A5100413659- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Optical Imaging and Spectroscopy Techniques
- Dermatology and Skin Diseases
- Photoacoustic and Ultrasonic Imaging
- Acupuncture Treatment Research Studies
- Acute Myeloid Leukemia Research
- Non-Invasive Vital Sign Monitoring
- Pain Management and Placebo Effect
- Renal and related cancers
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Alzheimer's disease research and treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Digital Radiography and Breast Imaging
- Phytochemical Studies and Bioactivities
- CAR-T cell therapy research
- Neurobiology and Insect Physiology Research
- Electrowetting and Microfluidic Technologies
- Insect behavior and control techniques
- Olfactory and Sensory Function Studies
- RNA Research and Splicing
- Neutropenia and Cancer Infections
- Flavonoids in Medical Research
- Multiple and Secondary Primary Cancers
Harvard University
2012-2025
Center for Systems Biology
2019-2025
Anhui University of Science and Technology
2025
Xi'an Jiaotong University
2025
Massachusetts General Hospital
2004-2024
Ningbo University
2024
First Affiliated Hospital of Hebei Medical University
2023
Hebei Medical University
2023
Athinoula A. Martinos Center for Biomedical Imaging
2004-2015
Peking University
2012-2014
Significance We report a quantitative Raman microscopy method that measures the concentration of protein and lipid in cells at high spatial resolution living fixed samples tissues, allowing studies cell size organelle regulation both culture tissue slices; it can be applied to problems control, intracellular crowding, metabolism context growth, differentiation, senescence, pathology.
Abstract Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within Raman‐silent region of cell. Coupling this spectroscopic signature with microscopy yields new imaging modality beyond fluorescence label‐free microscopies. The bioorthogonal various tagged an alkyne by state‐of‐the‐art technique, stimulated (SRS) microscopy, is reported....
Tooth is vital not only for a good smile, but also health. Yet, we lose tooth regularly due to accidents or diseases. An ideal solution this problem regenerate with patients' own cells. Here describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells (ifhU-iPSCs).We first differentiated ifhU-iPSCs epithelial sheets, which were then recombined E14.5 mouse dental mesenchymes. Tooth-like recovered these recombinants in 3 weeks success rate...
Itch is an aversive sensory experience and while systemic therapies, such as acupuncture, have shown promise in alleviating itch patients suffering from chronic itch, their antipruritic mechanisms are unknown. As several lines of evidence implicate brain-focused mechanisms, we applied functional magnetic resonance imaging our validated temperature-modulation model to evaluate the underlying brain circuitry supporting allergen-induced reduction atopic dermatitis by antihistamine, respective...
We present an algorithm to reconstruct chromosphere concentration images directly rather than following the traditional two-step process of reconstructing wavelength-dependent absorption coefficient and then calculating images. This procedure imposes prior spectral information into image reconstruction that results in a dramatic improvement contrast-to-noise ratio better 100%. demonstrate this with simulations dynamic blood phantom experiment.
Quantitative single-cell analysis enables the characterization of cellular systems with a level detail that cannot be achieved ensemble measurement. In this Feature we explore quantitative imaging applications nonlinear microscopy techniques. We first offer an introductory tutorial on optical processes and then survey range techniques have proven to useful for live cell without fluorescent labels.
Background Psychological factors are known to significantly modulate itch in patients suffering from chronic itch. Itch is also highly susceptible both placebo and nocebo (negative placebo) effects. Brain activity likely supports nocebo-induced itch, but currently unknown. Methods We collected functional MRI (fMRI) data atopic dermatitis (AD) patients, a within-subject design, contrast brain response saline understood be allergen vs open-label control. Exploratory analyses compared results...
Circuit design for control of metabolism A transcriptional mechanism in yeast that allows cells to respond changes nutrient concentrations works very much like a household light-dimmer switch. That is, the system separately controls whether gene expression is “on” or “off” and extent expression. The galactose-responsive pathway activated when need switch from metabolizing glucose galactose. Ricci-Tam et al. found that, rather than using two separate elements dimmer controls, use single...
We developed a simple method to construct liquid-core/PDMS-cladding optical waveguides through pressurized filling of dead-ended micro-channels with fluids. The are in the same layer as microfluidic channels which greatly simplifies device fabrication. With proper contrast between refractive index core and cladding, transmission loss is less than 5 dB cm−1. also create flat optically clear surfaces on sides PDMS devices order couple light free-space embedded inside chip. these newly...
Chronic itch, a highly debilitating condition, has received relatively little attention in the neuroimaging literature. Recent studies suggest that brain regions supporting itch chronic patients encompass sensorimotor and salience networks, corticostriatal circuits involved motor preparation for scratching. However, how these different areas interact with one another context of is still unknown. We acquired BOLD fMRI scans 14 atopic dermatitis to investigate resting-state functional...
Abstract Tissue interactions play a crucial role in tooth development. Notably, extracellular vesicle-mediated between the mandible and germ are considered essential. Here, we revealed that vesicles could modulate proliferation differentiation of dental mesenchymal cells by regulating histone demethylase KDM2B. Further investigation showed derived deliver miR-206 to KDM2B, thereby An animal study demonstrated miR-206/KDM2B pathway affected morphogenesis mineralization after eight weeks...
UBQLN1 variants have been associated with increased risk for late-onset Alzheimer's disease (AD). We produced transgenic Drosophila models that either silence (by RNAi) or overexpress the ortholog of human UBQLN1, dUbqln. Silencing dUbqln in central nervous system led to age-dependent neurodegeneration and shortened lifespan. wing vein loss could be partially rescued by mutant rhomboid (rho), a known component epidermal growth factor receptor signaling pathway. Conversely, overexpression...
Mutations in the presenilin genes cause majority of early-onset familial Alzheimers disease. Recently, mutations have been identified patients with dilated cardiomyopathy (DCM), a common heart failure and most prevalent diagnosis cardiac transplantation patients. However, molecular mechanisms, by which lead to either AD or DCM, are not yet understood. We employed transgenic Drosophila models optical coherence tomography imaging technology analyze function live adult Drosophila. Silencing...
The SRY-related HMG-box 5 (SOX5) gene encodes a member of the SOX family transcription factors.Recently, genome-wide association studies have implicated SOX5 as candidate for susceptibility to four cardiacrelated endophenotypes: higher resting heart rate (HR), electrocardiographic PR interval, atrial fibrillation and left ventricular mass.We determined that human has highly conserved Drosophila ortholog, Sox102F, employed transgenic models quantitatively measure cardiac function in adult...
Abstract Leukemia is a leading cause of cancer death. Recently, the latexin (Lxn) gene was identified as potential tumor suppressor in several types solid tumors and lymphoma, Lxn expression found to be absent or downregulated leukemic cells. Whether functions leukemia what molecular cellular mechanisms are involved unknown. In this study, myeloid leukemogenic FDC-P1 cell line used model system ectopically expressed these Using protein pull-down assay mass spectrometry, ribosomal subunit 3...
Stimulated Raman scattering (SRS) microscopy is a label-free chemical imaging technique. Two-color often necessary to determine the distribution of species in SRS microscopy. Current multi-color methods involve complicated instrumentation or longer data acquisition time are limited transmission imaging. In this Letter, we show that by adding simple fiber amplifier 2 ps laser source and optical-parametric-oscillator-based setup, one can achieve simultaneous two-color frequency modulation The...
Abstract Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within Raman‐silent region of cell. Coupling this spectroscopic signature with microscopy yields new imaging modality beyond fluorescence label‐free microscopies. The bioorthogonal various tagged an alkyne by state‐of‐the‐art technique, stimulated (SRS) microscopy, is reported....
Abstract Cell mass and its chemical composition are important aggregate cellular variables for physiological processes including growth control tissue homeostasis. Despite their central importance, it has been difficult to quantitatively measure these quantities from single cells in intact tissue. Here, we introduce Normalized Raman Imaging (NoRI), a Stimulated Scattering (SRS) microscopy method that provides the local concentrations of protein, lipid water live or fixed samples with high...
Purpose Chronic itch is a prevalent symptom of many inflammatory skin disorders, including atopic dermatitis (AD). While conventional systemic approaches to reduce AD have shown limited efficacy and/or significant side effects, several recent studies demonstrated effectiveness acupuncture for reducing in healthy adults and AD. We sought evaluate the brain circuitry underlying reduction itch.
Background: Hilar cholangiocarcinoma (HCCA) has a high degree of malignancy and poor prognosis, the best long-term prognosis can only be achieved by radical resection. However, surgical steps are complicated, operating space is limited, making it hard to complete laparoscopically. So our team proposes new approach for laparoscopic left-liver-first anterior modular orthotopic right hemihepatectomy (Lap-Larmorh). In this way, we simplify operation reduce difficulty. Materials Methods: We...