A5055021255- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Genetically Modified Organisms Research
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Animal Genetics and Reproduction
- Phytoplasmas and Hemiptera pathogens
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Research Data Management Practices
- Chromosomal and Genetic Variations
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
- Scientific Computing and Data Management
- Antimicrobial Peptides and Activities
National Institute of Biology
2015-2024
University of Girona
2006-2012
Structural and functional studies of the coat protein regions potato virus Y reveal crucial roles in viral infectivity.
Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying dsRNA formulations a promising innovative technology providing highly specific and environmentally acceptable option CPB management. We designed silence mesh gene (dsMESH) performed laboratory feeding trials assess impacts on survival development. compared the effectiveness in vivo vitro produced series experiments. additionally...
SUMMARY Whereas the activation of resistance (R) proteins has been intensively studied, downstream signaling mechanisms leading to restriction pathogen remain mostly unknown. We studied immunity network response conditioned by potato Ny‐1 gene against virus Y. analyzed processes in cell death zone and surrounding tissue on biochemical expression levels order reveal spatiotemporal regulation immune response. show that transcriptional is dependent salicylic acid (SA). For some genes completely...
We present a novel multiplex PCR assay for simultaneous detection of multiple transgenic events in maize. Initially, five primers pairs specific to Bt11, GA21, MON810, and NK603, Zea mays L. (alcohol dehydrogenase) were included. The event specificity was based on amplification transgene/plant genome flanking regions, i.e., the same targets as validated real-time assays. These short similarly sized amplicons selected achieve high similar efficiency all targets; however, its unambiguous...
To develop novel crop breeding strategies, it is crucial to understand the mechanisms underlying interaction between plants and their pathogens. Network modeling represents a powerful tool that can unravel properties of complex biological systems. In this study, we aimed use network better immune signaling in potato (Solanum tuberosum). For this, first built on reliable Arabidopsis (Arabidopsis thaliana) model, extending with information from diverse publicly available resources. Next,...
Hypersensitive response (HR)-conferred resistance to viral infection restricts the virus spread and is accompanied by induction of cell death, manifested as formation necrotic lesions. While it known that salicylic acid key component in orchestration events restricting HR, exact function death still unknown. We show potato Y (PVY) can be detected outside zone Ny-1-mediated HR plants (cv. Rywal), observed individual infected cells or small clusters zone. By exploiting features temperature...
Although the reference genome of Solanum tuberosum Group Phureja double-monoploid (DM) clone is available, knowledge on genetic diversity highly heterozygous tetraploid Tuberosum, representing most cultivated varieties, remains largely unexplored. This lack hinders further progress in potato research. In conducted investigation, we first merged and manually curated two existing partially-overlapping DM genome-based gene models, creating a union genes scaffold. Next, compiled available newly...
Summary Besides the intended effects that give a genetically modified (GM) plant desired trait, unintended differences between GM and non‐GM comparable plants may also occur. Profiling technologies allow their identification, number of examples demonstrating are limited diverse have recently been reported. Both from food safety aspect for research purposes, it is important to discern changes produced by transgene its expression those be attributed other factors. Here, we show differential...
MicroRNAs (miRNAs) are small noncoding RNAs, which modulate the abundance and spatiotemporal accumulation of target mRNAs at transcriptional post-transcriptional levels through that play important roles in several biological processes plants. Here we show polyploid species, CRISPR/Cas9 system can be used for fine-tuning miRNA expression, have broader range applications compared to knock-out mutants. We established complete pipeline CRISPR-Cas9-mediated modulation expression potato. It...
Potato virus Y (PVY) is one of the top ten economically most important plant viruses and responsible for major yield losses. We previously suggested involvement N terminal region potato coat protein (CP) in PVY spread. By constructing different deletion mutants, we here show that deletions 40 or more amino acid residues from CP resulted multiplication limited to primary infected cells. Deletion 26 profoundly impaired cell-to-cell movement prevented systemic spread, while 19-23 allowed...
Mitogen-activated protein kinase (MAPK) cascades have crucial roles in the regulation of plant development and responses to stress. Plant recognition pathogen-associated molecular patterns or pathogen-derived effector proteins has been shown trigger activation several MAPKs. This then controls defence responses, including synthesis and/or signalling hormones related genes. The MAPK cascade genes are highly complex interconnected, thus precise mechanisms specific plant–pathogen interactions...
One of the earliest hallmarks plant immune response is production reactive oxygen spe-cies (ROS) in different subcellular compartments, which regulate immunity. A suitable equilibrium, crucial to prevent ROS over-accumulation leading oxidative stress, maintained by salicylic acid (SA), a chief regulator ROS. However, are not only acting downstream SA signaling, but were also proposed be central component self-amplifying loop that regulates signaling as well interaction balance between...
Plant synthetic biology requires software tools to assist on the design of complex multi-genic expression plasmids. Here a vector strategy express genes in plants is formalized and implemented as grammar GenoCAD, Computer-Aided Design for biology. It includes library plant biological parts organized structural categories set rules describing how assemble these into large constructs. Rules developed here are divided three main subsections according aim final construct: protein localization...
Cloning multiple DNA fragments for delivery of several genes interest into the plant genome is one main technological challenges in synthetic biology. Despite modular assembly methods developed recent years, biotechnology community has not widely adopted them yet, probably due to lack appropriate vectors and software tools. Here we present Plant X-tender, an extension highly efficient, scar-free sequence-independent multigene strategy AssemblX, based on overlap-depended cloning rare-cutting...
The pathogenicity of intracellular plant pathogenic bacteria is associated with the action factors/effectors, but their physiological roles for most phytoplasma species, including ‘ Candidiatus Phytoplasma solani’ are unknown. Six putative factors/effectors from six different strains Ca . P. were selected by bioinformatic analysis. way in which they manipulate host cellular machinery was elucidated analyzing Nicotiana benthamiana leaves after Agrobacterium -mediated transient transformation...
Ethylene response factors (ERFs) have been associated with biotic stress in Arabidopsis, while their function non-model plants is still poorly understood. Here we investigated the role of potato ERF StPti5 plant immunity. We show that acts as a susceptibility factor. It negatively regulates immunity against virus Y and Ralstonia solanacearum, pathogens completely different modes action, thereby has than its orthologue tomato. Remarkably, destabilised healthy via autophagy pathway accumulates...
Among the available methods for GMO detection, enforcement and routine laboratories use in practice PCR, based on detection of transgenic DNA. The cost required analysis is constantly increasing due to progress commercialization, with inclusion higher diversity species, traits introduced genetic elements. Thus, analytical technologies must evolve towards high throughput diagnostics. This chapter describes different strategies recently developed tosimultaneously detect/identify many GMOs....