A5076994778- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Wnt/β-catenin signaling in development and cancer
- Advanced Numerical Analysis Techniques
- Dynamics and Control of Mechanical Systems
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- RNA Research and Splicing
- Numerical methods for differential equations
- Kruppel-like factors research
Centre National de la Recherche Scientifique
2017-2020
Dynamique du noyau
2020
Institut Curie
2017-2020
Sorbonne Université
2017-2020
Université Paris Sciences et Lettres
2017-2020
Université Paris Cité
2017-2019
How the very first step in nucleosome assembly, deposition of histone H3-H4 as tetramers or dimers on DNA, is accomplished remains largely unclear. Here, we report that yeast chromatin assembly factor 1 (CAF1), a conserved chaperone complex deposits during DNA replication, binds single heterodimer solution. We identify new DNA-binding domain large Cac1 subunit CAF1, which required for high-affinity binding by CAF1 three-subunit complex, and distinct from previously described C-terminal...
Abstract Vertebrates exhibit specific requirements for replicative H3 and non-replicative H3.3 variants during development. To disentangle whether this involves distinct modes of deposition or unique functions once incorporated into chromatin, we combined studies in Xenopus early development with chromatin assays. Here investigate the extent to which mutated at residues that differ from H3.2 rescue developmental defects caused by depletion. Regardless pathway, only residue 31—a serine can...
Chromatin organization in the nucleus provides a vast repertoire of information addition to that encoded genetically. Understanding how this impacts genome stability and influences cell fate tumorigenesis is an area rapid progress. Considering nucleosome, fundamental unit chromatin structure, study histone variants (the bricks) their selective loading by chaperones architects) particularly informative. Here, we report recent advances understanding relationships between contribute using lines...
A hallmark of Wnt/β-Catenin signaling is the extreme diversity its transcriptional response, which varies depending on cell and developmental context. What controls this poorly understood. In all cases, switch from repression to activation depends a nuclear increase in β-Catenin, detaches transcription factor T 7 like 1 (Tcf7l1) bound Groucho (Gro) co-repressors DNA-binding sites transiently converts Tcf7/Lymphoid enhancer binding (Lef1) into activator. One earliest evolutionarily conserved...
Abstract The closely related replicative H3 and non-replicative H3.3 variants show specific requirement during development in vertebrates. Whether it involves distinct mode of deposition or unique roles once incorporated into chromatin remains unclear. To disentangle the two aspects, we took advantage Xenopus early combined with assays. Our previous work showed that Xenopus, depletion variant impairs at gastrulation, without compensation through provision H3.2. We systematically mutated each...