A5020351940- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cell death mechanisms and regulation
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Metabolism, Diabetes, and Cancer
- Protein Kinase Regulation and GTPase Signaling
- Alzheimer's disease research and treatments
- Cancer Research and Treatments
- Cholinesterase and Neurodegenerative Diseases
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Occupational and environmental lung diseases
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- Pain Mechanisms and Treatments
- Cardiac electrophysiology and arrhythmias
- Intracranial Aneurysms: Treatment and Complications
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Peptidase Inhibition and Analysis
- Biochemical effects in animals
Research Organization of Information and Systems
2019
Hyogo Medical University
2007-2016
Yamaguchi University
2003
Kobe University
1988-2001
Daiichi University of Pharmacy
2000
Tokyo Institute of Psychiatry
2000
Suita Municipal Hospital
2000
University of California, Irvine
1994-1997
University of Wisconsin–Madison
1992
Yazaki (United States)
1986
Soluble oligomers of amyloid beta (Abeta), rather than fibrils, have been proposed to initiate synaptic and cognitive dysfunction in Alzheimer's disease (AD). However, there is no direct evidence humans that this mechanism can cause AD. Here, we report a novel precursor protein (APP) mutation may provide address question.A Japanese pedigree showing Alzheimer's-type dementia was examined for mutations APP, PSEN1, PSEN2. In addition, 5,310 people, including 2,121 patients with AD, were...
Insulin stimulated translocation of the glucose transporter GLUT4 from cytosol to plasma membrane in a concentration (1 nM-1 μM)-dependent manner and increased uptake 3T3-L1 adipocytes. Insulin-induced cell surface was prevented by phosphoinositide 3 kinase (PI3K) inhibitor wortmannin, 3-phosphoinositide-dependent protein 1 (PDK1) BX912 or Akt1/2 MK2206, knocking-down PI3K, PDK1 Akt1/2. phosphorylation at Thr308/309 Ser473/474, activate Akt1/2, suppressed wortmannin while no significant...
Malignant pleural mesothelioma (MPM) is an aggressive malignant tumor of mesothelial origin that shows a limited response to conventional chemotherapy and radiotherapy. Therefore, diagnosing MPM early very important. Some researchers have previously reported high-mobility group box 1 (HMGB1) was correlated with pulmonary fibrosis. involves the transformation cells, which originate from mesenchymal cells similar lung fibroblasts. Here, we investigated serum levels HMGB1 in patients compared...
Resveratrol, a phytoalexin, reduced the viability of MH7A cells, human rheumatoid arthritis synovial cell line. In apoptosis assay, resveratrol increased TUNEL-positive cells and stimulated H2A.X phosphorylation. Resveratrol disrupted mitochondrial membrane potentials in cytochrome c release from mitochondria to cytosol. activated caspase-3 caspase-9 but not caspase-8 cells. upregulated expression NAD-dependent deacetylase sirtuin 1 mRNA downregulated Bcl-X(L) mRNA, resveratrol-induced...
Our earlier studies suggested crosstalk between IRS/PI3 kinase/PDK1/Akt/Rac1/ROCK and (Shc2/Grb2/SOS)/Ras/Raf/MEK/ERK pathways downstream PDGF-ββ receptor responsible for chemotaxis proliferation of malignant mesothelioma cells. The present study was conducted to obtain evidence this.To assess activation Akt, MEK, ERK, Western blotting carried out on MSTO-211H cells using antibodies against phospho-Thr308-Akt, phopho-Ser473-Akt, phospho-MEK, phopho-ERK1/2, ERK1/2. To knock-down PI3 kinase,...
Extracellular high K(+) (75 mM) increased intracellular Ca(2+) concentrations in cultured rat hippocampal astrocytes, and the rise was abolished by deleting extracellular or cadmium, a non-selective inhibitor of voltage-dependent calcium channels (VDCCs). In reverse transcription-polymerase chain reaction analysis, astrocytes expressed mRNAs for L type-VDCC subunits such as alpha1B, alpha1C, alpha1D, alpha1E. stimulated glutamate release from astrocytes. The not prevented transporter...
The present study examined sphingosine-induced apoptosis in cultured rat hippocampal neurons and astrocytes. Sphingosine induced a concentration (1-100 µM)-dependent manner, that is inhibited by the PKC-δ inhibitor rottlerin, similar effect was obtained with sphingosine kinase inhibitors, to raise intracellular concentrations. increased presence of sphingosine-dependent protein (SDK), suppressed rottlerin. phosphorylated 14-3-3 protein, thereby transforming from dimeric structure into...
Cytoplasmic Ca2+ is a major regulator of exocytosis in secretory cells; however, the Ca(2+)-dependent mechanisms that trigger secretion have not been elucidated. Protein kinase C (PKC) has proposed to be an important component this regulation; effects enzyme on exocytotic apparatus identified. We developed PKC-deficient, semi-intact PC12 cell system which direct stimulatory purified PKC norepinephrine were studied. The reconstitution optimal Ca(2+)-activated by cells required addition MgATP...
Abstract We report 3 surgically treated aneurysms of the distal posterior inferior cerebellar artery (PICA) and review 36 cases in literature. More than half PICA arose to choroidal arch. These lesions often at a turning point (i.e., hairpin curve) an instead junction vessels. Distal are likely be missed it is necessary investigate carefully with four-vessel angiography even if computed tomography done. Eighty-two per cent 39 patients had good recoveries 8% fair results. The mortality rate...
Diffuse malignant peritoneal mesothelioma (DMPM) is an aggressive tumor of mesothelial origin that shows a limited response to cytoreductive surgery along with intraperitoneal chemotherapy. Therefore, early diagnosis DMPM very important. Some researchers have previously reported high-mobility group box 1 (HMGB1) was correlated pulmonary fibrosis. involves the transformation cells, which originate from mesenchymal similar lung fibroblasts. Here, we investigated serum levels HMGB1 in patients...
Brain arteries are structurally characterized by the tight junctions of endothelium and no vasa vasorum that feed themselves. This raises question how brain provided with glucose. A possible explanation is glucose uptake into may be mediated both GLUT1, a facilitative transporter, Na+/glucose cotransporter (SGLT)-like transporter. The functional role for SGLT-like however, unknown. In present study we investigated transporter-operated arterial endothelial cells recording glucose-evoked Na+...
Noradrenaline (NA), released in association with sympathetic nerve sprouting into the dorsal root ganglion (DRG) after peripheral injury, may enhance neuropathic pain. ATP serves as a pain mediator; however, NA-regulated mobilizations DRG is far from understanding. In present study, we analyzed acutely dissociated rat neurons by recording single-channel currents through P2X receptor channels an biosensor outside-out patch-clamp configuration and monitoring real-time enzymatic NADPH...
Background/Aims: Extracellular adenosine induces apoptosis in a variety of cancer cells via diverse signaling pathways. The present study investigated the mechanism underlying adenosine-induced A549 human lung cells. Methods: MTT assay, TUNEL staining, flow cytometry using propidium iodide and annexin V-FITC, real-time RTPCR, Western blotting, monitoring mitochondrial membrane potentials, assay caspase-3, -8, -9 activities were carried out cells, siRNA to silence A3 receptortargeted gene was...
Background/Aims: Growth factors play a critical role in proliferation for variety of cancer cells. The present study was conducted to understand the signaling cascades underlying PDGF-D/PDGF-ββ receptor-mediated mesothelioma Methods: Cell growth and cell cycle were analyzed human non-malignant Met5A cells malignant such as MSTO-211H, NCI-H28, NCI-H2052, NCI-H2452 Results: all used here not affected by PDGF-D, regardless concentrations (1-30 ng/ml) or treatment time (48-72 h). Spontaneous...