A5082921908- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Bioinformatics and Genomic Networks
- Plant Pathogens and Resistance
- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Entomopathogenic Microorganisms in Pest Control
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Biomedical Text Mining and Ontologies
- Plant Parasitism and Resistance
- Gene expression and cancer classification
- Animal Virus Infections Studies
- Fungal and yeast genetics research
- Potato Plant Research
- Plant Physiology and Cultivation Studies
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Plant Molecular Biology Research
- Virus-based gene therapy research
- Plant biochemistry and biosynthesis
- Scientific Computing and Data Management
- Virology and Viral Diseases
National Institute of Biology
2016-2025
University of Zagreb
2019-2024
Cornell University
2023
University of Ljubljana
2023
Genomics and Biotechnology of the Fruits Laboratory
2020
Inštitut Bion
2013
Universidade do Estado do Rio de Janeiro
2012
Universidade Federal do Rio Grande do Sul
2006
Istituto Zooprofilattico Sperimentale delle Venezie
1964-1985
University of Bologna
1967
The Colorado potato beetle is one of the most challenging agricultural pests to manage. It has shown a spectacular ability adapt variety solanaceaeous plants and variable climates during its global invasion, and, notably, rapidly evolve insecticide resistance. To examine evidence rapid evolutionary change, understand genetic basis herbivory resistance, we tested for structural functional genomic changes relative other arthropod species using genome sequencing, transcriptomics, community...
Abstract Arbuscular mycorrhizal (AM) symbiosis can prime plant defenses, leading to mycorrhiza-induced resistance (MIR) against different attackers, including insect herbivores. Still, our knowledge of the complex molecular regulation MIR is very limited. Here, we show that AM fungus Funneliformis mosseae protects tomato plants two chewing herbivores, Spodoptera exigua and Manduca sexta. We explore underlying mechanism through genome-wide transcriptional profiling, bioinformatics network...
The purpose of the study was to investigate role salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) potato (Solanum tuberosum L.) Potato virus Y (PVY). responses Ny-1 allele Rywal cultivar and transgenic NahG-Rywal plants that do not accumulate SA were characterized at cytological, biochemical, transcriptome, proteome levels. Analysis noninoculated inoculated leaves revealed HR lesions started develop from 3 d post inoculation completely restricted spread. At...
PaintOmics is a web server for the integrative analysis and visualisation of multi-omics datasets using biological pathway maps. 4 has several notable updates that improve extend analyses. Three databases are now supported: KEGG, Reactome MapMan, providing more comprehensive knowledge animals plants. New metabolite methods fill gaps in traditional pathway-based enrichment methods. The hub selects compounds with high number significant genes their neighbouring network, suggesting regulation...
During 1981-1983 a disease of pigeons (Columba livia), characterised predominantly by nervous signs, spread across Europe. In the present study 57 viruses isolated from 15 countries (12 European, Japan, Israel and Sudan) were characterised. All shown to be avian paramyxoviruses A/PMV-1 serotype. Monoclonal antibody binding tests showed 53 identical. The virus Sudan was similar these but distinguishable variation. One vaccinal France two virulent Czechoslovakia unrelated other pigeon isolates.
Potato production is one of the most important agricultural sectors, and it challenged by various detrimental factors, including virus infections. To control losses in potato production, knowledge about virus—plant interactions crucial. Here, we investigated molecular processes plants as a result Y (PVY) infection, economically viral pathogen. We performed an integrative study that links changes metabolome gene expression leaves inoculated with mild PVYN aggressive PVYNTN isolates, for...
Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying dsRNA formulations a promising innovative technology providing highly specific and environmentally acceptable option CPB management. We designed silence mesh gene (dsMESH) performed laboratory feeding trials assess impacts on survival development. compared the effectiveness in vivo vitro produced series experiments. additionally...
Stress Knowledge Map (SKM; https://skm.nib.si) is a publicly available resource containing two complementary knowledge graphs that describe the current of biochemical, signaling, and regulatory molecular interactions in plants: highly curated model plant stress signaling (PSS; 543 reactions) large comprehensive network (488 390 interactions). Both were constructed by domain experts through systematic curation diverse literature database resources. SKM provides single entry point for...
Some biological properties of an egg-adapted strain IBDV, PV1, have been studied. By immunofluorescent staining the is serologically identical to another strain, MU2, and three pathogenic strains IBDV. All five do not react with antisera prepared against known avian viruses various groups, including Reoviruses. The PV1 replicates in chick embryo fibro-blasts RK13 cells, formation plaques. highly neuropathogenic for newborn mice, chicks. Various physico-chemical characteristics IBDV studied...
Quantitative molecular biology remains a challenge for researchers due to inconsistent approaches control of errors in the final results. Due several factors that can influence result, quantitative analysis and interpretation qPCR data are still not trivial. Together with development high-throughput platforms, there is need tool allowing robust, reliable fast nucleic acid quantification.We have developed "quantGenius" ( http://quantgenius.nib.si ), an open-access web application qPCR-based...
In the field, plants are challenged by more than one biotic stressor at same time. this study, molecular interactions between potato (Solanum tuberosum L.), Colorado beetle (Leptinotarsa decemlineata Say; CPB) and Potato virus Y(NTN) (PVY(NTN) ) were investigated through analyses of gene expression in leaves gut CPB larvae, release volatile compounds. larval growth was enhanced when reared on secondary PVY(NTN) -infected plants, which linked to decreased accumulation transcripts associated...
Potato virus Y is the most economically important potato viral pathogen. We aimed at unraveling roles of small RNAs (sRNAs) in complex immune signaling network controlling establishment tolerant response cv. Désirée to virus. constructed a sRNA regulatory connecting sRNAs and their targets link level responses physiological processes. discovered an interesting novel sRNAs-gibberellin circuit being activated as early 3 days post inoculation before multiplication can be detected. Two...
Although the reference genome of Solanum tuberosum Group Phureja double-monoploid (DM) clone is available, knowledge on genetic diversity highly heterozygous tetraploid Tuberosum, representing most cultivated varieties, remains largely unexplored. This lack hinders further progress in potato research. In conducted investigation, we first merged and manually curated two existing partially-overlapping DM genome-based gene models, creating a union genes scaffold. Next, compiled available newly...
ABSTRACT Most biosynthetic gene clusters (BGC) encoding the synthesis of important microbial secondary metabolites, such as antibiotics, are either silent or poorly expressed; therefore, to ensure a strong pipeline novel there is need develop rapid and efficient strain development approaches. This study uses comparative genome analysis instruct rational improvement, using Streptomyces rimosus , producer antibiotic oxytetracycline (OTC) model system. Sequencing genomes two industrial strains...
The Colorado potato beetle ( Leptinotarsa decemlineata ) is the most important pest of in many areas world. One main reasons for its success lies ability larvae to counteract plant defense compounds. Larvae adapt protease inhibitors (PIs) produced leaves through substitution inhibitor‐sensitive digestive cysteine proteases with inhibitor‐insensitive proteases. To get a broader insight into basis larval adaptation defenses, we created “suppression subtractive hybridisation” library using cDNA...
Potato is exceptionally sensitive to a wide range of stresses. Its successful interaction with beneficial symbionts would allow for environmentally friendly plant protection. We investigated the processes involved in colonisation potato Bacillus subtilis. RNAseq showed that at time biofilm establishment, immune responses interacting roots were attenuated, and complex transcriptional network was triggered, including ERFs, MYBs, NACs WRKYs. Interestingly, response intensified if plants are...
Staphylococcus aureus is a highly adaptable human pathogen and there constant search for effective antibiotics. Fosfomycin potent irreversible inhibitor of MurA, an enolpyruvyl transferase that uses phosphoenolpyruvate as substrate. The goal this study was to identify the pathways processes primarily affected by fosfomycin at genome-wide transcriptome level aid development new drugs. S. ATCC 29213 cells were treated with sub-MIC concentrations harvested 10, 20 40 minutes after treatment....
Cultivar Desiree is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Desiree, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in high-contiguity chromosome-level assembly. We implemented comprehensive annotation pipeline incorporating gene models annotations from the Solanum tuberosum Phureja DM reference alongside RNA-seq provide high-quality transcript annotations....
Proteins from higher fungi have attracted interest because of their exceptional characteristics. Macrocypins, cysteine protease inhibitors the parasol mushroom Macrolepiota procera , were evaluated for adverse effects and mode action on major potato pest Colorado beetle (CPB, Leptinotarsa decemlineata Say). They shown to reduce larval growth when expressed in or recombinant analogues added diet. Macrocypins target a specific set digestive proteases, intestains. Additionally, protein-protein...