A5063027686- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- Nitric Oxide and Endothelin Effects
- Liver Disease Diagnosis and Treatment
- Asthma and respiratory diseases
- Chronic Kidney Disease and Diabetes
- Bacteriophages and microbial interactions
- IL-33, ST2, and ILC Pathways
- Sarcoidosis and Beryllium Toxicity Research
- RNA regulation and disease
- Pancreatitis Pathology and Treatment
Indiana University Bloomington
2019-2023
Vanderbilt University
2018-2019
University of Alabama at Birmingham
2016-2018
Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic immunologic investigations suggest common intersections between idiopathic pulmonary (IPF), sarcoidosis, murine models of fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, flow cytometric analysis human specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF...
A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for were inserted into genome segment 7 without compromising expression protein NSP3. These are valuable analyzing rotavirus biology by fluorescence-based live-cell imaging.
Mammalian orthoreovirus (reovirus) is a double-stranded RNA (dsRNA) virus which encapsidates its 10 genome segments within double-layered viral particle. Reovirus infection triggers an antiviral response in host cells serves to limit replication. This initiated by recognition of the incoming sensors present cytoplasm. However, how gain access reovirus unclear, as this dsRNA protected particle proteins throughout infection. To initiate infection, particles are endocytosed and outer layer...
Successful initiation of infection by many different viruses requires their uptake into the endosomal compartment. While some exit this compartment early, others must reach degradative, acidic environment late endosome. Mammalian orthoreovirus (reovirus) is one such penetrating virus. To identify host factors that are important for reovirus infection, we performed a CRISPR-Cas9 knockout (KO) screen targets over 20,000 genes in fibroblasts derived from embryos C57/BL6 mice. We identified...
Abstract Aim Chronic high salt intake exaggerates renal injury and inflammation, especially with the loss of functional ET B receptors. Tauroursodeoxycholic acid (TUDCA) is a chemical chaperone bile that approved for treatment hepatic diseases. Our aim was to determine whether TUDCA reno‐protective in model receptor deficiency chronic salt‐induced inflammation. Methods ‐deficient transgenic control rats were placed on normal (0.8% NaCl) or (8% diet 3 weeks, receiving (400 mg/kg/d; ip)...
ABSTRACT Mammalian orthoreovirus (reovirus) is a double-stranded (ds)RNA virus which encapsidates its 10 genome segments within double-layered viral particle. Reovirus infection triggers an antiviral response in host cells serves to limit replication. This initiated by recognition of the incoming sensors present cytoplasm. However, how gain access reovirus unclear as this dsRNA protected particle proteins throughout infection. To initiate infection, particles are endocytosed and outer layer...
ABSTRACT Successful initiation of infection by many different viruses requires their uptake into the endosomal compartment. While some exit this compartment early, others must reach degradative, acidic environment late endosome. Mammalian orthoreovirus (reovirus) is one such penetrating virus. To identify host factors that are important for reovirus infection, we performed a CRISPR-Cas9 knockout (KO) screen targets over 20,000 genes in fibroblasts derived from embryos C57/BL6 mice. We...
The molecular mechanisms by which the vasoactive peptide endothelin-1 (ET-1) leads to high salt-induced renal damage remain uncertain. These studies were designed determine if ET system induces increasing cellular stress. B deficient (ET def) and transgenic (TG) control rats placed on normal (NSD, 1% NaCl) or salt (HSD, 8% diet for 3 weeks, received daily i.p. injections of stress reliever tauroursodeoxycholic acid (TUDCA, 400 mg/kg/day) vehicle. Blood pressure was monitored telemetry. At...
ABSTRACT Since viruses have limited coding capacity in their genomes, they use host cell machinery to complete virtually every stage of replication cycle. Mammalian orthoreovirus (reovirus) is comprised two concentric protein shells, the inner core and outer capsid. Following attachment its receptor, reovirus enters by receptor-mediated endocytosis. Within endosomes utilizes acid-dependent proteases process viral Specifically, capsid σ3 degraded μ1 cleaved form disassembly intermediate...
ABSTRACT Since viruses have limited coding capacity in their genomes, they use host cell machinery to complete virtually every stage of replication cycle. Mammalian orthoreovirus (reovirus) is comprised two concentric protein shells, the inner core and outer capsid. Following attachment its receptor, reovirus enters by receptor-mediated endocytosis. Within endosomes, utilizes acid-dependent proteases process viral Specifically, capsid σ3 degraded, μ1 cleaved form disassembly intermediate...