A5100403748- Ion Channels and Receptors
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genetic factors in colorectal cancer
- Plant Pathogens and Fungal Diseases
- Neurobiology and Insect Physiology Research
- Protease and Inhibitor Mechanisms
- Marine Sponges and Natural Products
- Venomous Animal Envenomation and Studies
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Fungal Biology and Applications
- Bioinformatics and Genomic Networks
- Lipid Membrane Structure and Behavior
- Cancer-related gene regulation
- Thyroid Cancer Diagnosis and Treatment
- Machine Learning in Bioinformatics
- Multiple Myeloma Research and Treatments
Institute of Bioinformatics
2019-2025
Manipal Academy of Higher Education
2018-2025
Heidelberg University
2015-2025
German Cancer Research Center
2015-2025
Centre for Nano and Soft Matter Sciences
2024-2025
Medical Advanced Pain Specialists
2025
The Orthopaedic Institute
2025
ICAR Research Complex for NEH Region
2025
CMR University
2025
Kasturba Medical College, Manipal
2024
The Colorado potato beetle is one of the most challenging agricultural pests to manage. It has shown a spectacular ability adapt variety solanaceaeous plants and variable climates during its global invasion, and, notably, rapidly evolve insecticide resistance. To examine evidence rapid evolutionary change, understand genetic basis herbivory resistance, we tested for structural functional genomic changes relative other arthropod species using genome sequencing, transcriptomics, community...
ABSTRACT The detection of samarium (Sm) and its isotopes/ions is gaining significant importance across various fields, including nuclear energy, materials science, environmental monitoring, biomedicine. Accurate sensitive methods are crucial for ensuring safety, quality control, compliance with regulatory standards. Despite the growing need, existing strategies often face challenges such as being time‐consuming, inaccurate, cumbersome, expensive. Surprisingly, fluorometric sensing has been...
Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage filamentous ascomycetes, there is so far only one genome available, namely that black truffle, Tuber melanosporum, mycorrhizal species with unusual subterranean fruiting bodies. To help close sequence gap among basal to allow...
Sturgeons are a group of Condrostean fish with very high evolutionary, economical and conservation interest. The eggs these living fossils represent one the most prized foods animal origin. intense fishing pressure on wild stocks to harvest caviar has caused in last decades dramatic decline their distribution abundance leading International Union for Conservation Nature list them as more endangered species. As direct consequence, world-wide efforts have been made develop sturgeon aquaculture...
Background The Colorado potato beetle (Leptinotarsa decemlineata) is a major pest and serious threat to cultivation throughout the northern hemisphere. Despite its high importance for invasion biology, phenology management, little known about L. decemlineata from genomic perspective. We subjected European adult larval transcriptome samples 454-FLX massively-parallel DNA sequencing characterize basal set of genes this species. created combined assembly datasets including publicly available...
TIICs are critical components of the TME and used to estimate prognostic treatment responses in many malignancies. tumor microenvironment assessed quantified by categorizing immune cells into three subtypes: CD66b+ tumor-associated neutrophils (TANs), FoxP3+ regulatory T (Tregs), CD163+ macrophages (TAMs). In addition, cancers have tumor-infiltrating M1 M2 macrophages, (Neu), CD4+ (T-helper), CD8+ (T-cytotoxic), eosinophils, mast cells. A variety clinical treatments linked cell infiltration...
Transient receptor potential channel subfamily A member 1 (TRPA1) is a non-selective cationic channel, identified initially as cold sensory receptor. TRPA1 responds to diverse exogenous and endogenous stimuli associated with pain inflammation. However, the information on role of toward T-cell responses remains scanty. In silico data suggest that can play an important in activation process. this work, we explored expression its function T cells. By reverse transcription polymerase chain...
Lipoxygenases mediate important biological processes. Through comparative genomics, domain-scan analysis, sequence phylogenetic homology modelling and transcriptional analysis the lipoxygenase gene family of pepper (Capsicum annuum) has been identified. (LOXs) are non-heme, iron-containing dioxygenases playing a pivotal role in diverse processes plants, including defence development. Here, we exploited recent sequencing genome to investigate LOX pepper. Two classes recognized, 9- 13-LOXs...
Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and recombinant enzymes characterized to study their physicochemical properties, ability decolorize textile dyes potential biotechnological applications, activity presence of sea salt. The optimal pH temperature PsLac1 PsLac2 differed relation substrates tested, both were shown be extremely stable at temperatures up 50 °C, retaining 100% after 3 h...
Non-medullary thyroid cancer (NMTC) is a common endocrine malignancy with genetic basis that has yet to be unequivocally established. In recent whole-genome sequencing study of five families occurrence NMTCs, we shortlisted promising variants the help bioinformatics tools. Here, report in silico analyses and vitro experiments on novel germline variant (p.V29L) highly conserved oligonucleotide/oligosaccharide binding domain Protection Telomeres 1 (POT1) gene one families. The results showed...
Familial colorectal cancer (CRC) is only partially explained by known germline predisposing genes. We performed whole-genome sequencing in 15 Polish families of many affected individuals, without mutations CRC focused on loss-of-function variants and functionally characterized them. identified a frameshift variant the CYBA gene (c.246delC) one family splice site TRPM4 (c.25-1 G > T) another family. While both were absent or extremely rare databases, we four additional familial cases two...
Sperm cells exhibit extremely high sensitivity in response to slight changes temperature, osmotic pressure and/or presence of various chemical stimuli. In most cases throughout the evolution, these physico-chemical stimuli trigger Ca2+-signaling and subsequently alter structure, cellular function, motility survival sperm cells. Few reports have recently demonstrated Transient Receptor Potential (TRP) channels from higher eukaryotes, mainly mammals. this work, we explored if lower vertebrates...
Abstract Marine Fungi are potent secondary metabolite producers. However, limited genetic information available their biosynthetic gene clusters (BGCs) and biotechnological applications. To overcome this lack of information, herein, we used next-generation sequencing methods for genome two marine fungi, isolated from the German Wadden Sea, namely Calcarisporium sp. KF525 Pestalotiopsis KF079. The assembled size isolate is about 36.8 Mb with 60 BGCs, while s p. KF079 has a 47.5 harboring 67...
Whole-genome sequencing methods in familial cancer are useful to unravel rare clinically important predisposing variants. Here, we present improvements our pedigree-based variant prioritization pipeline referred as FCVPPv2, including 12 tools for evaluating deleteriousness and 5 intolerance scores missense This is also capable of assessing non-coding regions by combining FANTOM5 data with sets like Bedtools, ChromHMM, Miranda, SNPnexus Targetscan. We tested this a family history papillary...
The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb 16298 putative gene models. identified large non-ribosomal peptide synthetase (nrps1) supporting pks2 in the same biosynthetic cluster. This cluster genes within are functionally active as confirmed by RNA-Seq....