Significant advances in mass spectrometry imaging (MSI) have pushed the boundaries obtaining spatial information and quantification biological samples. Quantitative MSI (qMSI) has typically been challenging to achieve because of matrix tissue heterogeneity, inefficient analyte extraction, ion suppression effects, but recent studies demonstrated approaches obtain highly robust methods reproducible results. In this perspective, we share our insights into sample preparation, how choice...

Iron oxides, which are documented phosphorus (P) sinks as adsorbents, have been shown to catalyze organic P dephosphorylation, implicating these minerals catalytic traps in cycling. However, quantitative evaluation of this abiotic catalysis is lacking. Here, we investigated the dephosphorylation kinetics eight ribonucleotides, with different nucleobase structures and stoichiometry, reacting common iron oxides. X-ray absorption spectroscopy determined that 0–98% mineral-bound was recycled...

Abstract Altered lipid metabolism is one of the hallmarks cancer. Cellular proliferation and de novo synthesis lipids are related to cancer progression. In this study, we evaluated lipidomic profile two‐dimensional (2D) monolayer multicellular tumor spheroids from HCT 116 colon carcinoma cell line. We utilized serial trypsinization on spheroid samples generate three cellular populations representing proliferative, quiescent, necrotic regions spheroid. This analysis enabled a comprehensive...

Mass spectrometry imaging (MSI) is a tool to rapidly map the spatial location of analytes without need for tagging or reporter system. Niemann-Pick disease type C1 (NPC1) neurodegenerative, lysosomal storage disorder characterized by accumulation unesterified cholesterol and sphingolipids in endo-lysosomal Here, we use MSI visualize lipids including cerebellar brain tissue from NPC1 symptomatic mouse model unaffected controls. To complement studies, data-processing pipeline was developed...

Poly(glutamic acid) at low pH self-assembles after incubation higher temperature into fibrils composed of antiparallel sheets that are stacked in a β2-type structure whose amide carbonyls have bifurcated H-bonds involving the side chains from next sheet. Oligomers Glu can also form such structures, and isotope labeling has provided insight their out-of-register [ Biomacromolecules 2013 , 14 3880 - 3891 ]. In this paper we report IR VCD spectra transmission electron micrograph (TEM) images...

Many potential chemotherapeutics fail to reach patients. One of the key reasons is that compounds are tested during drug discovery stage in two-dimensional (2D) cell cultures, which often unable accurately model vivo outcomes. Three-dimensional (3D) vitro tumor models more predictive chemotherapeutic effectiveness than 2D and thus, their implementation screening has evaluate earlier, saving both time money. Paper-based cultures (PBCs) an emerging 3D culture platform cells suspended Matrigel...

Niemann-Pick disease type C1 (NPC1) is a lipid storage disorder in which cholesterol and glycosphingolipids accumulate late endosomal/lysosomal compartments because of mutations the NPC1 gene. A hallmark progressive neurodegeneration cerebellum as well visceral organ damage; however, mechanisms driving this pathology are not fully understood. Phosphoinositides phospholipids that play distinct roles signal transduction vesicle trafficking. Here, we utilized consensus spectra analysis MS...

A multi-glycomic method for characterizing the glycocalyx was employed to identify difference between 2-dimensional (2D) and 3-dimensional (3D) culture models with two human colorectal cancer cell lines, HCT116 HT29. 3D cultures are considered more representative of due their ability mimic microenvironment found in tumors. For this reason, they have become an important tool research. Cell-cell interactions increase compared 2D, indeed significant glycomic changes were observed each line....

Replacing water with dimethyl sulfoxide (DMSO) completely reshapes the free-energy landscapes of solvated proteins. In DMSO, a powerful hydrogen-bond (HB) acceptor, formation HBs between backbone NH groups and solvent is favored over involving protein's carbonyl groups. This entails profound structural disruption globular proteins proteinaceous aggregates (e.g., amyloid fibrils) upon transfer to DMSO. Here, we investigate an unusual DMSO-induced conformational transition β2-amyloid fibrils...

Lipids are essential macromolecules that play a crucial role in numerous biological events. structurally diverse which allows them to fulfill multiple functional roles. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is powerful tool understand the spatial localization of lipids within systems. Herein, we report use ammonium fluoride (NH4F) as comatrix additive enhance lipid detection samples, with signal increase up 200%. Emphasis was placed on anionic...

Cancerous cells synthesize most of their lipids de novo to keep up with rapid growth and proliferation. Fatty acid synthase (FAS) is a key enzyme in the lipogenesis pathway that upregulated many cancers has gained popularity as druggable target interest for cancer treatment. The first FAS inhibitor discovered, cerulenin, initially showed promise chemotherapeutic purposes until it was observed had adverse side effects mice. TVB-2640 (Denifanstat) part newer generation inhibitors. With...

MALDI-TOF mass spectrometry imaging (MSI) is a powerful tool for studying biomolecule localization in tissue. Protein distributions tissue provide important histological information; however, large proteins exhibit high limit of detection MALDI-MS when compared to their corresponding smaller proteolytic peptides. As result, several techniques have emerged digest into more detectable peptides imaging. Digestion typically accomplished through trypsin deposition on the tissue, but this...

Altered lipid metabolism is one of the hallmarks cancer. Cellular proliferation and de novo synthesis lipids are related to cancer progression. In this study, we evaluated lipidomic profile two-dimensional (2D) monolayer multicellular tumor spheroids from HCT 116 colon carcinoma cell line. We utilized serial trypsinization on spheroid samples generate three cellular populations representing proliferative, quiescent, necrotic regions spheroid. This analysis enabled a comprehensive...

Lipids are essential macromolecules that play a crucial role in numerous biological events. structurally diverse which allows them to fulfill multiple functional roles. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is powerful tool understand the spatial localization of lipids within systems. Herein, we report use ammonium fluoride (NH4F) as comatrix additive enhance lipid detection samples, with signal increase up 200%. Emphasis was placed on anionic...

Lipids are essential macromolecules that play a crucial role in numerous biological events. structurally diverse which allows them to fulfill multiple functional roles. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is powerful tool understand the spatial localization of lipids within systems. Herein, we report use ammonium fluoride (NH4F) as comatrix additive enhance lipid detection samples, with signal increase up 200%. Emphasis was placed on anionic...

Abstract Disclosure: N. Pascuali: None. F. Tobias: K. Valyi-Nagy: S. Salih: A. Veiga-Lopez: The incidence of infertility has been consistently increasing over the past few decades, with a high proportion these cases caused by ovarian-related dysfunction. Metabolic disorders such as hyper- or dyslipidemia have associated subfertility (e.g., follicular fluid is lower IVF success). Lipids play critical role in structure and function ovarian tissue. However, while several studies investigated...