A5033411139- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Algal biology and biofuel production
- Plant Disease Resistance and Genetics
- Plant Micronutrient Interactions and Effects
- Agronomic Practices and Intercropping Systems
- Microbial Metabolic Engineering and Bioproduction
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Microbial Community Ecology and Physiology
- Microbial Metabolites in Food Biotechnology
- Wastewater Treatment and Nitrogen Removal
- Nitrogen and Sulfur Effects on Brassica
- Physiological and biochemical adaptations
University of Vienna
2015-2024
Center for Systems Biology
2013
Digital Proteomics (United States)
2013
Consejo Superior de Investigaciones Científicas
2012
Max Planck Institute of Molecular Plant Physiology
2002-2011
Max Planck Society
2002-2009
University of Potsdam
2008-2009
Leibniz Institute of Vegetable and Ornamental Crops
2009
Charité - Universitätsmedizin Berlin
2009
Universidad Publica de Navarra
2007
Abstract We have established a protocol for the isolation of highly purified peroxisomes from mature Arabidopsis thaliana leaves and analyzed proteome by complementary gel-based gel-free approaches. Seventy-eight nonredundant proteins were identified, which 42 novel had previously not been associated with plant peroxisomes. Seventeen carried predicted peroxisomal targeting signals (PTS) type 1 or 2; 11 contained PTS-related peptides. Peroxisome was supported many in silico analyses confirmed...
A novel method termed metal oxide affinity chromatography (MOAC) of enriching for phosphorylated proteins and peptides based on the phosphate group Al(OH)(3) is presented here. When compared to commercial phosphoprotein-enrichment kits, this more selective, cost effective easily applicable optimization. The use glutamic aspartic acid in loading buffer significantly enhances selectivity. Standard protein mixtures complex Arabidopsis thaliana leaf extracts were tested efficacy enrichment. can...
Abstract Deletion of any the core enzymes photorespiratory cycle, one major pathways plant primary metabolism, results in severe air-sensitivity respective mutants. The peroxisomal enzyme hydroxypyruvate reductase (HPR1) represents only exception to this rule. This indicates presence extraperoxisomal reactions metabolism. We have identified a second reductase, HPR2, and present genetic biochemical evidence that provides cytosolic bypass cycle Arabidopsis thaliana. HPR2 elevated levels other...
Abstract The peribacteroid membrane (PBM) forms the structural and functional interface between legume plant rhizobia. model Lotus japonicus was chosen to study proteins present at PBM by proteome analysis. purified from root nodules an aqueous polymer two-phase system. Extracted were subjected a global trypsin digest. peptides separated nanoscale liquid chromatography analyzed tandem mass spectrometry. Searching nonredundant protein database green expressed sequence tag using spectrometry...
Abstract Drought is one of the environmental factors most affecting crop production. Under drought, symbiotic nitrogen fixation physiological processes to first show stress responses in nodulated legumes. This inhibition process involves a number whose interactions are not yet understood. work aims further understand changes occurring nodules under drought from proteomic perspective. was imposed on Medicago truncatula ‘Jemalong A17’ plants grown symbiosis with Sinorhizobium meliloti strain...
Abstract We present an integrated analysis of the molecular repertoire Chlamydomonas reinhardtii under reference conditions. Bioinformatics annotation methods combined with GCxGC/MS-based metabolomics and LC/MS-based shotgun proteomics profiling technologies have been applied to characterize abundant proteins metabolites, resulting in detection 1069 159 metabolites. Of measured proteins, 204 currently do not EST sequence support; thus a significant portion proteomics-detected provide...
Statistical mining and integration of complex molecular data including metabolites, proteins, transcripts is one the critical goals systems biology (Ideker, T., Galitski, Hood, L. (2001) A new approach to decoding life: biology. Annu. Rev. Genomics Hum. Genet. 2, 343-372). number studies have demonstrated parallel analysis metabolites large scale transcript expression. Protein has been ignored in these studies, although a clear correlation between protein levels shown only rare cases,...
Drought stress hampers plant energy and biomass production; however it is still unknown how internal C:N balance rhizobial symbiosis impact on response to water limitation. Here, the effect of differential optimal nitrogen nutrition root nodule drought rehydration responses Medicago truncatula was assessed. Two groups plants were nodulated with Sinorhizobium medicae or meliloti--differing in performance N fixation; third group grew a rhizobia-free medium received mineral fertilizer. In...
Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved this inhibition are still controversial. Soybean plants were symbiotically grown split-root system (SRS), which allowed for half of root to be irrigated at field capacity while other remained water deprived. NF declined water-deprived nitrogenase activity was maintained control values well-watered half. Concomitantly, amino acids and ureides...
Jellyfish, hydras, corals and sea anemones (phylum Cnidaria) are known for their venomous stinging cells, nematocytes, used prey defence. Here we show, however, that the potent Type I neurotoxin of anemone Nematostella vectensis , Nv1, is confined to ectodermal gland cells rather than nematocytes. We demonstrate massive Nv1 secretion upon encounter with a crustacean prey. Concomitant discharge nematocysts probably pierces prey, expediting toxin penetration. Toxin efficiency in water further...
Nitrogen starvation is known to cause drastic alterations in physiology and metabolism leading the accumulation of lipid bodies many microalgae, it thus presents an important alternative for biofuel production. However, despite importance this process, molecular mechanisms that mediate metabolic remodeling induced by N especially stress recovery are still poorly understood, new candidates bioengineering needed make process useful We have studied changes involved adaptive full vegetative...
Abstract Ammonia oxidation, as the first step of nitrification, constitutes a critical process in global nitrogen cycle. However, fundamental knowledge its key enzyme, copper-dependent ammonia monooxygenase, is lacking, particular for environmentally abundant ammonia-oxidizing archaea (AOA). Here structure enzyme investigated by blue-native gel electrophoresis and proteomics from native membrane complexes two AOA. Besides known AmoABC subunits earlier predicted AmoX, new protein subunits,...
D-GLYCERATE 3-KINASE (GLYK; EC 2.7.1.31) catalyzes the concluding reaction of photorespiratory C2 cycle, an indispensable ancillary metabolic pathway to photosynthetic C3 cycle that enables land plants grow in oxygen-containing atmosphere. Except for GLYK, all other enzymes contribute are known by their primary structures, and encoding genes have been identified. We purified partially sequenced this yet missing enzyme from Arabidopsis thaliana identified it as a putative kinase-annotated...
Regulation of symbiotic nitrogen fixation (SNF) during drought stress is complex and not yet fully understood. In the present work, involvement nodule C N metabolism in regulation SNF Medicago truncatula under a subsequent rewatering treatment was analyzed using combination metabolomic proteomic approaches. Drought induced reduction rates major changes metabolic profile nodules, mostly an accumulation amino acids (Pro, His, Trp) carbohydrates (sucrose, galactinol, raffinose, trehalose). This...
In the era of fast genome sequencing a critical goal is to develop genome-wide quantitative molecular approaches. Here, we present metaproteogenomic strategy integrate proteomics and metabolomics data for systems level analysis in recently sequenced unicellular green algae Chlamydomonas reinhardtii. To achieve representative proteome coverage analysed different growth conditions with protein prefractionation shotgun proteomics. For identification, annotations as well new gene model...
Proteomics has become a critical tool in the functional understanding of plant processes at molecular level. Proteomics-based studies have also contributed to ever-expanding array data modern biology, with many generating Web portals and online resources that contain incrementally expanding updated information. Many these reflect specialist research areas significant novel information is not currently captured by centralized repositories. The Arabidopsis (Arabidopsis thaliana) community well...
Potentilla anserina L. (Rosaceae) is known for its beneficial effects of prevention pre-menstrual syndrome (PMS). For this reason P. processed into many food supplements and pharmaceutical preparations. Here we analyzed hydroalcoholic reference extracts compared them with various different pharmacies using an integrative metabolomics platform comprising GC-MS LC-MS analysis software toolboxes data alignment (MetMAX Beta 1.0) multivariate statistical (COVAIN 1.0). Multivariate statistics the...
The legume Rhizobium symbiosis leads to the formation of a new compartment in plant cell, symbiosome. This harbours bacteroids surrounded by peribacteroid membrane (PBM) originating from plasma membrane. PBM and space between bacteroid membrane, called (PS), mediate exchange metabolites symbionts. Proteome analysis was used as an approach characterise proteins PS. A standard differential centrifugation procedure including Percoll gradient for symbiosome isolation pea root nodules. Proteins...
In the last decade, techniques were established for large scale genome-wide analysis of proteins, RNA, and metabolites, database solutions have been developed to manage generated data sets. The Golm Metabolome Database metabolite (GMD) represents one such effort make these broadly available interconnect different molecular levels a biological system 1. As interpretation in light already existing becomes increasingly important, initiatives are an essential part current future systems...
Summary • Aluminum (Al) toxicity is a major limiting factor of crop production on acid soils, but the implication oxidative stress in this process controversial. A multidisciplinary approach was used here to address question forage legume Lotus corniculatus. Plants were treated with low Al concentrations hydroponic culture, and physiological biochemical parameters, together semiquantitative metabolic proteomic profiles, determined. The exposure plants 10 μM inhibited root leaf growth, had no...
Symbiotic nitrogen fixation is one of the first physiological processes inhibited in legume plants under water-deficit conditions. Despite progress made last decades, molecular mechanisms behind this regulation are not fully understood yet. Recent proteomic work carried out model Medicago truncatula provided indications a possible involvement nodule methionine (Met) biosynthesis and related pathways response to To better understand involvement, drought-induced changes expression content...