A5019707890- Digital Holography and Microscopy
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical measurement and interference techniques
- Optical Coherence Tomography Applications
- Erythrocyte Function and Pathophysiology
- Animal Disease Management and Epidemiology
- Photoacoustic and Ultrasonic Imaging
- Celiac Disease Research and Management
- SARS-CoV-2 and COVID-19 Research
- Blood properties and coagulation
- Optical Polarization and Ellipsometry
- Cellular Mechanics and Interactions
- Eosinophilic Esophagitis
- Spectroscopy and Chemometric Analyses
- Eosinophilic Disorders and Syndromes
- Esophageal Cancer Research and Treatment
- Gastroesophageal reflux and treatments
- Glycosylation and Glycoproteins Research
- Esophageal and GI Pathology
- Lipid Membrane Structure and Behavior
London School of Hygiene & Tropical Medicine
2024
World Health Organization
2024
Nationwide Children's Hospital
2018-2020
University of Maryland, Baltimore
2019
University of Maryland Medical Center
2019
The Ohio State University Wexner Medical Center
2018-2019
University of Illinois Hospital & Health Sciences System
2019
Nemours Children's Clinic
2014-2017
University of Central Florida
2014
Florida College
2014
We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping refractive index in live biological cells.Using a heterodyne Mach-Zehnder interferometer, we record complex field images light transmitted through sample with varying directions illumination.To quantitatively reconstruct map cells, apply based on Rytov approximation.In this way, effect is taken into account reconstruction process and diffraction-free high resolution are obtained throughout...
Ultrasensitive Raman measurements in single living cells are possible through exploiting the effect of surface-enhanced scattering (SERS). Colloidal gold particles (60 nm size) that deposited inside as “SERS-active nanostructures” result strongly enhanced signals native chemical constituents cells. Particularly strong field enhancement can be observed when colloidal form clusters. The allow a cell 400–1800 cm −1 range with 1-μm lateral resolution relatively short collection times (1 second...
Using novel interferometric quantitative phase microscopy methods, we demonstrate that the surface integral of optical associated with live cells is invariant to cell water content. Thus, provide an entirely noninvasive method measure nonaqueous content or “dry mass” living cells. Given extremely high stability microscope and femtogram sensitivity changes in cellular dry mass, this new technique not only ideal for quantifying growth but also reveals spatially resolved subcellular dynamics...
By use of the Fourier decomposition a low-coherence optical image field into two spatial components that can be controllably shifted in phase with respect to each other, new high-transverse-resolution quantitative-phase microscope has been developed. The technique transforms typical microscope, high accuracy and path-length sensitivity λ/5500, which is stable over several hours. results obtained on epithelial red blood cells demonstrate potential this instrument for quantitative...
The MHC class I–related Fc receptor, FcRn, mediates the intestinal absorption of maternal IgG in neonatal rodents and transplacental transport humans by receptor-mediated transcytosis. In mice rats, expression FcRn epithelial cells is limited to suckling period. We have recently observed, however, clear adult human intestine, suggesting a function for beyond life humans. tested this hypothesis using polarized T84 cell line as model epithelium. Immunocytochemical data show that present...
The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of cell’s morphology and mechanics. These properties, in turn, influence ability RBCs transport oxygen circulation. Current mechanical measurements rely on external loads. Here we apply noncontact optical interferometric technique quantify thermal fluctuations RBC membranes with 3 nm accuracy over broad range spatial temporal frequencies. Combining this...
We report an in situ method of probing the structure living epithelial cells, based on light scattering spectroscopy with polarized light. The makes it possible to distinguish between single backscattering from uppermost cells and multiply scattered spectrum component can be further analyzed provide histological information about such as size distribution cell nuclei their refractive index. These are valuable quantities' detect diagnose precancerous changes human tissues.
Background WHO, as requested by its member states, launched the Expanded Programme on Immunization (EPI) in 1974 to make life-saving vaccines available all globally.To mark 50-year anniversary of EPI, we sought quantify public health impact vaccination globally since programme's inception. MethodsIn this modelling study, used a suite mathematical and statistical models estimate global regional 50 years against 14 pathogens EPI.For modelled pathogens, considered coverage routine supplementary...
The clinicopathologic features of 24 tumors showing perivascular myoid differentiation are described. These included with histologic "infantile-type" myofibromatosis occurring in adult patients (8 cases), composite "hemangiopericytoma" and glomus tumor (9 a distinctive concentric proliferation spindle cells (7 cases). Evidence morphologic overlap among these groups suggests they closely related neoplasms that form single spectrum. Age lesions resembling infantile-type ranged from 23 to 67...
We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative imaging epi-fluorescence investigation of live cells. The DPF instrument consists an interference microscope, which is incorporated into conventional inverted microscope. images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to cell lifetime. potential the quantifying rapid nanoscale motions in cells demonstrated experiments...
To further define the indications for postoperative pelvic irradiation and chemotherapy, an analysis of influence extent tumor invasion into perirectal fat, lymphatic or venous vessel invasion, grade on clinical course patients with Stage T3N0 rectal cancer undergoing surgery was undertaken.From 1968 to 1985, 117 underwent resection curative intent. No patient received neoadjuvant adjuvant chemotherapy. Surgical specimens were assessed maximum depth involvement, grade. After these local...
Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal nonvanishing tension coefficient for RBCs, which increases as transition from discocytic shape to spherical shape. measured GUVs is, however, factor 4-24 smaller. By contrast, bending moduli have similar values. This is consistent with cytoskeleton confinement model, in inhibits membrane...