A5020711047- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Proteomics Techniques and Applications
- 3D Printing in Biomedical Research
- Autoimmune and Inflammatory Disorders
- Advanced Biosensing Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Soft tissue tumor case studies
- Protein Degradation and Inhibitors
- Eosinophilic Disorders and Syndromes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Gene Regulatory Network Analysis
- Mathematical Biology Tumor Growth
- Mass Spectrometry Techniques and Applications
- Zebrafish Biomedical Research Applications
- Digital Holography and Microscopy
- Autophagy in Disease and Therapy
- Axon Guidance and Neuronal Signaling
- ATP Synthase and ATPases Research
Bar-Ilan University
2012-2025
Institute of Nanotechnology
2014
Goodman (Japan)
2013
Harvard University
2009-2013
Center for Systems Biology
2009-2012
Mitochondrial membrane potential provides a valuable indicator of cells’ health and functional status. Cytometry- microscopy-based analyses, in combination with fluorescent probes, are widely used to study mitochondrial behavior related cellular pathways, most notably – apoptosis. The cyanine dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide) facilitates discrimination energized deenergized mitochondria because the normally green forms red aggregates...
A long-standing question in biology is whether there an intrinsic mechanism for coordinating growth and the cell cycle metazoan cells. We examined size distributions populations of lymphoblasts applied a mathematical analysis to calculate how rates vary with both cycle. Our results show that rate size-dependent throughout After initial suppression, rapid increase during G1 phase, followed by period constant exponential growth. The probability division varies independently age. conclude...
The Anaphase-Promoting Complex (APC) is an E3 ubiquitin ligase that regulates mitosis and G1 by sequentially targeting cell-cycle regulators for ubiquitination proteasomal degradation. mechanism of chain formation APC the resultant topology remains controversial. By using a single-lysine substrate to dissect ubiquitinated substrates, we find APC-catalyzed has intrinsic preference K11 linkage essential specificity determined E2 enzyme, UBE2S/E2-EPF, elongates chains after substrates are...
Cell size is a defining characteristic central to cell function and ultimately tissue architecture. The ability sort subpopulations of different sizes would facilitate investigation at genomic proteomic levels mechanisms by which cells attain maintain their size. Currently available sorters, however, cannot directly measure volume electronically, it therefore be desirable know the optical measurements that can made in such instruments provide best estimate volume. We investigated several...
Significance Accurate measurement of cell size is critical in studies growth. Optical methods based on interferometry are known to be suitable for attached cells, but the existing techniques were originally designed thin samples and not ideal thick ones, such as mitotic cells. Synthetic phase microscopy (SPM), a new tomographic interferometric method, offers an elegant solution this problem. This paper demonstrates ability SPM measure growth mammalian cells accurately, it clear requirement...
Article9 November 2017Open Access Transparent process Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell Eunyong Ahn Department of Computer Science, Technion, Haifa, Israel Search for more papers by this author Praveen Kumar Biology, Dzmitry Mukha Amit Tzur Faculty Life Sciences, Bar-Ilan University, Ramat Gan, The Institute Nanotechnology and Advanced Materials, Tomer Shlomi Corresponding Author [email protected] orcid.org/0000-0003-3491-5156 Lokey...
Animal cell division ends with the cutting of microtubule and membrane intercellular bridge connecting 2 daughter cells. This process, known as cytokinetic abscission (abscission), is widely regarded last step cytokinesis, i.e., cycle. Major breakthroughs have been recently achieved, illuminating mechanistic aspects abscission; however, timing respect to mammalian cycle remains unclear. In this study, we carefully measured onset progression in dividing cells expressing a G1 reporter. We...
Abstract Dynamic oscillations in the phosphorylation and ubiquitination of key proliferative regulators are defining features eukaryotic cell cycle. Resetting cycle at mitosis-to-G1 transition requires activation E3 ubiquitin ligase Anaphase-Promoting Complex/Cyclosome (APC/C), which ensures irreversibility by targeting dozens substrates for degradation, safeguarding genome integrity. However, overall coupling substrate with target recognition degradation APC/C remains relatively unexplored....
Currently, the reliable identification of peptides and proteins is only feasible when thoroughly annotated sequence databases are available. Although sequencing capacities continue to grow, many organisms remain without reliable, fully reference genomes required for proteomic analyses. Standard database search algorithms fail identify that not exactly contained in a protein database. De novo searches generally hindered by their restricted reliability, current error-tolerant strategies...
Different types of mature B-cell lymphocytes are overall highly similar. Nevertheless, some B cells proliferate intensively, while others rarely do. Here, we demonstrate that a simple binary classification gene expression in proliferating vs. resting can identify, with remarkable selectivity, global vivo regulators the mammalian cell cycle, many which also post-translationally regulated by APC/C E3 ligase. Consequently, discover novel regulatory network between and E2F transcription factors...
Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show the Gas2l3 is targeted for ubiquitin-mediated proteolysis by APC/C(Cdh1) complex, but not APC/C(Cdc20) phosphorylated Cdk1 in mitosis. Moreover, late cytokinesis, exclusively localized to constriction sites, which are narrowest parts of intercellular bridge connecting two daughter cells....
Cell cycle research greatly relies on synchronization of proliferating cells. However, effective mammalian cells is commonly achieved by long exposure to one or more cell blocking agents. These chemicals are, definition, hazardous (some than others), pose uneven arrest, thus introducing unwanted variables. The challenge synchronizing in G1 even greater; this process typically involves the release drug-arrested into that follows, a heterogeneous can truly limit synchronization. Moreover,...
We uncovered interlocking mechanisms regulating the temporal proteolysis of transcriptional repressor E2F8 in cycling cells including SCF Cyclin F G2, dephosphorylation Cdk1 sites, and activation APC/C Cdh1 , but not Cdc20 during mitotic exit G1. Differential stabilization under limited activity allows to reaccumulate late G1 coregulate S-phase entry.
Protein post-translational modifications mediate dynamic cellular processes with broad implications in human disease pathogenesis. There is a large demand for high-throughput technologies supporting research, and both mass spectrometry protein arrays have been successfully utilized this purpose. override the major limitation of target abundance inherently associated MS analysis. This technology, however, typically restricted to pre-purified proteins spotted fixed composition on chips limited...
Abstract Autophosphorylation of receptor and non-receptor tyrosine kinases is a common molecular switch with broad implications for pathogeneses therapy cancer other human diseases. Technologies large-scale discovery analysis autophosphorylation are limited by the inherent difficulty to distinguish between phosphorylation in vivo complexity associated functional assays receptors vitro. Here, we report method direct detection using integrated microfluidics freshly synthesized protein arrays....
Abstract Cytokinesis is an intensively studied process by which the cell cytoplasm divides to produce two daughter cells. Like any other aspect of cycle research, study cytokinesis relies heavily on synchronization. However, synchronization cells during challenging due rapid nature this and shortage blocking agents specifically targeting phase. Here, we demonstrate use standard flow cytometry for directly isolating cytokinetic from asynchronous population normally proliferating This approach...
Abstract Cancer is the second leading cause of death globally. Matching proper treatment and dosage crucial for a positive outcome. Any given drug may affect patients with similar tumors differently. Personalized medicine aims to address this issue. Unfortunately, most cancer samples cannot be expanded in culture, limiting conventional cell‐based testing. Herein, presented microfluidic device that combines microarray cell microscopy. The can perform 512 experiments test chemosensitivity...
Abstract E2F8 is a transcriptional repressor that antagonizes the canonical cell cycle transcription factor E2F1. Despite importance of this atypical E2F family member in cycle, apoptosis and cancer, we lack complete description mechanisms control its dynamics. To address question, developed complementary set static dynamic cell-free systems human origin, which recapitulate inter-mitotic G1 phases, full transition from pro-metaphase to G1. This revealed an interlocking molecular switch...