In adulthood, the action of androgens on seminiferous tubules is essential for full quantitatively normal spermatogenesis and fertility. contrast, their role in fetal testis, particularly germ cell development, remains largely unknown. Using testicular feminized (Tfm) mice, we investigated effects a lack functional androgen receptor (AR) cells, also named gonocytes. We demonstrated that endogenous androgens/AR physiologically control gonocyte proliferation. observed an increase number...

Histone H3 Lys 4 methylation (H3K4me) is deposited by the conserved SET1/MLL methyltransferases acting in multiprotein complexes, including Ash2 and Wdr5. Although individual subunits contribute to complex activity, how they influence gene expression specific tissues remains largely unknown. In Caenorhabditis elegans, SET-2/SET1, WDR-5.1, ASH-2 are differentially required for germline H3K4 methylation. Using profiling on germlines from animals lacking set-2, ash-2, or wdr-5.1, we show that...

The uniparental transmission of the maternal mitochondrial genome is achieved by disposal sperm mitochondria and their DNA (mtDNA) around fertilization. C. elegans embryo allowed identification several factors mechanisms involved after discovery that autophagy pathway its adaptor ALLO-1 are implicated represented a major progress. In addition, studies in fish fly have CPS-6, endonuclease, degradation sperm-derived mtDNA. mitochondria-associated proteins FNDC-1 PHB-2 were also efficiently...

The ubiquitin-proteolytic system controls the stability of proteins in space and time. In this study, using a temperature-sensitive mutant allele cul-2 gene, we show that CRL2LRR-1 (CUL-2 RING E3 ubiquitin-ligase Leucine Rich Repeat 1 substrate recognition subunit) acts at multiple levels to control germline development. promotes germ cell proliferation by counteracting DNA replication ATL-1 checkpoint pathway. also participates mitotic proliferation/meiotic entry decision, presumably...

It is currently admitted that Follicle-Stimulating Hormone (FSH) physiologically involved in the development and function of fetal/neonatal Sertoli cells rat but not mouse. However, FSH produced by both species from late fetal life onwards. We thus reinvestigated role mouse testis at day 0 (birth) 6, 8 10 post-partum (dpp) using mice lack functional receptors (FSH-R−/−). At birth, number proliferative index were significantly lower FSH-R−/− than wild type neonates. Claudin 11 mRNA expression...

The molecular mechanisms that regulate cell cycle progression in a developmental context are poorly understood. Here, we show the leucine-rich repeat protein LRR-1 promotes during C. elegans development, both germ line and early embryo. Our results indicate acts as nuclear substrate-recognition subunit of Cullin 2-RING E3 ligase complex (CRL2LRR-1), which ensures DNA replication integrity. contains typical BC/Cul-2 box binds CRL2 components vitro vivo box-dependent manner. Loss lrr-1...

During mammalian development, androgens produced by the fetal testis are most important hormones controlling masculinization of reproductive tract and genitalia. New findings show that male germ line is sensitive to anti-androgenic endocrine disruptors during embryonic period. In a recent study, we reported endogenous physiologically control cell growth in mouse fetus early life. present extended this result showing presence functional androgen receptor gonocytes latter part We also studied...

ABSTRACT The Notch signalling pathway is a conserved and widespread paradigm, its misregulation has been implicated in numerous disorders, including cancer. output of depends on the nuclear accumulation receptor intracellular domain (ICD). Using Caenorhabditis elegans germline, where GLP-1/Notch-mediated essential for maintaining stem cells, we monitored GLP-1 vivo. We found that enrichment ICD dynamic: while enriched germ cell nuclei during larval development, it depleted from adult...

Abstract Cullin-RING E3-Ligases (CRLs), the largest family of E3 ubiquitin-Ligases, regulate diverse cellular processes by promoting ubiquitination target proteins. The evolutionarily conserved Leucine Rich Repeat protein 1 (LRR-1) is a substrate-recognition subunit CRL2LRR-1 E3-ligase. Here we provide genetic evidence supporting role this E3-enzyme in maintenance DNA replication integrity Caenorhabditis elegans. Through RNAi-based suppressor screens lrr-1(0) and cul-2(or209ts) mutants,...

Loss of membrane potential sperm mitochondria has been regarded as the first step preceding mitophagy degradation after their entry into C. elegans oocyte at fertilization. This is in line with classical view defective or abnormal and could serve a recognition signal for specific quick autophagy degradation. Here, using TMRE (tetramethylrhodamine ethyl ester) live imaging we show that this not case. Instead, inherited stable labeling before time autophagosomes formation. Interestingly,...

Both androgens and estrogens are necessary to ensure proper testis development function. Studies on endocrine disruptors have highlighted the importance of maintaining balance between during fetal development, when is highly sensitive environmental disturbances. This regulated mainly through an enzymatic cascade that converts irreversibly into estrogens. The most important component this its terminal enzyme: cytochrome p450 19A1 (aromatase hereafter). study was conducted improve our...

The ubiquitin-proteolytic system (UPS) regulates a variety of cellular and biological processes by controlling the stability regulatory proteins, in space time. Not surprisingly, defects this have been associated with various syndromes pathologies, including cancer, illustrating importance understanding regulation multiple functions system. C. elegans is powerful model to identify components UPS study their function during development multicellular organisms. In elegans, evolutionarily...

Caenorhabditis elegans is a model system widely used in fundamental research. Even though, nematodes are easy to maintain the laboratory, obtaining large populations of worms require lot work and time consuming. Furthermore, because C. mainly hermaphrodite it even more complicated obtain amounts males which make high-throughput experiments using very challenging.In order overcome these limitations, we developed affordable rapid methods to:1) grow synchronous worm populations2) easily malesWe...

Caenorhabditis elegans is a model system widely used in fundamental research. Even though, nematodes are easy to maintain the laboratory, obtaining large populations of worms require lot work and time consuming. Furthermore, because C. mainly hermaphrodite it even more complicated obtain amounts males which make high-throughput experiments using very challenging.In order overcome these limitations, we developed affordable rapid methods to:(1) grow synchronous worm populations(2) easily...

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Escherichia coli est un hôte naturel de la flore intestinale des mammifères, très commun chez l’homme. Cependant, certaines souches sont pathogènes, comme les EPEC (E. entéropathogènes) qui provoquent infections intestinales à l’origine fortes diarrhées induisant une importante mortalité nouveau-nés et jeunes enfants dans pays en développement. Des travaux récents, tout fait fascinants, indiquent que ces bactéries pathogènes détournent cellule leur profit piratant acteur majeur du...

Escherichia coli est un hôte naturel de la flore intestinale des mammifères, très commun chez l'homme. Cependant, certaines souches sont pathogènes, comme les EPEC (E. entéropathogènes) qui provoquent infections intestinales à l'origine fortes diarrhées induisant une importante mortalité nouveau-nés et jeunes enfants dans pays en développement. Des travaux récents, tout fait fascinants, indiquent que ces bactéries pathogènes détournent cellule leur profit piratant acteur majeur du...

The molecular mechanisms that regulate cell cycle progression in a developmental context are poorly understood. Here, we show the leucine-rich repeat protein LRR-1 promotes during C. elegans development, both germ line and early embryo. Our results indicate acts as nuclear substrate-recognition subunit of Cullin 2-RING E3 ligase complex (CRL2LRR-1), which ensures DNA replication integrity. contains typical BC/Cul-2 box binds CRL2 components vitro vivo box-dependent manner. Loss lrr-1...