A5035624344- Sarcoidosis and Beryllium Toxicity Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Rheumatoid Arthritis Research and Therapies
- Mycobacterium research and diagnosis
- Tracheal and airway disorders
- Research on Leishmaniasis Studies
- Gut microbiota and health
- Drug-Induced Adverse Reactions
- Bacillus and Francisella bacterial research
- IL-33, ST2, and ILC Pathways
- SARS-CoV-2 and COVID-19 Research
- Global Health Workforce Issues
- Diversity and Career in Medicine
- Autoimmune and Inflammatory Disorders
- Pediatric health and respiratory diseases
- Pleural and Pulmonary Diseases
- Nosocomial Infections in ICU
- Cell Adhesion Molecules Research
- Pneumonia and Respiratory Infections
- Respiratory Support and Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
Vanderbilt University Medical Center
2015-2025
Vanderbilt University
2015-2024
University of Maryland, Baltimore
2023-2024
University of Exeter
2024
American Thoracic Society
2020
The Ohio State University
2020
Office of Infectious Diseases
2007-2016
Saint Thomas Health
2005
Saint Thomas West Hospital
2005
Section:ChooseTop of pageAbstract <<ContentsSummary Recommendation...IntroductionMethodsDiagnosisDiagnostic TestingConclusionsReferencesCITING ARTICLES
Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic immunologic investigations suggest common intersections between idiopathic pulmonary (IPF), sarcoidosis, murine models of fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, flow cytometric analysis human specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF...
Effective therapeutic interventions for chronic, idiopathic lung diseases remain elusive. Normalized T-cell function is an important contributor to spontaneous resolution of pulmonary sarcoidosis. Up-regulation inhibitor receptors, such as programmed death-1 (PD-1) and its ligand, PD-L1, are inhibitors function.To determine the effects PD-1 pathway blockade on sarcoidosis CD4(+) proliferative capacity.Gene expression profiles healthy control peripheral blood mononuclear cells were analyzed...
ABSTRACT Sarcoidosis is an enigmatic disease with a pathology similar to that of tuberculosis. We detected Th-1 immune responses Mycobacterium tuberculosis ESAT-6 and KatG peptides from peripheral blood mononuclear cells 15/26 sarcoidosis, 1/24 purified-protein-derivative-negative (PPD−) ( P < 0.0001, Fisher's exact test), 7/8 PPD-positive (PPD+) subjects = 0.21). This finding provides immunologic links between mycobacteria systemic sarcoidosis.
We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis control consisting of mediastinal or cervical lymph nodes lung biopsies. rRNA sequences were amplified 12 (48%) rpoB 6 (24%) the specimens. In total, 15 (60%) but not detected in any tissues (p=0.00002, chi square). three specimens, resembled other than M. tuberculosis. All consistent tuberculosis negative IS6110. provide...
ABSTRACT Considerable evidence supports the concept that CD4 + T cells are important in sarcoidosis pathogenesis, but antigens responsible for observed Th1 immunophenotype remain elusive. The epidemiologic association with bioaerosols and presence of granulomatous inflammation support consideration mycobacterial antigens. To explore role immunopathogenesis, we assessed immune recognition antigens, 6-kDa early secreted antigenic protein (ESAT-6) catalase-peroxidase (KatG), by derived from...
Sarcoidosis is a multisystem granulomatous disease for which the association with mycobacteria continues to strengthen. It hypothesized that single, poorly degradable antigen responsible sarcoidosis pathogenesis. Several reports from independent groups support mycobacterial antigens having role in To identify other microbial targets of adaptive immune response, we tested ability CD4+ and CD8+ T cells recognize multiple antigens.Fifty-four subjects were enrolled this study: 31 patients, nine...
Abstract Sarcoidosis pathogenesis is characterized by peripheral anergy and an exaggerated, pulmonary CD4+ Th1 response. In this study, we demonstrate that anergic responses to polyclonal TCR stimulation are present peripherally within the lungs of sarcoid patients. Consistent with prior observations, spontaneous release IL-2 was noted in sarcoidosis bronchoalveolar lavage T cells. However, contrast hyperactive reported previously, cells displayed stimulation. The correlated diminished...
Sarcoidosis is a chronic granulomatous disease for which there are limited therapeutic options. This the first randomized, placebo-controlled study to demonstrate that antimycobacterial therapy reduces lesion diameter and severity among patients with cutaneous sarcoidosis.To evaluate safety efficacy of once-daily on resolution sarcoidosis lesions.A placebo-controlled, single-masked trial 30 symptomatic lesions deemed require intervention.A tertiary referral dermatology center in Nashville,...
Section:ChooseTop of pageAbstract <<Overview SarcoidosisOverview the Lung Micr...Rationale for GRADS S...Clinical Outcome MeasuresStudy Procedures and Samp...Study AnalysesStudy PotentialConclusionsReferencesCITING ARTICLES
Objectives/Hypothesis Idiopathic subglottic stenosis (iSGS) is a rare and devastating extrathoracic obstruction involving the lower laryngeal upper tracheal airway. It arises without known antecedent injury or associated disease process. Persistent mucosal inflammation localized fibrotic response are hallmarks of disease. Despite initial clinical description iSGS more than 40 year ago, there have been no substantive investigations into pathogenesis this enigmatic progressive airway...
Background Sarcoidosis is a multisystem granulomatous disease of unknown origin with variable and often unpredictable course pattern organ involvement. In this study we sought to identify specific bronchoalveolar lavage (BAL) cell gene expression patterns indicative distinct phenotypic traits. Methods RNA sequencing by Ion Torrent Proton was performed on BAL cells obtained from 215 well-characterised patients pulmonary sarcoidosis enrolled in the multicentre Genomic Research Alpha-1...
Objectives/Hypothesis Idiopathic subglottic stenosis (iSGS) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. Persistent mucosal inflammation a hallmark of disease. Epithelial microbiota dysbiosis found in other chronic inflammatory diseases; however, relationship between composition iSGS unknown. Given critical role for host defense at barriers, we analyzed tissue specimens from patients presence microbial pathogens. Methods Utilizing 30 human iSGS, 20...
HLA-DRB1 is a sarcoidosis risk gene, and the *03:01 allele strongly associated with disease resolution in European cases. Whereas variation susceptibility African Americans, DRB1 alleles are not as well defined, associations have been studied. Associations between genotyped imputed susceptibility/resolution were evaluated sample of 1,277 African-American patients 1,467 control subjects. In silico binding assays performed to assess functional significance alleles. Increased was *12:01 (odds...
Patients with progressive sarcoidosis exhibit increased expression of programmed death-1 (PD-1) receptor on their CD4+ T cells. Up-regulation this marker cell exhaustion is associated a reduction in the proliferative response to (TCR) stimulation, defect that reversed by PD-1 pathway blockade. Genome-wide association studies and microarray analyses have correlated signaling downstream from TCR disease severity, but mechanism not yet known. Reduced phosphatidylinositol 3-kinase (PI3K)/AKT...
As the nation seeks to recruit and retain physician-scientists, gaps remain in understanding addressing mitigatable challenges success of faculty from underrepresented minority (URM) backgrounds. The Doris Duke Charitable Foundation Fund Retain Clinical Scientists program, implemented 2015 at 10 academic medical centers United States, physician-scientists risk leaving science because periods extraordinary family caregiving needs, hardships that URM faculty—especially those who identify as...
Summary Studies of sarcoidosis immunology have noted oligoclonal T cell populations, suggesting cell-mediated immunity that is antigen-specific. Sarcoidosis and pathology are most similar to mycobacterial infections. Mycobacterium tuberculosis infection in mice humans reflects helper 1 (Th1) immune responses multiple wall secreted antigens. We investigated if the response individual subjects could be elicited by antigens performing ex vivo enzyme-linked immunospot assay (ELISPOT) on...