A5002228948- Digital Holography and Microscopy
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Advancements in Battery Materials
- Optical measurement and interference techniques
- Mechanical and Optical Resonators
- Advanced Battery Technologies Research
- Erythrocyte Function and Pathophysiology
- Advanced X-ray Imaging Techniques
- Bacterial Genetics and Biotechnology
- Near-Field Optical Microscopy
- Advanced Battery Materials and Technologies
- Multiple Myeloma Research and Treatments
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Sensors
- Gene Regulatory Network Analysis
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
Hanyang University
2023-2024
Center for Systems Biology
2015-2024
Harvard University
2013-2024
Anyang University
2024
University of California, San Diego
2024
University of Wisconsin–Milwaukee
2024
Harvard University Press
2024
Hanwha Solutions (South Korea)
2019
University of Illinois Urbana-Champaign
2014
Eulji University
2013
Significance We report a quantitative Raman microscopy method that measures the concentration of protein and lipid in cells at high spatial resolution living fixed samples tissues, allowing studies cell size organelle regulation both culture tissue slices; it can be applied to problems control, intracellular crowding, metabolism context growth, differentiation, senescence, pathology.
Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density single cells in suspension 1% 0.03%, respectively. We find that transformed murine lymphocytic leukemia mouse pro–B cell lymphoid lines, mitotic reversibly increase their by more than 10% decrease 0.4% over 20-min period....
Significance Accurate measurement of cell size is critical in studies growth. Optical methods based on interferometry are known to be suitable for attached cells, but the existing techniques were originally designed thin samples and not ideal thick ones, such as mitotic cells. Synthetic phase microscopy (SPM), a new tomographic interferometric method, offers an elegant solution this problem. This paper demonstrates ability SPM measure growth mammalian cells accurately, it clear requirement...
Cell dry mass is principally determined by the sum of biosynthesis and degradation. Measurable change in occurs on a time scale hours. By contrast, cell volume can minutes altering osmotic conditions. How changes are coupled fundamental question size control. If were proportional to during growth, would always maintain same cellular density, defined as dividing volume. The accuracy stability against perturbation this proportionality has never been stringently tested. Normalized Raman Imaging...
In visualizing transparent biological cells and tissues, the phase contrast microscope its related techniques have been a cornerstone of nearly every cell biology laboratory. However, methods are inherently qualitative lack in 3-D imaging capability. We introduce novel tomographic microscopy for quantitative three-dimensional mapping refractive index live tissues using phase-shifting laser interferometric with variable illumination angle.
Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations the nonquantitative nature imaging. To overcome this problem, we developed quantitative DIC based on off-axis sample self-interference. The digital holography algorithm applied to obtain phase gradients in orthogonal directions, which leads image through spiral integration gradients. This practically simple...
The fine balance of growth and division is a fundamental property the physiology cells, one least understood. Its study has been thwarted by difficulties in accurate measurement cell size even greater challenges measuring single over time. We address these limitations demonstrating computationally enhanced methodology for quantitative phase microscopy adherent using improved image processing algorithms automated cell-tracking software. Accuracy more than twofold this improvement sufficient...
We describe a heterodyne Mach-Zehnder interferometric microscope capable of quantitative phase imaging biological samples with subnanometer sensitivity and frame rates up to 10 kHz. use the image cultured neurons measure nanometer-scale voltage-dependent motions in cells expressing membrane motor protein prestin.
We report a quantitative phase microscope based on spectral domain optical coherence tomography and line-field illumination. The line illumination allows self phase-referencing method to reject common-mode noise. also features separate reference arm, permitting the use of high numerical aperture (NA > 1) objectives for resolution measurement at multiple points along demonstrate that path-length sensitivity instrument can be as good 41 pm/square root Hz, which makes it suitable nanometer...
Improving lithium-ion transport in electrodes by controlling electrode microstructure is a promising option for enhancing the fast-charging capability of graphite anodes batteries. Dry processing based on polytetrafluoroethylene binder has attracted considerable attention as an alternative to solvent-based wet processing. The morphology particles significant impact microstructure, but few reports have been published dry-processed anodes. In this work, we found that key factor determine well...
Using a first-principles method, we study field emission properties of double-wall carbon nanotubes with various end geometries and combinations inner outer walls. The open exhibit substantial contribution to the current from extended π states. These states are insensitive detailed tip structure or presence foreign adsorbed molecules resulting is expected be stable under moderate vacuum environment. mechanical stiffness also increases significantly compared single-wall nanotube, ensuring...
Accurate refractive index measurement in the deep ultraviolet (UV) range is important for separate quantification of biomolecules such as proteins and DNA biology.This task demanding has not been fully exploited so far.Here we report a new method measuring using field-based light scattering spectroscopy, which applicable to any wavelength suitable both solutions homogenous objects with well-defined shape microspheres.The angular distribution single microspheres immersed homogeneous media...
Abstract Cell mass and its chemical composition are important aggregate cellular variables for physiological processes including growth control tissue homeostasis. Despite their central importance, it has been difficult to quantitatively measure these quantities from single cells in intact tissue. Here, we introduce Normalized Raman Imaging (NoRI), a Stimulated Scattering (SRS) microscopy method that provides the local concentrations of protein, lipid water live or fixed samples with high...
Nanogap electrodes-based dielectric spectroscopy is introduced to create ultrasensitive biomolecular sensors by minimizing the effects of electrode polarization. The polarization a major source error in determining impedance biological samples solution. unwanted double layer due impedance. caused accumulation ions on surface electrode. This effect becomes more dominant low frequency region (< 1 kHz). In this paper we describe nanogap measurements that can minimize since layers overlap and...
Abstract The majority of microbes on earth, whether they live in the ocean, soil or animals, are not growing, but instead struggling to survive starvation 1–6 . Some genes and environmental conditions affecting survival have been identified 7–13 , despite almost a century study 14–16 we do know which processes lead irreversible loss viability, maintenance counteract them how lifespan is determined from balance these opposing processes. Here, used time-lapse microscopy capture characterize...
Mechanical forces have been shown to influence cellular decisions grow, die, or differentiate, through largely mysterious mechanisms. Separately, changes in resting membrane potential observed development, differentiation, regeneration, and cancer. We now demonstrate that is the central mediator of response mechanical pressure. show acting on cell change biomass density, which turn alters potential. Membrane then regulates number density epithelia by controlling growth, proliferation,...
A versatile cryogenic (5 K) ultrahigh-vacuum (UHV) atomic force microscope (AFM) with tip-gated transport measurement capability has been developed. Using high-resolution (&lt;1.5μm) plan-view charge-coupled device (CCD) optics, and three planar piezomotors we achieved visually guided in situ alignments of a sample position respect to the AFM tip, laser beam cantilever quadrant photodiode. We made optical fiber feedthroughs lens assembly bring external light CCD illuminating onto sample....
In bacteria, algae, fungi, and plant cells, the wall must expand in concert with cytoplasmic biomass production, otherwise cells would experience toxic molecular crowding 1 , 2 or lyse. But how achieve expansion of this complex biomaterial coordination biosynthesis macromolecules cytoplasm remains unexplained 3 although recent works have revealed that these processes are indeed coupled 4,5 . Here, we report a striking increase turgor pressure growth rate E. coli suggesting speed cell is...