A5072879467- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- Alzheimer's disease research and treatments
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Resistance
- Insect-Plant Interactions and Control
- Plant Micronutrient Interactions and Effects
- Proteins in Food Systems
- Cocoa and Sweet Potato Agronomy
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Plant Disease Management Techniques
- Enzyme Structure and Function
- Transgenic Plants and Applications
- Potato Plant Research
- Legume Nitrogen Fixing Symbiosis
- Entomopathogenic Microorganisms in Pest Control
- Antimicrobial Peptides and Activities
- Plant responses to water stress
National Institute of Biology
2016-2025
University of Nova Gorica
2015-2025
Metropolitan University
2020
The purpose of the study was to investigate role salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) potato (Solanum tuberosum L.) Potato virus Y (PVY). responses Ny-1 allele Rywal cultivar and transgenic NahG-Rywal plants that do not accumulate SA were characterized at cytological, biochemical, transcriptome, proteome levels. Analysis noninoculated inoculated leaves revealed HR lesions started develop from 3 d post inoculation completely restricted spread. At...
ABSTRACT Abscission is a tightly regulated process in which plants shed unnecessary, infected, damaged, or aging organs, as well ripe fruits, through predetermined abscission zones response to developmental, hormonal, and environmental signals. Despite its importance, the underlying mechanisms remain incompletely understood. This study highlights deleterious effects of on chloroplast ultrastructure cells tomato flower pedicel zone, revealing spatiotemporal differential gene expression key...
Abstract Abscission occurs specifically in the abscission zone (AZ) tissue as a natural stage of plant development. Previously, we observed delay tomato (Solanum lycopersicum) leaf when LX ribonuclease (LX) was inhibited. The known association between expression and programmed cell death (PCD) suggested involvement PCD abscission. In this study, hallmarks were identified flower AZs during late These included loss viability, altered nuclear morphology, DNA fragmentation, elevated levels...
Potato production is one of the most important agricultural sectors, and it challenged by various detrimental factors, including virus infections. To control losses in potato production, knowledge about virus—plant interactions crucial. Here, we investigated molecular processes plants as a result Y (PVY) infection, economically viral pathogen. We performed an integrative study that links changes metabolome gene expression leaves inoculated with mild PVYN aggressive PVYNTN isolates, for...
Abstract Potato (Solanum tuberosum) is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, flooding, which are severely exacerbated by climate change. Our understanding of crop acclimation stress, however, remains limited. Here, we present a comprehensive molecular physiological high-throughput profiling potato tuberosum, cv. Désirée) under waterlogging applied single or in combinations designed mimic realistic future scenarios. Stress responses were...
Host gene expression changes in the early response to potato virus Y(NTN) interaction were compared two differently sensitive cultivars: resistant cultivar Santé and Igor. Hybridization of TIGR cDNA microarrays allowed us monitor approximately 10,000 genes simultaneously at 0.5 12 h post-inoculation (hpi). Microarray data, analysed by statistics data mining, complemented subtraction library construction sequence analysis validate findings. The profiles cultivars similar faint hpi, but they...
To investigate the dynamics of potato – Potato virus Y (PVY) compatible interaction in relation to salicylic acid - controlled pathways we performed experiments using non-transgenic cv. Désirée, transgenic NahG-Désirée, Igor and PVYNTN, most aggressive strain PVY. The importance viral multiplication symptom development was confirmed by pronounced depleted acid, reversion effect after spraying with 2,6-dichloroisonicotinic (a analogue). We have employed quantitative PCR for monitoring...
SUMMARY Whereas the activation of resistance (R) proteins has been intensively studied, downstream signaling mechanisms leading to restriction pathogen remain mostly unknown. We studied immunity network response conditioned by potato Ny‐1 gene against virus Y. analyzed processes in cell death zone and surrounding tissue on biochemical expression levels order reveal spatiotemporal regulation immune response. show that transcriptional is dependent salicylic acid (SA). For some genes completely...
Differences in the early responses of two potato cultivars, Igor and Nadine, to isolates Potato virus Y (PVY), aggressive PVY NTN mild N , were monitored. Microarray quantitative real‐time PCR analyses carried out identify differentially expressed genes after inoculation with each isolate. Additionally, symptom severity development was observed amount isolate accumulated systemically infected leaves evaluated, where a significantly higher detected. analysis revealed 572, 1288 1706 at 0·5, 12...
Quantitative molecular biology remains a challenge for researchers due to inconsistent approaches control of errors in the final results. Due several factors that can influence result, quantitative analysis and interpretation qPCR data are still not trivial. Together with development high-throughput platforms, there is need tool allowing robust, reliable fast nucleic acid quantification.We have developed "quantGenius" ( http://quantgenius.nib.si ), an open-access web application qPCR-based...
Hypersensitive response (HR)-conferred resistance to viral infection restricts the virus spread and is accompanied by induction of cell death, manifested as formation necrotic lesions. While it known that salicylic acid key component in orchestration events restricting HR, exact function death still unknown. We show potato Y (PVY) can be detected outside zone Ny-1-mediated HR plants (cv. Rywal), observed individual infected cells or small clusters zone. By exploiting features temperature...
The Colorado potato beetle ( Leptinotarsa decemlineata ) is the most important pest of in many areas world. One main reasons for its success lies ability larvae to counteract plant defense compounds. Larvae adapt protease inhibitors (PIs) produced leaves through substitution inhibitor‐sensitive digestive cysteine proteases with inhibitor‐insensitive proteases. To get a broader insight into basis larval adaptation defenses, we created “suppression subtractive hybridisation” library using cDNA...
The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants highly susceptible cultivar Igor was investigated. Virus presence and accumulation analyzed different plant organs tissues using real-time polymerase chain reaction transmission electron microscopy (TEM) negative staining methods. To get a complete insight into location viral RNA within tissue, situ hybridization developed optimized for detection PVY at cellular level. shown to accumulate...
Glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata play a role in biotic stress. In plant genomes, they encoded as relatively large gene families divided into four classes. Most studies virus interactions have focused on glucanases from classes I II. our study, we evaluated β-1,3-glucanase class III (Glu-III) potato–potato YNTN (PVYNTN) interaction implemented findings to biotechnology application. Potato cultivars Désirée Santé, which tolerant...
Abstract Background In viticulture, iron (Fe) chlorosis is a common abiotic stress that impairs plant development and leads to yield quality losses. Under low availability of the metal, applied N form (nitrate ammonium) can play role in promoting or mitigating Fe deficiency stresses. However, processes involved are not clear grapevine. Therefore, aim this study was investigate response two grapevine rootstocks interaction between forms uptake. This process evaluated hydroponic experiment...
Abstract Cystatins, a family of structurally related cysteine proteinase inhibitors, have proved to be useful model system study amyloidogenesis. We extended previous studies the kinetics amyloid‐fibril formation by human stefin B (cystatin B) and some its mutants, proposed an improved for reaction. Overall, observed follow nucleation growth behavior many other amyloidogenic proteins. The minimal kinetic scheme that best fits measurements changes in CD thioflavin T fluorescence as function...
To contribute to the question of putative role cystatins in Alzheimer disease and neuroprotection general, we studied interaction between human stefin B (cystatin B) amyloid-β-(1–40) peptide (Aβ). Using surface plasmon resonance electrospray mass spectrometry were able show a direct two proteins. As an interesting new fact, that binding Aβ is oligomer specific. The dimers tetramers B, which bind Aβ, are domain-swapped as judged from structural studies. Consistent with results, same oligomers...
Abstract Potato is highly water and space efficient but susceptible to abiotic stresses such as heat, drought, or flooding, which are severely exacerbated by climate change. Understanding of crop acclimation stress, however, remains limited. Here, we present a comprehensive molecular physiological high-throughput profiling potato ( Solanum tuberosum , cv. Desirée) under drought waterlogging applied single in combinations designed mimic realistic future scenarios. Stress-responses were...