A5021941387- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Biotechnology and Related Fields
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Genetically Modified Organisms Research
- Sugarcane Cultivation and Processing
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Congenital heart defects research
- RNA modifications and cancer
- Multimedia Communication and Technology
- Research Data Management Practices
- Video Analysis and Summarization
- Plant Pathogens and Resistance
- Scientific Computing and Data Management
- Bacteriophages and microbial interactions
- Muscle Physiology and Disorders
- Bacterial Genetics and Biotechnology
- Cocoa and Sweet Potato Agronomy
Bielefeld University
2020-2023
Heart and Diabetes Center North Rhine-Westphalia
2020-2022
Simon Fraser University
2022
University Hospitals of the Ruhr-University of Bochum
2021
Ruhr University Bochum
2021
LWL-Universitätsklinikum Bochum
2021
Heterozygous truncating variants in TTN (TTNtv), the gene coding for titin, cause dilated cardiomyopathy (DCM), but underlying pathomechanisms are unclear and disease management remains uncertain. Truncated titin proteins have not yet been considered as a contributor to development. Here, we studied myocardial tissues from nonfailing donor hearts 113 patients with end-stage DCM expression identified TTNtv 22 (19.5%). We directly demonstrate haploinsufficiency TTNtv-DCM absence of...
The 'big data' revolution has enabled novel types of analyses in the life sciences, facilitated by public sharing and reuse datasets. Here, we review prodigious potential reusing publicly available datasets associated challenges, limitations risks. Possible solutions to issues research integrity considerations are also discussed. Due prominence, abundance wide distribution sequencing data, focus on sequence We define 'successful reuse' as use previously published data enable scientific...
A major cause of heart failure is cardiomyopathies, with dilated cardiomyopathy (DCM) as the most common form. Over 40 genes are linked to DCM, among them TTN and RBM20. Next Generation Sequencing in clinical DCM cohorts revealed truncating variants (TTNtv), accounting for up 25% familial cases. Mutations cardiac splicing factor RNA binding motif protein 20 (RBM20) also known be associated severe cardiomyopathies. one RBM20 targets. Most pathogenic mutations localized highly conserved...
Abstract Background As the major source of sugar in moderate climates, sugar-producing beets ( Beta vulgaris subsp. ) have a high economic value. However, low genetic diversity within cultivated requires introduction new traits, for example to increase their tolerance and resistance attributes – traits that often reside crop wild relatives. For this, information beet relatives phylogenetic placements each other are crucial. To answer this need, we sequenced assembled complete plastome...
Abstract Most crop plants, including sugar beet ( Beta vulgaris subsp. ), suffer from domestication bottlenecks and low genetic diversity caused by extensive selection for few traits. However, wild relatives (CWRs) harbour useful traits relevant improvement, enhanced adaptation to biotic abiotic stresses. Especially polyploids are interesting an evolutionary perspective as genes undergo reorganisation after the polyploidisation event. Through neo-and subfunctionalisation, novel functions...
Infection by beet cyst nematodes (BCN, Heterodera schachtii) causes a serious disease of sugar beet, and climatic change is expected to improve the conditions for BCN infection. Yield yield stability under adverse are among main breeding objectives. Breeding tolerant cultivars offering high in presence pathogen therefore relevance.To identify causal genes providing tolerance against infection, we combined several experimental bioinformatic approaches. Relevant genomic regions were detected...
SUMMARY Sugar beet and its wild relatives share a base chromosome number of nine similar morphologies. Yet, interspecific breeding is impeded by sequence divergence that still not fully understood. Since repetitive DNAs are among the fastest evolving parts genome, we investigated, if repeatome innovations losses linked to chromosomal differentiation speciation. We traced genome chromosome‐wide evolution across 13 species comprising all sections genera Beta Patellifolia . For this, combined...
Identification of plasmids from sequencing data is an important and challenging problem related to antimicrobial resistance spread other One-Health issues. We provide a new architecture for identifying plasmid contigs in fragmented genome assemblies built short-read data. employ graph neural networks (GNNs) the assembly propagate information nearby nodes, which leads more accurate classification, especially short that are difficult classify based on sequence features or database searches...
For the identification of a stem cell population, comparison transcriptome data enables simultaneous analysis tens thousands molecular markers and thus precise distinction even closely related populations. Here, we utilized global gene expression profiling to compare two adult human populations, namely neural crest-derived inferior turbinate cells (ITSCs) nasal cavity cardiac (hCSCs) from heart auricle. We detected high similarities between transcriptomes both particularly including range...
Cardiovascular diseases are the number one cause of morbidity and mortality worldwide, but underlying molecular mechanisms remain not well understood. Cardiomyopathies primary heart muscle contribute to high rates failure sudden cardiac deaths. Here, we distinguished four different genetic cardiomyopathies based on gene expression signatures. In this study, RNA-Sequencing was used identify signatures in myocardial tissue cardiomyopathy patients comparison non-failing human hearts. Therefore,...
Abstract Background Infection by beet cyst nematodes (BCN, Heterodera schachtii ) causes a serious disease of sugar beet, and climatic change is expected to improve the conditions for BCN infection. Yield yield stability under adverse are among main breeding objectives. Breeding tolerant cultivars offering high in presence pathogen therefore relevance. Results To identify causal genes providing tolerance against infection, we combined several experimental bioinformatic approaches. Relevant...
ABSTRACT Background Sugar beet ( Beta vulgaris subsp. ) and its crop wild relatives share a base chromosome number of nine similar morphologies. Yet, interspecific breeding is impeded by sequence divergence that still not fully understood. Since repetitive DNA sequences represent the fastest evolving parts genome, they likely impact genomic variability contribute to separation gene pools. Hence, we investigated if innovations losses in repeatome can be linked chromosomal differentiation...
Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to pangenome level. Most analyses classify core genes, which are present all investigated individual genomes, and dispensable only occur single few genomes. The binary classification ‘core’ ‘dispensable’ often based on arbitrary cutoffs presence/absence the analysed Even when extended ‘conditionally dispensable’, this...
Abstract Identification of plasmids from sequencing data is an important and challenging problem related to antimicrobial resistance spread other One-Health issues. In our work, we provide a new architecture for identifying plasmid contigs in fragmented genome assemblies built short-read data. Unlike previous machine-learning approaches this problem, which classify individual separately, employ graph neural networks (GNNs) include information the assembly graph. Propagation nearby nodes...
Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to pangenome level. Most analyses classify core genes, which are present all investigated individual genomes, and dispensable only occur single few genomes. The binary classification ‘core’ ‘dispensable’ often based on arbitrary cutoffs presence/absence the analysed Even when extended ‘conditionally dispensable’, this...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Erich und Hanna Klessmann Foundation Introduction Mutations in human RBM20, encoding the RNA binding protein 20, cause dilated cardiomyopathy (DCM). Within nucleus RBM20 partially colocalies with other splicing factors. Sequencing cardiac transcriptome a RBM20-deficient rat model revealed RBM20-dependent regulation myocardial alternative splicing. We identified several carriers mutations. In literature, it...
Abstract Background As the major source of sugar in moderate climates, sugar-producing beets ( Beta vulgaris subsp. ) have a high economic value. However, low genetic diversity within cultivated requires introduction new traits, for example to increase their tolerance and resistance attributes – traits that often reside crop wild relatives. For this, information beet relatives phylogenetic placements each other are crucial. To answer this need, we sequenced assembled complete plastome...