A5067668834- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Head and Neck Cancer Studies
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- CRISPR and Genetic Engineering
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Nanopore and Nanochannel Transport Studies
- Lymphoma Diagnosis and Treatment
University Medical Center Utrecht
2016-2023
Utrecht University
2020-2023
Heidelberg University
2020
University Hospital Heidelberg
2020
National Medical Research Center of Dentistry and Maxillofacial Surgery
2016
During posttreatment surveillance of head and neck cancer patients, imaging is insufficiently accurate for the early detection relapsing disease. Free circulating tumor DNA (ctDNA) may serve as a novel biomarker monitoring burden during these patients. In this exploratory study, we investigated whether low level ctDNA in plasma patients can be detected using Droplet Digital PCR (ddPCR).TP53 mutations were determined surgically resected primary samples from six with high stage (II-IV),...
Abstract In current molecular cancer diagnostics, using blood samples of patients for the detection genetic alterations in plasma (cell‐free) circulating tumor DNA (ct ) is an emerging practice. Since ct levels are low, highly sensitive Droplet Digital PCR (dd can be used detecting rare mutational targets. order to perform dd on samples, a standardized procedure processing and analyzing necessary facilitate implementation into clinical Therefore, we assessed technical sample workup samples....
Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive diagnostics and monitoring. However, detecting ctDNA is challenging, much fewer than 5% the cell-free blood typically originates from tumor. To detect lowly abundant molecules based on somatic variants, extremely sequencing methods are required. Here, we describe new technique, CyclomicsSeq, which Oxford Nanopore concatenated copies single molecule. Consensus...
// Joost H. van Ginkel 1, 2 , Wendy W.J. de Leng Remco Bree 3 Robert J.J. Es Stefan M. Willems 1 Department of Oral and Maxillofacial Surgery, University Medical Center Utrecht, The Netherlands Pathology, Head Neck Surgical Oncology, Correspondence to: Willems, email: s.m.willems-4@umcutrecht.nl Keywords : head neck cancer, mutations, next-generation sequencing, TP53 diagnostic biomarkers Received: May 20, 2016 Accepted: July 28, Published: August 11, ABSTRACT squamous cell carcinomas...
The prognosis of head and neck squamous cell carcinoma (HNSCC) is largely based on disease stage. Despite improvements in treatment, recurrence rates are still considered high. Currently, progression or regression after curative treatment monitored by clinical evaluation combined with flexible endoscopy and/or imaging. However, specificity imaging low due to the posttreatment effects. Detection circulating tumor DNA (ctDNA) from blood samples HNSCC patients a minimally invasive technique...
The prognosis of head and neck squamous cell carcinoma (HNSCC) is largely based on disease stage. Despite improvements in treatment, recurrence rates are still considered high. Currently, progression or regression after curative treatment monitored by clinical evaluation combined with flexible endoscopy and/or imaging.
ABSTRACT Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive diagnostics and monitoring. However, detecting ctDNA is challenging, much fewer than 5% the cell-free blood typically originates from tumor. To detect lowly abundant molecules based on somatic variants, extremely sequencing methods are required. Here, we describe new technique, CyclomicsSeq, which Oxford Nanopore concatenated copies single molecule....