A5052544552- Redox biology and oxidative stress
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
- Transgenic Plants and Applications
- Animal Virus Infections Studies
- Mycorrhizal Fungi and Plant Interactions
National Institute of Biology
2021-2024
Summary Hypersensitive response (HR)‐conferred resistance is associated with induction of programmed cell death and pathogen spread restriction in its proximity. The exact role chloroplastic reactive oxygen species link salicylic acid (SA) signaling HR remain unexplained. To unravel this, we performed a detailed spatiotemporal analysis chloroplast redox palisade mesophyll upper epidermis to potato virus Y (PVY) infection resistant genotype transgenic counterpart impaired SA accumulation...
MicroRNAs (miRNAs) are small noncoding RNAs, which modulate the abundance and spatiotemporal accumulation of target mRNAs at transcriptional post-transcriptional levels through that play important roles in several biological processes plants. Here we show polyploid species, CRISPR/Cas9 system can be used for fine-tuning miRNA expression, have broader range applications compared to knock-out mutants. We established complete pipeline CRISPR-Cas9-mediated modulation expression potato. It...
Potato is exceptionally sensitive to a wide range of stresses. Its successful interaction with beneficial symbionts would allow for environmentally friendly plant protection. We investigated the processes involved in colonisation potato Bacillus subtilis. RNAseq showed that at time biofilm establishment, immune responses interacting roots were attenuated, and complex transcriptional network was triggered, including ERFs, MYBs, NACs WRKYs. Interestingly, response intensified if plants are...
Potato virus Y (PVY) is one of the top ten economically most important plant viruses and responsible for major yield losses. We previously suggested involvement N terminal region potato coat protein (CP) in PVY spread. By constructing different deletion mutants, we here show that deletions 40 or more amino acid residues from CP resulted multiplication limited to primary infected cells. Deletion 26 profoundly impaired cell-to-cell movement prevented systemic spread, while 19-23 allowed...
Ethylene response factors (ERFs) have been associated with biotic stress in Arabidopsis, while their function non-model plants is still poorly understood. Here we investigated the role of potato ERF StPti5 plant immunity. We show that acts as a susceptibility factor. It negatively regulates immunity against virus Y and Ralstonia solanacearum, pathogens completely different modes action, thereby has than its orthologue tomato. Remarkably, destabilised healthy via autophagy pathway accumulates...
Abstract Hypersensitive response (HR)-conferred resistance is associated with an induction of programmed cell death (PCD) and pathogen spread restriction in its proximity. While the pivotal role salicylic acid (SA) during HR has been confirmed, exact chloroplastic reactive oxygen species link between SA signalling chloroplast redox state remain unexplained. To unravel these relationships, we performed detailed spatiotemporal analysis to potato virus Y (PVY) infection resistant Ny-1...