A5074837965- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Fungal Infections and Studies
- Protist diversity and phylogeny
- Antifungal resistance and susceptibility
- RNA and protein synthesis mechanisms
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Resistance
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Light effects on plants
- Enzyme-mediated dye degradation
- Circadian rhythm and melatonin
- Algal biology and biofuel production
- Cellular transport and secretion
- Tannin, Tannase and Anticancer Activities
Ruhr University Bochum
2016-2025
Dartmouth College
2001-2010
Deutsche Nationalbibliothek
2009
Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous have been sequenced, most them by Sanger sequencing. While next-generation sequencing techniques revolutionized genome resequencing, e.g. strain comparisons, genetic mapping, or transcriptome ChIP analyses, de novo assembly eukaryotic still presents significant hurdles, because their large size stretches repetitive sequences....
Tuberaceae is one of the most diverse lineages symbiotic truffle-forming fungi. To understand molecular underpinning ectomycorrhizal truffle lifestyle, we compared genomes Piedmont white (Tuber magnatum), Périgord black melanosporum), Burgundy aestivum), pig (Choiromyces venosus) and desert (Terfezia boudieri) to saprotrophic Pezizomycetes. Reconstructed gene duplication/loss histories along a time-calibrated phylogeny Ascomycetes revealed that Tuberaceae-specific traits may be related...
Mating-type genes in fungi encode regulators of mating and sexual development. Heterothallic ascomycete species require different sets mating-type to control nonself-recognition compatible partners types. Homothallic (self-fertile) also carry their genome that are essential for To analyze the molecular basis homothallism role during fruiting-body development, we deleted each three genes, SmtA-1 (MAT1-1-1), SmtA-2 (MAT1-1-2), SmtA-3 (MAT1-1-3), contained MAT1-1 part locus homothallic Sordaria...
Lichtheimia species are the second most important cause of mucormycosis in Europe. To provide broader insights into molecular basis pathogenicity-associated traits basal Mucorales, we report full genome sequence L. corymbifera and compared it to Rhizopus oryzae, common worldwide. The assembly encompasses 33.6 MB 12,379 protein-coding genes. This study reveals four major differences R. oryzae: (i) presence an highly elevated number gene duplications which unlike oryzae not due whole...
NADPH oxidase (NOX)-derived reactive oxygen species (ROS) act as signaling determinants that induce different cellular processes. To characterize NOX function during fungal development, we utilized the genetically tractable ascomycete Sordaria macrospora. Genome sequencing of a sterile mutant led us to identify encoding nox1 gene required for fruiting body formation, regular hyphal growth, and fusion. These phenotypes are shared by nor1, lacking regulator NOR1. Further phenotypic analyses...
Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage filamentous ascomycetes, there is so far only one genome available, namely that black truffle, Tuber melanosporum, mycorrhizal species with unusual subterranean fruiting bodies. To help close sequence gap among basal to allow...
Abstract The advent of complex multicellularity (CM) was a pivotal event in the evolution animals, plants and fungi. In fungal Ascomycota, CM is based on hyphal filaments arose Pezizomycotina. genus Neolecta defines an enigma: phylogenetically placed related group containing mostly yeasts, nevertheless possesses Pezizomycotina-like CM. Here we sequence irregularis genome identify CM-associated functions by searching for genes conserved Pezizomycotina, which are absent or divergent budding...
Agrobacterium species are capable of interkingdom gene transfer between bacteria and plants. The genome tumefaciens consists a circular linear chromosome, the At-plasmid Ti-plasmid, which harbors bacterial virulence genes required for tumor formation in Little is known about promoter sequences small RNA (sRNA) repertoire this other α-proteobacteria. We used differential sequencing (dRNA-seq) approach to map transcriptional start sites 388 annotated operons. In addition, total number 228...
The study of mutants to elucidate gene functions has a long and successful history; however, discover causative mutations in that were generated by random mutagenesis often takes years laboratory work requires previously genetic and/or physical markers, or resources like DNA libraries for complementation. Here, we present an alternative method identify defective genes developmental the filamentous fungus Sordaria macrospora through Illumina/Solexa whole-genome sequencing. We sequenced pooled...
Abstract Background During sexual development, filamentous ascomycetes form complex, three-dimensional fruiting bodies for the protection and dispersal of spores. Fruiting contain a number cell types not found in vegetative mycelium, these morphological differences are thought to be mediated by changes gene expression. However, little is known about spatial distribution expression fungal development. Here, we used laser microdissection (LM) RNA-seq determine patterns young (protoperithecia)...
Species within the human pathogenic Cryptococcus species complex are major threats to public health, causing approximately 1 million annual infections globally. amylolentus is most closely known related of complex, and it non-pathogenic. Additionally, while have bipolar mating systems with a single large type (MAT) locus that represents derived state in Basidiomycetes, C. has tetrapolar system 2 MAT loci (P/R HD) located on different chromosomes. Thus, studying will shed light transition...
Significance Bacterial secondary metabolites are of great relevance to human society and the environment. To this day, investigations secreted focus on single compounds, compound classes, or compounds with specific bioactivities. Comparing supernatants Streptomyces chartreusis cultivated in different media, using liquid chromatography–coupled tandem MS, we detected a diversity highly regulated surpassing genome-based expectations. Guided by molecular networking, new polyether ionophore was...
ABSTRACT Microbial fermentation of agro-industrial waste holds great potential for reducing the environmental impact associated with production lipids industrial purposes from plant biomass. However, chemical complexity many residues currently prevents efficient conversion into lipids, creating a high demand strains ability to utilize all energy-rich components agricultural residues. Here, we present results genome and transcriptome analyses Trichosporon oleaginosus . This oil-accumulating...
ABSTRACT Fungal sexual reproduction is controlled by the mating-type ( MAT ) locus. In contrast to a majority of species in phylum Basidiomycota that have tetrapolar systems, opportunistic human pathogen Cryptococcus neoformans employs bipolar system, with two mating types and α) determined single locus unusually large (~120 kb) contains more than 20 genes. While several genes are associated development, others control conserved cellular processes (e.g., cargo transport protein synthesis),...
A major goal of evolutionary biology is to identify the genetic basis for emergence complex adaptive traits. Diatoms are ancestrally photosynthetic microalgae. However, in genus Nitzschia , loss photosynthesis led a group free-living secondary heterotrophs whose manner acquiring chemical energy unclear. Here, we sequence genome non-photosynthetic diatom sing1 and its catabolism brown algal cell wall polysaccharide alginate. N . obtained an endolytic alginate lyase enzyme by horizontal gene...
Fruiting body formation in ascomycetes is a highly complex process that under polygenic control and fundamental part of the fungal sexual life cycle. However, molecular determinants regulating this cellular are largely unknown. Here we show sterile pro40 mutant defective 120-kDa WW domain protein plays pivotal role fruiting maturation homothallic ascomycete Sordaria macrospora. Although domains occur many eukaryotic proteins, homologs PRO40 present only filamentous ascomycetes....
RNA editing is a post-transcriptional process that modifies molecules leading to transcript sequences differ from their template DNA. A-to-I was found be widely distributed in nuclear transcripts of metazoa, but detected fungi only recently study the filamentous ascomycete Fusarium graminearum revealed extensive mRNAs sexual structures (fruiting bodies). Here, we searched for putative events RNA-seq data Sordaria macrospora and Pyronema confluens, two distantly related ascomycetes,...
Speciation is a central mechanism of biological diversification. While speciation well studied in plants and animals, comparison, relatively little known about fungi. One fungal model the Cryptococcus genus, which best for pathogenic neoformans/Cryptococcus gattii species complex that causes >200,000 new human infections annually. Elucidation how these evolved into important human-pathogenic remains challenging can be advanced by studying most closely related nonpathogenic species,...