A5066862032- Retinal Development and Disorders
- Neuroscience of respiration and sleep
- Developmental Biology and Gene Regulation
- Neonatal Respiratory Health Research
- Wnt/β-catenin signaling in development and cancer
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Congenital heart defects research
- Hedgehog Signaling Pathway Studies
- Neuroscience and Neural Engineering
- Infant Health and Development
- RNA regulation and disease
- Hippo pathway signaling and YAP/TAZ
- Marine animal studies overview
- Cancer-related gene regulation
- Axon Guidance and Neuronal Signaling
- Kruppel-like factors research
- Cancer-related Molecular Pathways
- Genetics and Neurodevelopmental Disorders
- Renal and related cancers
- Ocular Disorders and Treatments
- Retinal and Macular Surgery
- Genetic and Kidney Cyst Diseases
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
Institut des Neurosciences Paris-Saclay
2015-2024
Université Paris-Saclay
2017-2024
Centre National de la Recherche Scientifique
2008-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2023
CEA Paris-Saclay
2023
Institut Curie
2017-2019
Université Paris Sciences et Lettres
2017-2019
Inserm
2013-2019
Université Paris-Sud
2008-2019
Délégation Paris 7
2013
Continuous neurogenesis in the adult nervous system requires a delicate balance between proliferation and differentiation. Although Wnt/β-catenin Hedgehog signalling pathways are thought to share mitogenic function neural stem/progenitor cells, it remains unclear how they interact this process. Adult amphibians produce retinal neurons from pool of stem cells localised ciliary marginal zone (CMZ). Surprisingly, we found that perturbations Wnt result opposite proliferative outcomes CMZ....
The retina of fish and amphibian contains genuine neural stem cells located at the most peripheral edge ciliary marginal zone (CMZ). However, their cell-of-origin as well mechanisms that sustain maintenance during development are presently unknown. We identified Hes4 (previously named XHairy2), a gene encoding bHLH-O transcriptional repressor, cell-specific marker Xenopus CMZ is positively regulated by canonical Wnt pathway negatively Hedgehog signaling. found retinogenesis, labels small...
During vertebrate neurulation, the embryonic ectoderm is patterned into lineage progenitors for neural plate, crest, placodes and epidermis. Here, we use Xenopus laevis embryos to analyze spatial temporal transcriptome of distinct ectodermal domains in course during establishment cell lineages. In order define small groups cells from a single germ layer retain information, dorsal ventral was subdivided along anterior-posterior medial-lateral axes by microdissections. Principal component...
Abstract Regenerative abilities are not evenly distributed across the animal kingdom. The underlying modalities also highly variable. Retinal repair can involve mobilization of different cellular sources, including ciliary marginal zone (CMZ) stem cells, retinal pigmented epithelium (RPE), or Müller glia. To investigate whether magnitude damage influences regeneration modality Xenopus retina, we developed a model based on cobalt chloride (CoCl 2 ) intraocular injection, allowing for...
The adult frog retina retains a reservoir of active neural stem cells that contribute to continuous eye growth throughout life. We found Yap, downstream effector the Hippo pathway, is specifically expressed in these cells. Yap knock-down leads an accelerated S-phase and abnormal progression DNA replication, phenotype likely mediated by upregulation c-Myc. This associated with increased occurrence damage eventually p53-p21 pathway-mediated cell death. Finally, we identified PKNOX1,...
Abstract Vertebrate retinal stem cells, which reside quiescently within the ciliary margin, may offer a possibility for treatment of degenerative retinopathies. The highly proliferative precursor cells in Xenopus eyes are confined to most peripheral region, called marginal zone (CMZ). Although canonical Wnt pathway has been implicated developing retina different species, little is known about its involvement postembryonic retinas. Using green fluorescent protein-based Wnt-responsive...
Retinitis pigmentosa (RP) is the most common inherited retinal disease (IRD) and characterized by photoreceptor degeneration progressive vision loss. We report 4 patients presenting with RP from 3 unrelated families variants in TBC1D32, which to date has never been associated an IRD. To validate TBC1D32 as a putative causative gene, we combined Xenopus vivo approaches human induced pluripotent stem cell-derived (iPSC-derived) models. Our data showed that was expressed during development it...
Neural crest (NC) specification comprises an early phase, initiating immature NC progenitors formation at neural plate stage, and a later phase fold resulting into functional premigratory NC, able to delaminate migrate. We found that the Gene Regulatory Network triggers up-regulation of pfkfb4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4) during this late phase. As shown in previous studies, PFKFB4 controls AKT signaling gastrulas glycolysis rate adult cells. Here, we focus on...
Wnt proteins form a family of highly conserved secreted molecules that are critical mediators cell-cell signaling during embryogenesis. Partial data on activity in different tissues and at stages have been reported frog embryos. Our objective here is to provide coherent detailed description throughout embryo development. Using transgenic Xenopus tropicalis line carrying Wnt-responsive reporter sequence, we depict the spatial temporal dynamics canonical We comprehensive series situ...
Neural stem cell research suffers from a lack of molecular markers to specifically assess or progenitor properties. The organization the Xenopus ciliary marginal zone (CMZ) in retina allows spatial distinction these two types: cells are confined most peripheral region, while progenitors more central. Despite this clear advantage, very few genes expressed retinal have been discovered so far model. To gain insight into signature cells, we performed large-scale expression screen CMZ,...
Retinitis pigmentosa is an inherited retinal dystrophy that ultimately leads to blindness due the progressive degeneration of rod photoreceptors and subsequent non-cell autonomous death cones. Rhodopsin most frequently mutated gene in this disease. We here developed rhodopsin editing-based models retinitis two Xenopus species, laevis tropicalis, by using CRISPR/Cas9 technology. In both them, loss function results massive cell characterized shortening outer segments occasional death. This...
Observations of knock-out mice suggest that breathing at birth requires correct development a specific hindbrain territory corresponding to rhombomeres (r) 3 and 4. Focusing on this territory, we examined the neuronal rhythm generator in chick embryo. We show rhythmic activity r4 is inducible after developmental stage 10 through interaction with r3. Although nature remains obscure, find expression Krox20 , segmentation gene responsible for specifying r3 r5, sufficient endow other capacity...
A hallmark of Wnt/β-Catenin signaling is the extreme diversity its transcriptional response, which varies depending on cell and developmental context. What controls this poorly understood. In all cases, switch from repression to activation depends a nuclear increase in β-Catenin, detaches transcription factor T 7 like 1 (Tcf7l1) bound Groucho (Gro) co-repressors DNA-binding sites transiently converts Tcf7/Lymphoid enhancer binding (Lef1) into activator. One earliest evolutionarily conserved...
Both androgens and estrogens are necessary to ensure proper testis development function. Studies on endocrine disruptors have highlighted the importance of maintaining balance between during fetal development, when is highly sensitive environmental disturbances. This regulated mainly through an enzymatic cascade that converts irreversibly into estrogens. The most important component this its terminal enzyme: cytochrome p450 19A1 (aromatase hereafter). study was conducted improve our...
Abstract How regional patterning of the neural tube in vertebrate embryos may influence emergence and function networks remains elusive. We have begun to address this issue embryonic mouse hindbrain by studying rhythmogenic properties different segments. isolated pre‐ post‐otic segments spinal tube, when they form at day (E) 9, grafted them into same positions stage‐matched chick hosts. Three days after grafting, vitro recordings activity cranial nerves exiting grafts indicate that a high...
Retinal neurodegenerative diseases are the leading causes of blindness. Among numerous therapeutic strategies being explored, stimulating self-repair recently emerged as particularly appealing. A cellular source interest for retinal repair is Müller glial cell, which harbors stem cell potential and an extraordinary regenerative capacity in anamniotes. This is, however, very limited mammals. Studying molecular mechanisms underlying regeneration animal models with capabilities should provide...
Summary statement PFKFB4 controls neural crest final specification and migration by regulation of AKT signaling or glycolysis. Abstract Neural (NC) comprises an early phase, initiating immature NC progenitors formation at plate stage, a later phase fold resulting into functional premigratory NC, able to delaminate migrate. We found that the NC-GRN triggers up-regulation pfkfb4 (6- p hosphofructo-2- k inase/ f ructose-2,6- b isphosphatase 4 ) during this late phase. As shown in previous...
Unlike mammals, some nonmammalian species recruit Müller glia for retinal regeneration after injury. Identifying the underlying mechanisms may help to foresee regenerative medicine strategies. Using a
Abstract Regenerative abilities are not evenly distributed across the animal kingdom. Interestingly, underlying modalities also highly variable, even among closely related species. In fish or amphibians, retinal repair can involve mobilization of different cellular sources, including stem cells ciliary marginal zone (CMZ), pigmented epithelial (RPE) cells, Müller glia. The mechanisms that trigger recruitment one cell type over another remain elusive. To investigate whether magnitude damage...