A5055190589- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- thermodynamics and calorimetric analyses
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- DNA and Nucleic Acid Chemistry
- Nanopore and Nanochannel Transport Studies
- Advanced Nanomaterials in Catalysis
- Hydrogels: synthesis, properties, applications
- Advanced Materials and Mechanics
- Advanced Thermodynamics and Statistical Mechanics
- Polyoxometalates: Synthesis and Applications
- Nanocluster Synthesis and Applications
- Spaceflight effects on biology
Fraunhofer Institute for Cell Therapy and Immunology
2020-2023
We report a chemically programmed design and the switching characteristics of functional metal–DNA-origami–polyoxometalate (POM) material obtained from solution-processed assembling biocompatible molecular precursors. The DNA origami is immobilized on gold surface via thiolate groups acts as carrier (ad-layer) structure, ensuring spatially controlled hybridization pre-defined six-helix bundle (6HB) positions with DNA-augmented, tris(alkoxo)-ligated Lindqvist-type polyoxovanadate (POV6)...
Abstract Solvent conditions are unexpectedly sufficient to drastically and reversibly slow down cells. In vitro on the molecular level, protein–solvent interactions change in presence of heavy water (D 2 O) its stronger hydrogen bonds. Adding D O cell medium living cells increases intracellular viscosity. While morphology phenotype remain unchanged, cellular dynamics transform into motion a changeable manner. This is exemplified slowdown proliferation migration, which caused by reversible...
Synthetic polycations are studied extensively as DNA delivery agents because of their ease production, good chemical stability, and low cost relative to viral vectors. This report describes the synthesis charge-shifting based on N,N-(dimethylamino)ethyl acrylate (DMAEA) 3-aminopropylmethacryamide (APM), called PAD copolymers, use for in vitro into HeLa cells. copolymers varying compositions were prepared by RAFT polymerization yield polymers controlled molecular weights with dispersities....
Single-filament tracing has been a valuable tool to directly determine geometrical and mechanical properties of entangled polymer networks. However, systematically verifying how the stiffness tracer filament or its molecular interactions with surrounding network impacts measurement these parameters not possible established experimental systems. Here, we use mechanically programmable DNA nanotubes embedded in crosslinked F-actin networks, as well synthetic order measure fundamental,...
Abstract We report the preparation and mechanical properties of highly swellable, spherical polymer microgels synthesized by precipitation copolymerization divinylbenzene‐55 (DVB), 4‐methylstyrene (4MS), maleic anhydride (MA) at different cross‐linker contents, in a range methylethylketone (MEK) heptane solvent mixtures. Microgels were characterized optical confocal microscopy, their tested using real‐time deformability cytometry (RT‐DC), technique developed to analyze cell measuring...
Entangled semiflexible polymer networks are usually described by the tube model, although this concept has not been able to explain all experimental observations. One of its major shortcomings is neglecting thermal fluctuations polymers surrounding examined test filament, such that disentanglement effects captured. In study, we present evidence correlated constraint release which predicted theoretically occurs in entangled, but crosslinked networks. By tracking single DNA nanotubes embedded...
In article number 2101840, Jörg Schnauß and co-workers show that heavy water (D2O) reversibly slows down cells, retards cell invasion, drastically impacts mechanics, possibly prolonging storage times for biological materials. The cellular dynamics, which transform into slow motion, can be captured via the time-temperature superposition principle, where temperature is replaced by D2O. Induced changes are fully reversible effects independent of signaling/expression.