.g., pyrrole, thiophenes) confined space these pores, combined with electrostatic interaction, ensures alignment organic on interior, leading higher conductivity. application tubes in development amperometric glucose sensors is discussed. Due special...
A pH sensitive carrier is obtained by coating a cyclodextrin vesicle with an adamantane-terminated octapeptide through the formation of inclusion complex. Upon lowering from 7.4 to 5.0, peptide beta-sheets on surface induces transition bilayer sphere fiber. This fully reversible and repeatable. The vesicles release their cargo upon fiber formation.
Let's get together: A minimal model system was developed to mimic the SNARE-protein-mediated fusion of biological membranes (see picture). Fusion between two populations liposomes is controlled by a pair complementary lipidated oligopeptides that form noncovalent coiled-coil complexes and thereby force into close proximity promote fusion. The displays key characteristics in vivo events.
Folic acid-modified mesoporous silica nanoparticles are designed for cellular and nuclear targeted drug delivery. Camptothecin is efficiently delivered to human cancer cells resulting in apoptosis without premature leakage. The folic acid moiety an integral part of a rotaxane structure, which locks the molecules pores, preventing release. Upon folate receptor-mediated cell uptake, esterases cleave valves camptothecin released, apoptosis. As service our authors readers, this journal provides...
In this study, we investigated the potential of intradermal delivery nanoparticulate vaccines to modulate immune response protein antigen using hollow microneedles. Four types nanoparticles covering a broad range physiochemical parameters, namely poly (lactic-co-glycolic) (PLGA) nanoparticles, liposomes, mesoporous silica (MSNs) and gelatin (GNPs) were compared. The developed loaded with model (ovalbumin (OVA)) without an adjuvant (poly(I:C)), followed by characterization size, zeta...
Mesoporous silica nanoparticles (MSNs) have been explored extensively as solid supports for proteins in biological and medical applications. Small (<200 nm) MSNs with ordered large pores (>5 nm), capable of encapsulating therapeutic small molecules suitable delivery applications vivo, are rare however. Here we present small, elongated, cuboidal, average dimensions 90 × 43 nm that possess disk-shaped cavities, stacked on top each other, which run parallel to the short axis particle. Amine...
Complementary coiled coil forming lipidated peptides embedded in liposomal membranes are able to induce rapid, controlled, and targeted membrane fusion. Traditionally, such fusogenic liposomes prepared by mixing lipids organic solvent (e.g., chloroform). Here we situ, i.e., addition of a peptide solution plain liposomes. As the lipid anchor is vital for correct insertion into membranes, small library was designed which structure varied. The fusogenicity screened using content assays showing...
Glioblastoma (GBM) is a devastating primary brain tumor resistant to conventional therapies. A major obstacle GBM treatment the blood-brain barrier (BBB), or blood-glioma barrier, which prevents transport of systemically administered (chemotherapeutic) drugs into tumor. This study reports design dodecamer peptide (G23)-functionalized polydopamine (pD)-coated curcumin-loaded zein nanoparticles (CUR-ZpD-G23 NPs) that efficiently traversed BBB, and delivered curcumin glioblastoma cells. The NPs...
Surface charge plays a fundamental role in determining the fate of nanoparticle, and any encapsulated contents, vivo. Herein, we describe, visualise real time, light-triggered switching liposome surface charge, from neutral to cationic, situ vivo (embryonic zebrafish). Prior light activation, intravenously administered liposomes, composed just two lipid reagents, freely circulate successfully evade innate immune cells present fish. Upon irradiation switching, however, liposomes rapidly...