A5005379578- Lipid metabolism and disorders
- Renal Diseases and Glomerulopathies
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Cancer Cells and Metastasis
- Nuclear Receptors and Signaling
- Frailty in Older Adults
- Cancer, Lipids, and Metabolism
- Telomeres, Telomerase, and Senescence
- Hedgehog Signaling Pathway Studies
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Nutrition and Health in Aging
- Metabolism, Diabetes, and Cancer
University of Florida
2022-2025
University of Florida Health
2025
UF Health Cancer Center
2025
Florida College
2023-2024
Cancer cachexia is the involuntary loss of body and skeletal muscle mass, which negatively impacts physical function, quality life, treatment tolerance, survival. Skeletal muscles cachectic people mice with pancreatic tumors also exhibit damage, non-resolute immune cell infiltration, impaired regeneration. These phenotypes may be influenced by accumulation senescent cells, secrete factors detrimental to health. However, there currently no comprehensive research on in tumor-bearing hosts, or...
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An effective cancer therapy requires killing cells and targeting the tumor microenvironment (TME). Searching for molecules critical multiple cell types in TME, we identified NR4A1 as one such molecule that can maintain immune suppressive TME. Here, establish a valid target immunotherapy describe first-of-its-kind proteolysis-targeting chimera (PROTAC, named NR-V04) against NR4A1. NR-V04 degrades within hours vitro exhibits long-lasting degradation tumors with an excellent safety profile....
Renal cell carcinoma (RCC), the most common form of kidney cancer, is a heterogeneous disease with clear RCC (ccRCC) being prevalent and aggressive subtype. While ccRCC tumors have elevated expression angiopoietin-like4 (ANGPTL4), in our study we identified significant subset patients whose cancers show no increase ANGPTL4 expression. These worse prognosis compared to high ANGPTL4. ANGPTL4-low are characterized by increased frequency wild-type Von Hippel-Lindau(WT VHL), gene that commonly...
Abstract B-cell lymphoma X-large (BCL-XL) emerges as a significant player in cancer progression. In our recent findings, we unveil the efficacy of proteolysis-targeting chimeras (PROTACs) directed against BCL-XL, demonstrating their capacity to not only eliminate cells but also target tumor-induced regulatory T (TI-Tregs). Notably, BCL-XL's role promoting breast metastasis extends beyond its anti-apoptotic function. We posit that BCL-XL-targeting PROTACs (BCL-XL-Ps) hold promise for treating...
Abstract Interleukin 2 (IL-2) is the first identified cytokine and its interaction with receptors has been known to shape immune responses in many lymphoid or non-lymphoid tissues for more than four decades. Active T cells are primary cellular source IL-2 production epithelial have never considered major of under physiological conditions. It is, however, tempting speculate that could potentially express regulates intricate interactions between lymphocytes. Datamining our recently published...
<div>Abstract<p>Renal cell carcinoma (RCC), the most common form of kidney cancer, is a heterogeneous disease with clear RCC (ccRCC) being prevalent and aggressive subtype. While ccRCC tumors have elevated expression <i>angiopoietin-</i><i>like</i><i></i>4 (<i>ANGPTL4</i>), in our study we identified significant subset patients whose cancers show no increase <i>ANGPTL4</i> expression. These worse prognosis compared to...
<p>Supplementary Figure S5</p>
<p>Loss of ANGPTL4 is associated with enrichment for lipid metabolism genes. <b>A,</b> Volcano plot CAKi1 A4KO versus WT cells. Genes an absolute value log<sub>2</sub> fold change ≥1 and adjusted <i>P</i> ≤0.05 are indicated. upregulated in A4 KO cells red genes downregulated blue. <b>B,</b> GSEA was performed CAKi-1 Graph depicts the nominal score (NES) gene sets related to that were enriched—had a FDR <0.25—with The indicated by heat...
<p>nANGPTL4 suppresses ccRCC colony formation. <b>A,</b> MTS assay of the indicated CAKi-1 cells. Graph depicts average absorbance ± SD. Two-way ANOVA was done to determine significance (<i>n</i> = 6 all groups). <b>B,</b> The 786O cells were incubated with CSFE and analyzed by flow cytometry. graph % proliferation Welch’s <i>t</i> test significance. <b>C,</b> Soft agar assay. cultured on soft for 30 days number colonies per...
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<p>nANGPTL4 suppresses ccRCC colony formation. <b>A,</b> MTS assay of the indicated CAKi-1 cells. Graph depicts average absorbance ± SD. Two-way ANOVA was done to determine significance (<i>n</i> = 6 all groups). <b>B,</b> The 786O cells were incubated with CSFE and analyzed by flow cytometry. graph % proliferation Welch’s <i>t</i> test significance. <b>C,</b> Soft agar assay. cultured on soft for 30 days number colonies per...
<p>ANGPTL4 suppresses tumor growth of RCC cells with wild-type VHL. <b>A,</b> Graph depicts expression <i>ANGPTL4</i> in CAKi-1 WT or A4KO relative to <i>PPIA</i> as a fold change compared WT. <b>B,</b> 2 × 10<sup>6</sup> the indicated were implanted into flank NOD-SCID/IL2RG (NSG) mice. average volume ± SEM. Two-way ANOVA was done determine significance (<i>n</i> = 8 for both groups). <b>C,</b> Renca...
<p>Supplementary Figure S1</p>
<p>nANGPTL4 inhibits LAL to suppress ccRCC colony formation. <b>A–C,</b> Total intracellular lipids in the indicated CAKi-1 or 786O cells were stained with BODIPY 493/503 and median fluorescence intensity (MFI) was determined. <b>A,</b> Representative histogram is presented for staining of CAKi WT (gray) A4KO (red) cells. <b>B</b> <b>C,</b> Graph depicts average MFI (<b>B</b>) (<b>C</b>) ± SD. Welch’s...
<p>Supplementary Figure S4</p>
<p>nANGPTL4 inhibits LAL to suppress ccRCC colony formation. <b>A–C,</b> Total intracellular lipids in the indicated CAKi-1 or 786O cells were stained with BODIPY 493/503 and median fluorescence intensity (MFI) was determined. <b>A,</b> Representative histogram is presented for staining of CAKi WT (gray) A4KO (red) cells. <b>B</b> <b>C,</b> Graph depicts average MFI (<b>B</b>) (<b>C</b>) ± SD. Welch’s...