A5058802759- Extracellular vesicles in disease
- Biochemical and Structural Characterization
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Neurological Disorders and Treatments
- Antimicrobial Peptides and Activities
- Neurological Disease Mechanisms and Treatments
- Antioxidant Activity and Oxidative Stress
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Biochemical Analysis and Sensing Techniques
- Peptidase Inhibition and Analysis
- Oral microbiology and periodontitis research
- Sepsis Diagnosis and Treatment
- Mass Spectrometry Techniques and Applications
- Dental Health and Care Utilization
- Edible Oils Quality and Analysis
- Essential Oils and Antimicrobial Activity
- Protein purification and stability
- S100 Proteins and Annexins
Biomedical Research Institute of Lleida
2022-2025
Universitat de Lleida
2009-2025
Hospital Universitari Arnau de Vilanova
2022-2025
Nanyang Technological University
2013-2023
IMDEA Food
2019-2023
Madrid Institute for Advanced Studies
2019-2023
Universidad Autónoma de Madrid
2019-2023
Instituto de Salud Carlos III
2021-2022
Digital Proteomics (United States)
2021
Tufts University
2012
Background: Poor oral health and periodontitis have been epidemiologically linked to cognitive decline mild impairment (MCI) in older adults. However, specific metrics directly linking these clinical signs are exceedingly limited. Methods: To address this gap develop novel tools help clinicians identify individuals at risk of decline, we established the PerioMind Colombia Cohort, comprising elderly Colombian subjects who underwent comprehensive neurocognitive periodontal evaluations....
Among procyanidins (PC), monomers, such as catechin and epicatechin, have been widely studied, whereas dimer trimer oligomers received much less attention, despite their abundance in our diet. Recent studies showed that dimers trimers could be important determining the biological effects of procyanidin-rich food, understanding bioavailability metabolism is fundamental. The purpose present work to study stability PC under digestion conditions, by using a combination vitro vivo models....
Abstract Scope The distribution and accumulation of olive oil phenolic compounds in the body are topics lacked information. aim this study was to evaluate bioavailability, metabolism from cake. Methods results were examined by UPLC‐MS/MS after an acute intake a extract cake, analyzing plasma tissues (heart, brain, liver, kidney, spleen, testicle thymus) 1, 2 4 h ingestion using Wistar rats as vivo model. showed wide their metabolites tissues, with main detoxification route through kidneys....
Abstract Extracellular vesicles (EVs) such as exosomes and microvesicles mediate intercellular communication regulate a diverse range of crucial biological processes. Host cells that are damaged, infected or transformed release biomarker-containing EVs into the peripheral circulation, where they can be readily accessed for use in diagnostic prognostic testing. However, current methods EV isolation from blood plasma complex often require relatively large sample volumes, hence inefficient...
Asparaginyl endopeptidases (AEPs) are cysteine proteases which break Asx (Asn/Asp)–Xaa bonds in acidic conditions. Despite sharing a conserved overall structure with AEPs, certain plant enzymes such as butelase 1 act peptide asparaginyl ligase (PAL) and catalyze Asx–Xaa bond formation near-neutral PALs also serve macrocyclases the biosynthesis of cyclic peptides. Here, we address question how PAL can function rather than protease. Based on sequence homology 1, identified AEPs from...
Significance ARE ABC-F genes have been found in numerous pathogen genomes and multi-drug resistance conferring plasmids. Further transmission will challenge the clinical use of many antibiotics. The development improved ribosome-targeting therapeutics relies on elucidation mechanisms. Characterization MsrE protein bound to bacterial ribosome is first its kind for members. Together with biochemical data, it sheds light protection mechanism by domain linker-mediated conformational change...
Extracellular vesicles (EVs) act as key mediators of intercellular communication and are secreted taken up by all cell types in the central nervous system (CNS). While detailed study EV-based signaling is likely to significantly advance our understanding human neurobiology, technical challenges isolating EVs from CNS tissues have limited their characterization using 'omics' technologies. We therefore developed a new Protein Organic Solvent Precipitation (PROSPR) method that can efficiently...
Abstract Background The contributions of brain intercellular communication mechanisms, specifically extracellular vesicles (EV), to the progression Alzheimer’s disease (AD) remain poorly understood. Methods Here, we investigated role(s) EV in progressive course AD through unbiased proteome-wide analyses temporal lobe-derived and proteome-label quantitation complementary remaining portions. Furthermore, relevant proteins identified were further screened by multiple reaction monitoring....
Dementia risk in women is higher than men, but the molecular neuropathology of this gender difference remains poorly defined. In study, we used unbiased, discovery-driven quantitative proteomics to assess basis influences on Alzheimer's disease with cerebrovascular (AD + CVD).We detected modulation several redox proteins temporal lobe AD CVD subjects, and observed sex-specific alterations white matter (WM) mitochondria proteomes female patients. Functional proteomic analysis brain tissues...
Increased oxidative stress (OS) and the disruption of equilibrium between production reactive oxygen species antioxidants are key molecular features unhealthy aging. OS results in formation posttranslational modifications (PTMs), some which involve cysteine (Cys) residues aging proteomes, specifically, trioxidized Cys (t-Cys), leads to permanent protein damage. Recent findings rodents have uncovered that irregular regulation t-Cys proteome disrupts homeostatic phosphorylation signaling,...
Abstract Cyclotides are plant cyclic cysteine-rich peptides (CRPs). The nature is reported to be gene-determined with a precursor containing cyclization-competent domain which contains an essential C-terminal Asn/Asp (Asx) processing signal recognized by cyclase. Linear forms of cyclotides rare and likely uncyclizable because they lack this Asx (uncyclotide). Here we show that in the cyclotide-producing Clitoria ternatea , both acyclic products, collectively named cliotides, can bioprocessed...
Vascular factors that reduce blood flow to the brain are involved in apparition and progression of dementia. We hypothesized cerebral hypoperfusion (CH) might alter molecular compositions intercellular communication mechanisms while affecting neurovascular unit preclinical clinical human dementias. To test hypothesis, mice were subjected bilateral common carotid stenosis (BCAS) brain-derived circulating extracellular vesicles (EVs) assessed. Murine vesicle profiles then analyzed parallel...
Procyanidins are present in a wide range of dietary foods and their metabolism is well known. Nevertheless, the biological target distribution topics lacking information. The purpose work was to study procyanidins metabolites rat plasma different tissues, such as liver, brain, lung, kidney, intestine, testicle, spleen, heart thymus, after 2 h an acute intake hazelnut extract rich (5 g kg−1 body weight). interest instead repeated low doses from daily ingestion achieve concentration tissues...
Brain degenerative protein modifications (DPMs) are associated with the apparition and progression of dementia, at same time, Alzheimer's disease cerebrovascular (AD + CVD) is most prevalent form dementia in elder population. Thus, understanding role(s) brain DPMs this subtype may provide novel insight on pathogenesis aid development diagnostic therapeutic tools. Two essential known to promote inflammation several human diseases ureido (uDPMs) arginine citrullination lysine carbamylation,...
Butelase-1 is a peptide asparaginyl ligase (PAL) that efficiently catalyzes bond formation after Asn/Asp (Asx), whereas its homologue butelase-2 an endopeptidase (AEP) the reverse reaction, hydrolyzing Asx-peptide bonds. Since PALs and AEPs share essentially similar overall structures, we surmised S2 S1′ substrate-binding pockets immediately flanking catalytic S1 site constitute major activity determinants (LADs) control directionality of these enzymes. Here, report successful conversion...