A5090192817- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- COVID-19 and healthcare impacts
- Topic Modeling
- Disaster Response and Management
- COVID-19 epidemiological studies
- COVID-19 and Mental Health
- Natural Language Processing Techniques
- Palliative Care and End-of-Life Issues
- Skin Protection and Aging
- Peptidase Inhibition and Analysis
- Plant-Microbe Interactions and Immunity
- Family and Patient Care in Intensive Care Units
- Travel-related health issues
- Infection Control and Ventilation
- COVID-19 Clinical Research Studies
- Multimodal Machine Learning Applications
- Dental Research and COVID-19
- COVID-19 Pandemic Impacts
- AI in Service Interactions
- Speech and dialogue systems
- Infection Control in Healthcare
- Nail Diseases and Treatments
- Scientific and Engineering Research Topics
- Childhood Cancer Survivors' Quality of Life
Ottawa Hospital
2024
University of Ottawa
2019-2024
Johnson Electric (China)
2021-2024
The University of Sydney
2013-2022
University of Maryland, Baltimore
2021-2022
Westmead Hospital
2013-2022
Taronga Conservation Society Australia
2017-2022
Sydney Local Health District
2019-2022
University of California, Santa Cruz
2021-2022
University of California, San Diego
2022
<h3>Objective:</h3> Our prespecified dose-response analyses of A Very Early Rehabilitation Trial (AVERT) aim to provide practical guidance for clinicians on the timing, frequency, and amount mobilization following acute stroke. <h3>Methods:</h3> Eligible patients were aged ≥18 years, had confirmed first (or recurrent) stroke, admitted a stroke unit within 24 hours onset. Patients randomized receive very early frequent mobilization, commencing hours, or usual care. We used regression...
Isolation and quarantine are key measures in outbreak management disease control. They are, however, associated with negative patient experiences outcomes, including an adverse impact on mental health lower quality of care due to limited interaction healthcare workers. In this study, we explore the lived experience perceptions patients isolation COVID-19 Australian setting.Using a phenomenological approach from Heideggerian hermeneutical perspective, conducted individual semistructured...
Inositol pyrophosphates (PP-IPs) comprising inositol, phosphate, and pyrophosphate (PP) are essential for multiple functions in eukaryotes. Their role fungal pathogens has never been addressed. Cryptococcus neoformans is a model pathogenic fungus causing life-threatening meningoencephalitis. We investigate the cryptococcal kinases responsible production of PP-IPs (IP7/IP8) hierarchy PP-IP importance pathogenicity. Using gene deletion inositol polyphosphate profiling, we identified Kcs1 as...
BackgroundCOVID-19 and its associated travel bans have reduced international passenger traffic by over 80% below 2019 levels. If airlines are to resume flying at commercially sustainable levels, they must work restore passengers confidence sense of security. This study examined commercial airline passengers' health concerns attitudes towards infection prevention control measures for safety in the current COVID-19 global pandemic.MethodsA cross-sectional was conducted inviting adult members...
Cryptococcal meningitis is fatal without treatment and responsible for more than 500,000 deaths annually. To be a successful pathogen, C. neoformans must obtain an adequate supply of essential nutrients, including phosphate, from various host niches. Phosphate acquisition in fungi regulated by the PHO signaling cascade, which activated when intracellular phosphate decreases below critical level. Induction genes leads to uptake free via transporters. By blocking pathway using Pho4...
Cryptococcus neoformans strains isolated from patients with AIDS secrete acid phosphatase, but the identity and role of enzyme(s) responsible have not been elucidated. By combining a one-dimensional electrophoresis step mass spectrometry, canonically secreted CNAG_02944 (Aph1), was identified in secretome highly virulent serotype A strain H99. We created an APH1 deletion mutant (Δaph1) showed that Δaph1-infected Galleria mellonella mice survived longer than those infected wild type (WT),...
Abstract Fungal inositol polyphosphate (IP) kinases catalyse phosphorylation of IP 3 to pyrophosphate, PP-IP 5 /IP 7 , which is essential for virulence Cryptococcus neoformans . Cryptococcal Kcs1 converts 6 but the kinase converting unknown. Deletion a putative kinase-encoding gene ( IPK1 ) alone ipk1 Δ) and in combination with KCS1 Δ kcs1 Δ), profoundly reduced mice. However, deletion had greater impact on attenuation than that alone. lung burdens were also lower those Δ. Unlike Δ, failed...
Abstract Background Returning home from the hospital for palliative‐focused care is a common transition, but process can be emotionally distressing and logistically challenging patients caregivers. While interventions exist to aid in none have been developed partnership with Objective To undergo initial stages of codesign create an intervention (Advancing Care Experience receiving Palliative as they Transition Home [ACEPATH]) improve experience hospital‐to‐home transitions adult palliative...
ABSTRACT Phospholipase C (PLC) of Cryptococcus neoformans (Cn Plc1 ) is crucial for virulence this fungal pathogen. To investigate the mechanism CnPlc1-mediated signaling, we established that phosphatidylinositol 4,5-bisphosphate (PIP 2 a major CnPlc1 substrate, which hydrolyzed to produce inositol trisphosphate (IP 3 ). In Saccharomyces cerevisiae , Plc1-derived IP substrate polyphosphate kinase Arg82, converts more complex polyphosphates. study, show in C. enzyme encoded by ARG1 kinase,...
Invasive fungal diseases pose a serious threat to human health globally with >1.5 million deaths occurring annually, 180,000 of which are attributable the AIDS-related pathogen, Cryptococcus neoformans . Here, we demonstrate that interaction inositol pyrophosphate, IP 7 , CDK inhibitor protein, Pho81, is instrumental in promoting virulence. -Pho81 stabilizes Pho81 association other complex components promote PHO pathway activation and phosphate acquisition. Our data demonstrating blocking...
Cryptococcus neoformans is a human fungal pathogen that adapts its metabolism to cope with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight into cryptococcal metabolism, we optimized protocol for the Seahorse Analyzer, which measures extracellular acidification rate (ECAR) consumption (OCR) as indications of glycolytic respiratory activities. In doing so achieved effective immobilization encapsulated cryptococci, established Rotenone/Antimycin A...
COVID-19 is a global health crisis. Close contact with the mucous membranes and respiratory secretions of patients aerosol-generating procedures renders dentists other oral professionals at high risk exposure to SARS-CoV-2. We examined dentists' knowledge, preparedness, experiences managing in Australia.A cross-sectional online survey current membership The Australian Dental Association (ADA) was conducted between March April 2021.Of 368 responses, most (72.3%) reported having good level...
Prompt-based or in-context learning has achieved high zero-shot performance on many natural language generation (NLG) tasks. Here we explore the of prompt-based for simultaneously controlling personality and semantic accuracy an NLG task-oriented dialogue. We experiment with PERSONAGE restaurant recommendation corpus to generate semantically stylistically-controlled text 5 different Big-5 types: agreeable, disagreeable, conscientious, unconscientious, extravert. test two classes discrete...
We previously identified a series of inositol polyphosphate kinases (IPKs), Arg1, Ipk1, Kcs1 and Asp1, in the opportunistic fungal pathogen Cryptococcus neoformans. Using gene deletion analysis, we characterized Ipk1 showed that they act sequentially to convert IP3 PP-IP5 (IP7), key metabolite promoting stress tolerance, metabolic adaptation dissemination brain. have now directly enzymatic activity demonstrating it is dual specificity (IP3/IP4) kinase producing IP5. IP5 further...
This tertiary analysis from A Very Early Rehabilitation Trial (AVERT) examined fatal and nonfatal serious adverse events (SAEs) at 14 days.AVERT was a prospective, parallel group, assessor blinded, randomized international clinical trial comparing mobility training commenced <24 hours poststroke, termed very early mobilization (VEM), to usual care (UC). Primary outcome assessed 3 months. Patients with ischemic or hemorrhagic stroke within 24 of onset were included. Treatment thrombolytics...
Australian dentists are among the frontline healthcare workers providing dental and oral health care during COVID-19 pandemic, therefore have been affected in multiple ways. In this study, we explore their experiences of practising living pandemic.A qualitative study analysed responses 333 dentists' who participated a survey with open-ended questions about challenges positive outcomes pandemic. The were embedded national online knowledge, preparedness conducted between March April 2021. Data...
COVID-19 has placed unprecedented demands on infection control professionals (ICPs) and infectious disease (ID) physicians. This study examined their knowledge, preparedness, experiences managing in the Australian healthcare settings.A cross-sectional of ICPs ID physician members Australasian College for Infection Prevention Control (ACIPC) Society Infectious Diseases (ASID) was conducted using an online survey. Descriptive statistics were used to summarise report data.A total 103 survey...