A5018706203- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Microbial Inactivation Methods
- RNA and protein synthesis mechanisms
- Plant Pathogenic Bacteria Studies
- Biosensors and Analytical Detection
- Transgenic Plants and Applications
- bioluminescence and chemiluminescence research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Probiotics and Fermented Foods
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Metabolomics and Mass Spectrometry Studies
- Bacterial Identification and Susceptibility Testing
- Pancreatic and Hepatic Oncology Research
- Plant tissue culture and regeneration
- Microbial infections and disease research
- Marine Sponges and Natural Products
- Evolution and Genetic Dynamics
- Essential Oils and Antimicrobial Activity
- Magnetic and Electromagnetic Effects
Jožef Stefan Institute
2020-2024
Jožef Stefan International Postgraduate School
2021-2024
Centre of Excellence for Biosensors, Instrumentation and Process Control
2013-2023
Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality critical for optimal bioprospecting. They used both in early stages of compounds isolation/purification/identification, later evaluate safety efficacy. In this review, we provide a comprehensive overview most common bioassays discovery development new bioactive with focus on marine bioresources. We present list practical considerations selecting appropriate discuss detail typically...
Glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata play a role in biotic stress. In plant genomes, they encoded as relatively large gene families divided into four classes. Most studies virus interactions have focused on glucanases from classes I II. our study, we evaluated β-1,3-glucanase class III (Glu-III) potato–potato YNTN (PVYNTN) interaction implemented findings to biotechnology application. Potato cultivars Désirée Santé, which tolerant...
This study evaluates the multiple-locus variable-number tandem-repeat assay (MLVA) and pulsed-field gel electrophoresis (PFGE) when using restriction enzymes BstZI, SacII, ApaI to fingerprint a diverse collection of methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) sequence type 398 (ST398) isolates. These isolates had been characterized previously by multilocus typing, spa staphylococcal cassette chromosome mec (SCCmec) typing. Typeability discriminatory power were analyzed,...
S. epidermidis is an important opportunistic pathogen causing chronic prosthetic joint infections associated with biofilm growth. Increased tolerance to antibiotic therapy often requires prolonged treatment or revision surgery. Phage currently used as compassionate use and continues be evaluated for its viability adjunctive alternative caused by prevent relapses. In the present study, we report isolation in vitro characterization of three novel lytic phages. Their genome content analysis...
Campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with Campylobacter, a leading cause gastroenteritis worldwide. Eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. Here, this was characterised using gene sequence analysis, neutralisation antiserum host range analysis newly isolated group III Campylobacter phages 68...
Studies of biofilm formation by bacteria are crucial for understanding bacterial resistance and development novel antibacterial strategies. We have developed a new bioluminescence assay Listeria innocua , which is considered non-pathogenic surrogate monocytogenes. L. was transformed with plasmid inducible expression NanoLuc luciferase (Nluc). Concentration-dependent signals were obtained over concentration range more than three log units. This enables absolute quantification cells, the...
Detection of circulating lung cancer cells with cancer-stem like characteristics would represent an improved tool for disease prognosis. However, current antibodies based methods have some disadvantages and therefore cell SELEX (Systematic Evolution Ligands by Exponential Enrichment) was used to develop DNA aptamers, recognizing surface markers non-small carcinoma (NSLC) cells.The human adenocarcinoma line A549 selection in seven cycles. We blood leukocytes negative selection, stem protein...
Growing diversity of protein-based technologies dictates further development bio manufacturing to lower the cost production and maximize yields. Intracellularly expressed recombinant proteins must be extracted from host prior purification. Use electroporation obtain bacteria yeasts has been demonstrated in several studies for different modes operation formats. Here we tested various protocols protein extraction
In Bacteria , a working consensus of species circumscription may have been reached and one the most prominent criteria is high average nucleotide identity (ANI). ANI in effect groups strains that recombine more or less frequently, depending on their biology, as opposed to rare interspecies gene transfer. For bacteriophages, which show various lifestyles, nature fundamental natural unit, if it exists biological sense, not well understood defined. The approaches based dot-plots are useful...
Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used surrogate organism for the foodborne pathogen monocytogenes, because they share genetic and physiological similarities can be in Biosafety Level 1 laboratory. Several methods are evaluate biofilms, including different approaches determine biofilm biomass or culturability, viability, metabolic activity, other microbial community...
ABSTRACT Here, we present the whole-genome sequences of bacteriophages PC5 and PC14 specific for Campylobacter jejuni , a leading cause gastroenteritis in developed countries. Their genomes are syntenic to those group III share more than 90% identity at nucleotide level with members this group.
The development of antimicrobial resistance and the formation Salmonella biofilms are serious public health problems. For this reason, new natural compounds with anti-biofilm activity being sought, wild fungi represent an untapped potential. Various extraction agents, including organic solvents aqueous buffers, can be used to obtain bioactive from sources. To evaluate their bioactivity, extensive screening studies required determine using methods such as broth microdilution or crystal violet...
Abstract Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite control measures applied. As surface of covered with versatile polysaccharides and proteins, these influence interactions any surface. The unique properties high stability fungal proteins make them good candidates for by targeting structures. We screened group lectins protease inhibitors from different species, protein folds known targets their...