A5000878853- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Marine Biology and Ecology Research
- Student Assessment and Feedback
- Methane Hydrates and Related Phenomena
- Marine Bivalve and Aquaculture Studies
- Evaluation and Performance Assessment
- Protist diversity and phylogeny
- Air Quality and Health Impacts
- Isotope Analysis in Ecology
- Ocean Acidification Effects and Responses
- Genomics and Phylogenetic Studies
- Educational Assessment and Improvement
- Enzyme Structure and Function
- Microbial Fuel Cells and Bioremediation
- Atmospheric and Environmental Gas Dynamics
- Metal Extraction and Bioleaching
- Odor and Emission Control Technologies
- Magnetic and Electromagnetic Effects
- Educational and Psychological Assessments
- Traffic and Road Safety
- Reflective Practices in Education
- Injury Epidemiology and Prevention
- Indoor Air Quality and Microbial Exposure
- Agriculture and Farm Safety
University of New Mexico
2023-2024
Scottish Universities Environmental Research Centre
2023-2024
Woods Hole Oceanographic Institution
2005-2020
North Middlesex Hospital
2016
Middlesex University
2016
University of Hawaii System
2015
University of Washington
1996-2014
University of Iowa
2001-2012
Appalachian State University
2012
Northeastern University
2011
ABSTRACT A coastal marine sulfide-oxidizing autotrophic bacterium produces hydrophilic filamentous sulfur as a novel metabolic end product. Phylogenetic analysis placed the organism in genus Arcobacter epsilon subdivision of Proteobacteria . This motile vibrioid can be considered difficult to grow, preferring grow under microaerophilic conditions flowing systems which sulfide-oxygen gradient has been established. Purified cell cultures were maintained by using this approach. Essentially all...
Coenzyme M is a recently discovered cofactor which involved in methyl transfer reactions Methanobacterium. Information derived from infrared, proton NMR, and ultraviolet spectroscopy as well chemical tests quantitative elemental analysis reveals that the coenzyme 2,2′-dithiodiethanesulfonic acid. Verification of this structure resides comparison authentic with chemically synthesized Evidence indicates an active form 2-mercaptoethanesulfonic acid methylated producing 2-(methylthio)...
Based on 16S rRNA gene surveys, bacteria of the epsilon subdivision proteobacteria have been identified to be important members microbial communities in a variety environments, and quite few demonstrated grow autotrophically. However, no information exists what pathway autotrophic carbon fixation these might use. In this study, Thiomicrospira denitrificans Candidatus Arcobacter sulfidicus, two chemolithoautotrophic sulfur oxidizers proteobacteria, were examined for activities key enzymes...
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Anthropogenically driven climate change will rapidly become Earth's dominant transformative influence in the coming decades. The oceanic biological pump—the complex suite of processes that results transfer particulate and dissolved organic carbon from surface to deep ocean—constitutes main mechanism for removing CO2 atmosphere sequestering at depth on submillennium time scales. Variations efficacy pump strength ocean sink, which is larger than all other bioactive reservoirs, regulate have...
Epidemiologic studies of farm children are international interest because less often atopic, have allergic disease, and asthma than do nonfarm children--findings consistent with the hygiene hypothesis. We studied a cohort rural Iowa to determine association between other environmental risk factors four outcomes: doctor-diagnosed asthma, asthma/medication for wheeze, current cough exercise. Doctor-diagnosed prevalence was 12%, but at least one these health outcomes found in more third cohort....
A growth factor present in rumen fluid and essential for of a strain Methanobacterium ruminantium was shown to be coenzyme M, 2-mercaptoethanesulfonic acid.
ABSTRACT During recent oceanographic cruises to Pacific hydrothermal vent sites (9°N and the Guaymas Basin), rapid microbial formation of filamentous sulfur mats by a new chemoautotrophic, hydrogen sulfide-oxidizing bacterium was documented in both situ shipboard experiments. Observations suggest that these may be factor initial colonization surfaces macrofaunal Alvinella worms. This novel metabolic capability, previously shown carried out coastal strain H 2 S continuous-flow reactors, an...
Using automated overwinter sampling devices, we collected preserved phytoplankton samples from multiple depths in Lake Fryxell, a permanently ice‐covered lake southern Victoria Land, Antarctica. Photosynthetic algae were maintained stable water column throughout winter darkness. The algal taxa overwintered different ways species‐specific manner. Typical vegetative cells the most abundant form for all species found column. Populations of one chlorophyte, Stichococcus sp., observed winter, but...
A highly motile chemoautotrophic strain of hydrogen sulfide – oxidizing bacteria from coastal seawater produces solid sulfur filaments dimensions 0.5 to 2.0 micrometers by 20 500 micrometers. Filamentous is rapidly produced direct excretion a vibrioid organism, and the newly are thickened deposition other members population. Microscopic observations flocculent discharge material collected diffuse-flow hydrothermal vents (9°N, East Pacific Rise) revealed that this source composed largely...
Summary Studies were conducted in opposing gradients of oxygen and sulfide microslide capillaries to (i) characterize the chemical microenvironment preferred by Candidatus Arcobacter sulfidicus, a highly motile, sulfur‐oxidizing bacterium that produces sulfur filamentous form, (ii) develop model describing mechanism filamentous‐sulfur formation. The motile microorganisms are microaerophilic, with swarms effectively aggregating within oxic‐anoxic interfaces exhibiting chemotactic response....
Metabolic transformations of glutamate and Casamino Acids by natural microbial populations collected from deep waters (1,600 to 3,100 m) were studied in decompressed undecompressed samples. Pressure-retaining sampling/incubation vessels appropriate subsampling/incubation subsampling techniques permitted time course experiments. In all cases the metabolic activity samples was lower than it when incubated at 1 atm. Surface water controls showed a reduced upon compression. The processes...
Abstract The methylation of 2,2'-dithiodiethanesulfonic acid ((S-CoM)2) by methylcobalamin-coenzyme M methyltransferase requires an additional acidic protein, when NADPH is used as the electron source. This protein component and may be replaced sodium borohydride which acts reducing (S-CoM)2 to 2-mercaptoethanesulfonic (HS-CoM) prior methylation. Evidence suggests that reduction chemical rather than enzyme directed. Catalysis 100-fold purified only methylcobalamin HS-CoM. assay accurate,...
The majority of environmental micro-organisms identified with the rRNA approach have never been visualized. Thus, their reliable classification and taxonomic assignment is often difficult or even impossible. In our preliminary 18S gene sequencing work from world's largest anoxic marine environment, Cariaco Basin (Caribbean Sea, Venezuela), we detected a ciliate clade, designated previously as CAR_H [Stoeck, S., Taylor, G. T. & Epstein, S. (2003). Appl Environ Microbiol 63, 5656-5663]. Here,...
The Dynamic Gait Index (DGI) measures the capacity to adapt gait complex tasks. current scoring system combining pattern (GP) and level of assistance (LOA) lacks clarity, test has a limited range measurement.This study developed new based on 3 facets performance (LOA, GP, time) examined psychometric properties modified DGI (mDGI).A cross-sectional, descriptive was conducted.Nine hundred ninety-five participants (855 patients with neurologic pathology mobility impairments [MI group] 140...