A5085608528- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Gold and Silver Nanoparticles Synthesis and Applications
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
- Zebrafish Biomedical Research Applications
- Influenza Virus Research Studies
- RNA Interference and Gene Delivery
- Spectroscopy and Chemometric Analyses
- Immunotherapy and Immune Responses
- Chemical Synthesis and Analysis
- Wnt/β-catenin signaling in development and cancer
- Cleft Lip and Palate Research
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
Peking University
2012-2025
State Key Laboratory of Natural and Biomimetic Drugs
2021-2025
Scripps Research Institute
2017-2023
Beijing National Laboratory for Molecular Sciences
2011-2020
Center for Life Sciences
2012-2020
King Center
2020
Abstract Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within Raman‐silent region of cell. Coupling this spectroscopic signature with microscopy yields new imaging modality beyond fluorescence label‐free microscopies. The bioorthogonal various tagged an alkyne by state‐of‐the‐art technique, stimulated (SRS) microscopy, is reported....
A cell-specific metabolic glycan labeling strategy has been developed using azidosugars encapsulated in ligand-targeted liposomes. The ligands are designed to bind specific cell-surface receptors that only expressed or up-regulated target cells, which mediates the intracellular delivery of azidosugars. delivered metabolically incorporated into glycans, then imaged via a bioorthogonal reaction.
Direct detection of glycans on live cells using surface-enhanced Raman scattering (SERS) has been shown. A bioorthogonal reporter was directly installed onto the monosaccharide analogs. Once metabolically incorporated into cell surface glycans, detected SERS (see picture).
Proteins are the workhorses of cell, playing crucial roles in virtually every biological process. The revolutionary ability to visualize and monitor proteins living systems, which is largely result development green fluorescence protein (GFP) its derivatives, has dramatically expanded our understanding dynamics function. Still, GFPs ill suited many circumstances; one major drawback their relatively large size, can significantly perturb functions native they fused.To bridge this gap,...
Sialic acid analogues containing a unique chemical functionality or reporter have been metabolically incorporated into sialylated glycans. This process, termed metabolic glycan labeling, has emerged as powerful tool for studying sialylation well other types of glycosylation. Currently, this technique can install only single functionality. Here we describe strategy dual labeling glycans using new class bifunctional sialic two distinct reporters at the N-acyl and C9 positions. These unnatural...
Abstract Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, remains challenging to selectively visualize the cancer glycome vivo. Herein, a strategy for targeted imaging of tumor‐associated glycans by using ligand‐targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles bound cancer‐cell‐specific receptors and installed azides into melanoma xenograft mouse model tissue‐specific...
Abstract CD22, a member of Siglec family sialic acid binding proteins, has restricted expression on B cells. Antibody‐based agents targeting CD22 or CD20 lymphoma and leukemia cells exhibit clinical efficacy for treating these malignancies, but also attack normal leading to immune deficiency. Here, we report chemoenzymatic glycocalyx editing strategy introduce high‐affinity specific ligands onto NK‐92MI cytokine‐induced natural killer achieve tumor‐specific targeting. These CD22‐ligand...
Abstract Chemoenzymatic modification of cell-surface glycan structures has emerged as a complementary approach to metabolic oligosaccharide engineering. Here, we identify Pasteurella multocida α2-3-sialyltransferase M144D mutant, Photobacterium damsela α2-6-sialyltransferase, and Helicobacter mustelae α1-2-fucosyltransferase, efficient tools for live-cell modification. Combining these enzymes with pylori α1-3-fucosyltransferase, develop host-cell-based assay probe glycan-mediated influenza A...
Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development and organ formation. Aberrant regulation of EMT often leads to tumor progression. Changes cell surface sialylation have recently been implicated mediating EMT. Herein we report the visualization dynamic changes glycoproteomic analysis newly synthesized sialylated proteins by metabolic labeling glycans with azides, followed click fluorophores or affinity tags. We discovered that was down-regulated during...
Metabolic labeling of glycans with bioorthogonal reporters has been widely used for glycan imaging and glycoproteomic profiling. One the intrinsic limitations metabolic is lack cell-type selectivity. The recently developed liposome-assisted reporter (LABOR) strategy provides a promising means to overcome this limitation, but mechanism LABOR not investigated in detail. In work, we performed mechanistic study on explored its multiplexing capability. Our studies support an endocytosis-salvage...
Sialic acid-binding immunoglobulin-like lectins, also known as Siglecs, have recently been designated glyco-immune checkpoints. Through their interactions with sialylated glycan ligands overexpressed on tumor cells, inhibitory Siglecs innate and adaptive immune cells modulate signaling cascades to restrain anti-tumor responses. However, the elucidation of mechanisms underlying these processes is just beginning. We find that when human natural killer (NK) attack remodeling occurs target at...
Zuckersüß: Glykane auf lebenden Zellen konnten direkt mithilfe von oberflächenverstärkter Raman-Streuung (SERS) detektiert werden. Monosaccharid-analoge Verbindungen wurden mit einem bioorthogonalen Raman-Reporter modifiziert. Sobald der in den Zelloberflächen-Glykanen metabolisch eingebaut war, konnte er SERS werden (siehe Bild).
Live imaging of biomolecules with high specificity and sensitivity as well minimal perturbation is essential for studying cellular processes. Here, we report the development a bioorthogonal surface-enhanced Raman scattering (SERS) approach that exploits small reporters visualizing cell-surface biomolecules. The cells were cultured imaged by SERS microscopy on arrays Raman-enhancing nanoparticles coated silicon wafers or glass slides. including azides, alkynes, carbondeuterium bonds are in...
Abstract Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within Raman‐silent region of cell. Coupling this spectroscopic signature with microscopy yields new imaging modality beyond fluorescence label‐free microscopies. The bioorthogonal various tagged an alkyne by state‐of‐the‐art technique, stimulated (SRS) microscopy, is reported....
Abstract Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, remains challenging to selectively visualize the cancer glycome vivo. Herein, a strategy for targeted imaging of tumor‐associated glycans by using ligand‐targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles bound cancer‐cell‐specific receptors and installed azides into melanoma xenograft mouse model tissue‐specific...
Abstract Truncated mucin‐type O‐glycans, such as Tn‐associated antigens, are aberrantly expressed biomarkers of cancer, but remain challenging to target. Reactive antibodies these antigens either lack high affinity or prone antigen escape. Here, we have developed a robust chemoenzymatic strategy for the global labeling i. e. Tn (GalNAcα‐O‐Ser/Thr), Thomsen‐Friedenreich (Galβ1‐3GalNAcα‐O‐Ser/Thr, TF) and STF (Neu5Acα2‐3Galβ1‐3GalNAcα‐O‐Ser/Thr, STF) in human whole blood with efficiency...
Host cell surface glycans play critical roles in influenza virus A (IVA) infection ranging from modulation of IVA attachment to membrane fusion and host tropism. Approaches for quick sensitive profile viral avidity toward a specific type glycan can contribute the understanding tropism switching among different strains. Here, we developed method based on chemoenzymatic engineering investigate possible involvement α1-2-fucosides infections. Using truncated human fucosyltransferase 1 (hFUT1),...